Characterization of Fusarium mangiferae isolates from mango malformation disease in Southern Spain
During the last years, Fusarium strains have been isolated from shoots and inflorescences of mango trees affected with floral and vegetative malformation in different orchards from the Axarquía region (south of Spain), highlighting the identification of Fusarium mangiferae. With the aim of elucidate epidemiological aspects and design more efficient control strategies, population diversity among the strains of F. mangiferae associated with MMD in Spain was determined by ap-PCR, RAPD-PCR, vegetative compatibility groups (VCGs) and mating type analyses. Three different VCGs were found among the Fusarium mangiferae Spanish isolates, two of them showing similar ap–PCR and RAPD profiles. PCR with primers specific for the mating type (MAT) alleles resulted in amplification of the MAT-2 allele fragment among the majority of the isolates, there being only two isolates MAT-1. This population diversity suggests at least three possible independent introductions of the pathogen into the Axarquía region.
KeywordsVCGs ap-PCR Fusarium mangiferae Population structure
This work was granted by CICE-Junta de Andalucía, Proyecto de Excelencia P07-AGR-02471, co-funded by FEDER funds (EU). This work was also financed by a grant from SAT-2803 TROPS, Reyes Gutiérrez S. L. and Viveros Brokaw S. L. The authors particularly acknowledge for their contribution in the sampling works to J. M. Hermoso, E. Guirado, J. González, (IHSM La Mayora-UMA-CSIC) and D. Sarmiento, (SAT-2803 TROPS). Also we wish to thanks Dr. Ploetz (University of Florida, USA) for gifted the VCGs reference F. mangiferae isolates. M. Crespo also was supported by a grant from University of Málaga for a short stay in the Volcani Center, Bet Dagan, Israel.
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