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Anti-Phytophthora cinnamomi activity of Phlomis purpurea plant and root extracts

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Abstract

Phlomis purpurea (Lamiaceae), found in Quercus suber and Quercus ilex ssp. rotundifolia forest habitats in southern Portugal, is a non-host for the oomycete Phytophthora cinnamomi, the main biotic factor involved in cork oak and holm oak decline in the Iberian Peninsula. The effect of P. purpurea crude ethanol root extract was evaluated in vitro on P. cinnamomi mycelial growth, sporangial production, zoospore release and germination as well as on chlamydospore production and viability. The protection of cork oak against infection by the pathogen was also evaluated in planta. At 10 mg ml−1, in vitro inhibition of the pathogen structures was 85–100 %. In addition, P. purpurea plants were shown to protect Q. suber and Q. ilex from P. cinnamomi infection and to reduce the inoculum potential in glasshouse trials, indicating the ability to reduce root infection by the pathogen. The results suggest that P. purpurea has the potential to reduce disease spread and that their root extracts could provide candidate substances for control of the important pathogen, P. cinnamomi.

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Acknowledgments

The present work was financed by the Portuguese Ministério da Ciência e do Ensino Superior (MCES) (PTDC/AGR- CFL/100217/2008). D. Neves thanks Fundação para a Ciência e a Tecnologia (FCT) for her grant (SFRH/BD/66016/2009). The authors thank COST action FP 0801—Established and Emerging Phytophthora: Increasing Threats to Woodland and Forest Ecosystems in Europe, for supporting the participation in COST meetings to present and discuss part of the results disclosed in this paper. The authors are also grateful to Susana Durães for technical support in the greenhouse assays, to Pablo Hardoim for his support on statistics and to the nursery ‘Viveros de la Junta de Andalucía – Sevilla’ for gifting many of the plants used in this work.

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Authors and Affiliations

  1. Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    Dina Neves, Cristiana Maia, Nelson Sousa & Lídia Dionísio

  2. Institute for Biotechnology and Bioengineering - Centre of Genomics and Biotechnology, Plant and Animal Genomic Group, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    Marília Horta & Alfredo Cravador

  3. Department of Ecology, Environment and Plant Sciences, Stockholm University, Lilla Frescati, SE-106 91, Stockholm, Sweden

    Marco Salgado

  4. Applied Mycology Group, Cranfield Health, Cranfield University, Bedford, MK43 0AL, UK

    Naresh Magan

  5. Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Pólo da Mitra, Apartado 94, 7002-554, Évora, Portugal

    Lídia Dionísio

  6. ILRI (International Livestock Research Institute), Forage Genebank, CMC road, Gurdoshola, P.O Box 5689, Addis Ababa, Ethiopia

    Paula Caetano

  7. University College Cork, Microbiology Office, Room FSB452, 4th Floor, Food Science & Technology Building, Cork, Ireland

    Joana Oliveira

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  1. Dina Neves
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  2. Paula Caetano
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  3. Joana Oliveira
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  4. Cristiana Maia
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  5. Marília Horta
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  6. Nelson Sousa
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  7. Marco Salgado
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  10. Alfredo Cravador
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Correspondence to Dina Neves.

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Neves, D., Caetano, P., Oliveira, J. et al. Anti-Phytophthora cinnamomi activity of Phlomis purpurea plant and root extracts. Eur J Plant Pathol 138, 835–846 (2014). https://doi.org/10.1007/s10658-013-0357-6

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  • DOI: https://doi.org/10.1007/s10658-013-0357-6

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