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High level resistance to Pseudocercosporella capsellae offers new opportunities to deploy host resistance to effectively manage white leaf spot disease across major cruciferous crops

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Abstract

Field and controlled environment studies were undertaken to define the range and extent of available host resistances to Pseudocercosporella capsellae (white leaf spot) across diverse oilseed, forage and vegetable crucifers, including some wild and/or weedy species, and also within and/or derived from Brassica carinata. In each experiment, there was a wide range in host response from high resistance to high susceptibility as assessed by four disease parameters, viz. in the field for: (i) Area Under Disease Progress Curve (AUDPC) for percent leaves diseased with values ranging from 0 to 375.5; (ii) Percent Leaf Collapse Index (%LCI) for leaf collapse due to disease with values ranging from 0 to 23.0; and (iii), Percent Pod Area Disease Index (%PADI) for pod area affected with values ranging from 0 to 52.1; and (iv) under controlled environmental conditions for Percent Cotyledon Disease Index (%CDI) for cotyledon lesion size with values ranging from 0 to 27.5. At the Crawley field site, B. carinata ATC 94129 was the most resistant genotype with AUDPC = 1.2, followed by Crambe abyssinica (AUDPC 8.7), Eruca sativa Eruc-01 (AUDPC 19.3) and E. vesicaria Yellow rocket (AUDPC 19.4). B. carinata ATC 94129 and B. oleracea var. capitata had the least leaf collapse, with %LCI = 0.2. At the Shenton Park field site, 21 genotypes of B. carinata and B. oleracea var. acephala Tuscan kale showed total resistance, all with AUDPC values of 0. Of the B. napus genotypes carrying one or more B. carinata B genome introgressions, genotypes NC8 (AUDPC 23.0) and NC9-1 (AUDPC 26.2) were the most resistant. Genotypes as assessed on these disease criteria as having high level resistance generally showed no pod infection; in contrast to %PADI values up to 52 on the most susceptible genotypes. Under controlled environmental conditions, the most resistant genotype was B. carinata ATC 94129 with %CDI values of 0 and 0.2, respectively, across two experiments, along with B. napus genotypes Zhongyou 821 and Hyola 42, with a %CDI value of 0 in one of the two experiments. There was a high degree of correlation both within individual experiments across the different disease parameters and also between field and controlled environment experiments. Within both B. napus and B. juncea genotypes tested, the most resistant genotypes were from China, the most susceptible from India, with those from Australia intermediate.

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Acknowledgments

The first author gratefully acknowledges the financial assistance of an International SIRF Scholarship funded jointly by the Australian Government and The University of Western Australia. We appreciate the operational funding support for this research provided by the School of Plant Biology at The University of Western Australia. We thank Dr Huang Yi, Oil Crops Institute, China Academy of Agricultural Science, Wuhan, China for provision of seed lines with prefix YM from China; Dr Bob Redden, Curator, Australian Temperate Field Crops Collection, Horsham, Victoria for supplying seed lines with the ATC prefix; and Drs Phil Salisbury and Allison Gurung for also supplying some of the seed lines from the previous ACIAR program. We gratefully acknowledge the provision of half the salary of Martin Barbetti by the Department of Agriculture and Food Western Australia while these studies were undertaken.

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Authors and Affiliations

  1. School of Plant Biology, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Niroshini Gunasinghe, Ming Pei You & Martin J. Barbetti

  2. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004, Punjab, India

    Surinder S. Banga

  3. The UWA Institute of Agriculture, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Martin J. Barbetti

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  1. Niroshini Gunasinghe
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  2. Ming Pei You
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  3. Surinder S. Banga
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  4. Martin J. Barbetti
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Correspondence to Martin J. Barbetti.

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Gunasinghe, N., You, M.P., Banga, S.S. et al. High level resistance to Pseudocercosporella capsellae offers new opportunities to deploy host resistance to effectively manage white leaf spot disease across major cruciferous crops. Eur J Plant Pathol 138, 873–890 (2014). https://doi.org/10.1007/s10658-013-0360-y

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