Abstract
In Argentina, Thecaphora frezii is the causal agent of peanut smut, causing severe yield losses in peanut growing areas. In this work sequence data of the large ribosomal subunit (28S rDNA) of four T. frezii specimens from different production regions are provided. A fragment of about 650 bp was amplified by PCR from each of the isolates analyzed. The analysis of the sequences demonstrated 100 % identity among the four specimens and 92–96.3 % identity with sequences of other species of Thecaphora. This is the first study on molecular sequences of this pathogen.
References
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410.
Begerow, D., & Bauer, R. (2000). Phylogenetic placements of ustilaginomycetous anamorphs as deduced from nuclear LSU rDNA sequences. Mycological Research, 104, 53–60.
Begerow, D., Bauer, R., & Oberwinkler, F. (1997). Phylogenetic studies on nuclear LSU rDNA sequences of smut fungi and related taxa. Canadian Journal of Botany, 75, 2045–2056.
Carranza, J. M., & Lindquist, J. C. (1962). Thecaphora frezii sp., parásita de Arachis sp. Boletín de la Sociedad Argentina de Botánica, X(1), 11–17.
Doyle, J. J., & Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13–15.
Horton, T. R., & Bruns, T. D. (2001). The molecular revolution in ectomycorrhizal ecology: peeking into the black-box. Molecular Ecology, 10(8), 1855–1871.
Kendall, J. M., & Rygiewicz, P. T. (2005). Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts. BMC Microbiology, 5(28), 1–11.
Marinelli, A., & March, G. (2005). El carbón del maní. In G. J. March & A. D. Marinelli (Eds.), Enfermedades del maní en la Argentina (pp. 88–89). Córdoba: Biglia Impresiones.
Marinelli, A., March, G., & Rago, A. (1995). El carbón del maní Thecaphora frezii, sobre Arachis hypogaea L., Resúmenes VII Congreso de Micología y XVII Jornadas Argentinas de Micología. Rosario Santa Fe p. 134.
Marinelli, A., March, G., & Oddino, C. (2008). Aspectos biológicos y epidemiológicos del carbón del maní (Arachis hypogaea L.) causado por Thecaphora frezii Carranza & Lindquist. Agriscientia, 25, 1–5.
Matheny, P. B., Gossmann, J. A., Zalar, P., Arun Kumar, T. K., & Hibbett, D. S. (2006). Resolving the phylogenetic position of the Wallemiomycetes: an enigmatic major lineage of Basidiomycota. Canadian Journal of Botany, 84, 1794–1805.
O’Donnell, K. L. (1992). Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum (Gibberella pulicaris). Current Genetics, 22, 213–220.
O’Donnell, K. L. (1993). Fusarium and its near relatives. In D. R. Reynolds & J. W. Taylor (Eds.), The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics (pp. 225–233). Wallingford: CAB International.
Oddino, C., Marinelli, A., March, G., García, J., Tarditi, L., D’Eramo, L., & Ferrari, S. (2010). Relación entre el potencial inóculo de Thecaphora frezii. La intensidad de carbón del maní y el rendimiento del cultivo. 25° Jornada de Maní. General Cabrera, Córdoba. CIA-INTA. pp. 24–26
Park, H. S., Kim, G. Y., Nam, B. H., Lee, S. J., & Lee, J. D. (2002). The determination of the partial 28S ribosomal DNA sequences and rapid detection of Phellinus linteus and related species. Mycobiology, 30(2), 82–87.
Piepenbring, M. (2003). Smut fungi (Ustilaginomycetes p.p. and Microbotryales, Basidiomycota). Flora Neotropica Monograph 86. New York: New York Botanical Garden Press. 291 pp.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.
Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position -specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.
Vánky, K., & Lutz, M. (2007). Revision of some Thecaphora species (Ustilaginomycotina) on Caryophyllaceae. Mycological Research, 111, 1207–1219.
Vánky, K., Lutz, M., & Bauer, R. (2008). About the genus Thecaphora (Glomosporiaceae) and its new synonyms. Mycological Progress, 7, 31–39.
Acknowledgments
This work is dedicated to memory of Dr. Adriana Marinelli, for his teachings and knowledge in peanuts crop diseases. Also thank the collaboration of Ing. GuillermoMarch Ing. Mónica Astiz Gazzó, Fundación ArgenINTA and Fundación Maní Argentino. This research was supported by INTA (Instituto Nacional de Tecnología Agropecuaria) Programa Nacional de Cultivos Industriales, Proyecto Específico: Herramientas para el desarrollo de tácticas y estrategias de manejo de plagas y enfermedades limitantes en Cultivos Industriales.
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Conforto, C., Cazón, I., Fernández, F.D. et al. Molecular sequence data of Thecaphora frezii affecting peanut crops in Argentina. Eur J Plant Pathol 137, 663–666 (2013). https://doi.org/10.1007/s10658-013-0296-2
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DOI: https://doi.org/10.1007/s10658-013-0296-2