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European Journal of Plant Pathology

, Volume 139, Issue 2, pp 253–259 | Cite as

Characterization of Fusarium mangiferae isolates from mango malformation disease in Southern Spain

  • M. Crespo
  • E. Arrebola
  • F. M. Cazorla
  • M. Maymon
  • S. Freeman
  • J. A. Torés
  • A. de Vicente
Article

Abstract

During the last years, Fusarium strains have been isolated from shoots and inflorescences of mango trees affected with floral and vegetative malformation in different orchards from the Axarquía region (south of Spain), highlighting the identification of Fusarium mangiferae. With the aim of elucidate epidemiological aspects and design more efficient control strategies, population diversity among the strains of F. mangiferae associated with MMD in Spain was determined by ap-PCR, RAPD-PCR, vegetative compatibility groups (VCGs) and mating type analyses. Three different VCGs were found among the Fusarium mangiferae Spanish isolates, two of them showing similar ap–PCR and RAPD profiles. PCR with primers specific for the mating type (MAT) alleles resulted in amplification of the MAT-2 allele fragment among the majority of the isolates, there being only two isolates MAT-1. This population diversity suggests at least three possible independent introductions of the pathogen into the Axarquía region.

Keywords

VCGs ap-PCR Fusarium mangiferae Population structure 

Notes

Acknowledgments

This work was granted by CICE-Junta de Andalucía, Proyecto de Excelencia P07-AGR-02471, co-funded by FEDER funds (EU). This work was also financed by a grant from SAT-2803 TROPS, Reyes Gutiérrez S. L. and Viveros Brokaw S. L. The authors particularly acknowledge for their contribution in the sampling works to J. M. Hermoso, E. Guirado, J. González, (IHSM La Mayora-UMA-CSIC) and D. Sarmiento, (SAT-2803 TROPS). Also we wish to thanks Dr. Ploetz (University of Florida, USA) for gifted the VCGs reference F. mangiferae isolates. M. Crespo also was supported by a grant from University of Málaga for a short stay in the Volcani Center, Bet Dagan, Israel.

Supplementary material

10658_2014_398_Fig4_ESM.jpg (17 kb)
Supplementary Fig. 1

Band profiles generated from ap-PCR analysis with (CAG)5 repeat motif primer. Lanes 1 and 2, Fusarium spp. Spanish isolates (F0927 and F1194); lane 3, Fusarium tupiense CML262 from Brazil; lane 4, Fusarium mangiferae Spanish isolate (F0910, all the rest of the F. mangiferae Spanish isolate showed identical profile, Table 1); lane 5, F. mangiferae MRC7560 from Israel; lane 6, Fusarium sterilihyphosum MRC2802 from South Africa; line 7, Fusarium mexicanum GOC521 from Mexico. Outer marker lanes are 1Kb. (JPEG 16 kb)

10658_2014_398_MOESM1_ESM.tif (2.1 mb)
High ressolution image (TIFF 2158 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2014

Authors and Affiliations

  • M. Crespo
    • 1
  • E. Arrebola
    • 2
  • F. M. Cazorla
    • 1
  • M. Maymon
    • 3
  • S. Freeman
    • 3
  • J. A. Torés
    • 2
  • A. de Vicente
    • 1
  1. 1.Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga, Consejo Superior de Investigaciones Científicas, Departamento de Microbiología, Facultad de CienciasUniversidad de MálagaMálagaSpain
  2. 2.Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga, Consejo Superior de Investigaciones Científicas, Estación Experimental “La Mayora”MálagaSpain
  3. 3.Department of Plant Pathology and Weed Research, AROThe Volcani CenterBet DaganIsrael

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