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Screening of cotton germplasm for resistance to Verticillium dahlia Kleb. under greenhouse and field conditions

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Abstract

Currently, the most effective and economically feasible means of controlling Verticillium wilt is to grow wilt-resistant cultivars. Nevertheless, resistant germplasm lines that are able to overcome this detrimental disease remain rare. Hence, we aimed to identify high-quality germplasm lines that exhibit high resistance, to address the dearth in optimum production. Our field and greenhouse studies showed significant variation among all tested germplasm lines with respect to wilt-assessment parameters. Highly resistant or resistant germplasm included 1421Bt-4133, O Siv2, Arcot-1, and GP93; Zhong 21371 and Yumian 2067 were moderately responsive; and Arcot402bne, Arcot438, Xinluzao-3, and Jimian11 were highly susceptible to wilt. Overall, germplasm line 1421Bt-4133 appeared to be an ideal material with strong resistance potential against V. dahliae. The germplasm lines O Siv2, Arcot-1, GP93, Zhong 21371, and Yumian 2067 showed variability in resistance potential in artificially or naturally-infested soil. This variability may be due to the genetic potential of the plants and to the adaptability of V. dahliae. We determined that the 1421Bt-4133 germplasm line was ideal, as it demonstrated resistance diversity against V. dahliae in all observed wilt-assessment parameters. The results of this study contribute to the understanding of viable germplasm lines for improving cotton production. The resistant germplasm lines uncovered in this study may provide new disease resistance sources in the breeding program.

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Acknowledgments

We thank Ms. Zhao Pan, Dr. He Qin Zhu and Lin Zhou for their invaluable technical help and support regarding this research. The authors acknowledge the support of the National High-Tech R&D Program of China (863 Program) (Grant number 2011AA10A102) and Crop Germplasm Conservation Program of the Ministry of Agriculture (NB2012-2130135-30). The authors also thank the Chinese Scholarship Council (CSC) of the China Government for supporting the entire study.

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  1. Crop Germplasm, State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences (ICR.CAAS), Anyang, Henan, 455000, China

    M. Ibrahim Khaskheli, J. Ling Sun, S. Pu He & X. Ming Du

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  1. M. Ibrahim Khaskheli
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Correspondence to X. Ming Du.

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Khaskheli, M.I., Sun, J.L., He, S.P. et al. Screening of cotton germplasm for resistance to Verticillium dahlia Kleb. under greenhouse and field conditions. Eur J Plant Pathol 137, 259–272 (2013). https://doi.org/10.1007/s10658-013-0235-2

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