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The role of seed infection level and fungicide seed treatments in control of net blotch in barley

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Abstract

The effect of seed-borne net blotch infection in barley on the agronomic characters yield, thousand kernel weight, grain volume weight, protein, starch and plant density was investigated in field trials using six net blotch infection levels (100/0, 80/20, 60/40, 40/60, 20/80 and 0/100 % high/low infected seed) and four fungicide seed treatments. The untreated trials showed significant differences between infection levels only for some agronomic characters, years and levels. Seed treatment with Anchor (carboxin + thiram) reduced net blotch by nearly 100 %, compared to 81 % by Fungazil A 25 (imazalil), 60 % by Rancona i-MIX (ipconazole + imazalil) and 54 % by Rubin TT (triticonazole + prochloraz + pyrimethanil). Anchor significantly increased yield 3–7 %, thousand kernel weight 3–13 %, grain volume weight 3–9 % and protein content 1–7 % in the first year, but not in the second. Fungazil A 25 had a low positive effect on agronomic characters, while Rancona i-MIX and Rubin TT had a positive, negative or no effect. The correlation between percent infected kernels in the conventional osmotic method and real-time PCR results was high, showing a potential for complementing traditional plate test methods with molecular methods that needs further optimization. The development of net blotch and its effect on agronomic characters thus depended on many other factors than the seed infection level. The current recommendations for seed treatment could be considered a preventative measure.

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References

  • Bates, J. A., Taylor, E. J. A., Kenyon, D. M., & Thomas, J. E. (2001). The application of a real-time PCR to the identification, detection and quantification of Pyrenophora species in barley seed. Molecular Plant Pathology, 2, 49–57.

    Article  PubMed  CAS  Google Scholar 

  • Berg, G., Holmblad, J., & Wikström, M. (2010). Växtskyddsåret 2010. Halland, Skåne och Blekinge län. Jönköping: Jordbruksverket.

    Google Scholar 

  • Carmona, M., Barreto, D., Moschini, R., & Reis, E. (2008). Epidemiology and control of seed-borne Drechslera teres on barley. Cereal Research Communications, 36(4), 637–645.

    Article  CAS  Google Scholar 

  • Carmona, M. A., Barreto, D. E., & Reis, E. M. (1999). Detection, transmission and control of Drechslera teres in barley seed. Seed Science & Technology, 27, 761–769.

    Google Scholar 

  • Clear, R. M., Patrick, S. K., & Gaba, D. (2000). Prevalence of fungi and fusariotoxins on barley seed from western Canada, 1995 to 1997. Canadian Journal of Plant Pathology, 22, 44–50.

    Article  Google Scholar 

  • EPPO. (2004). Efficacy evaluation of fungicides. Seed-borne cereal fungi. EPPO Standards. Efficacy of evaluation of plant protection products. Vol. 2. Fungicides & Bactericides. European and Mediterranean Plant Protection Organization PP 1/19, 4, 28–31.

    Google Scholar 

  • Ewaldz, T., Berg, G., & Baumgardt, M. (2008). Växtskyddsåret 2008. Halland, Skåne och Blekinge län. Jönköping: Jordbruksverket.

    Google Scholar 

  • Forsberg, G., Johnsson, L., & Lagerholm, J. (2005). Effects of aerated steam seed treatment on cereal seed-borne diseases and crop yield. Journal of Plant Diseases and Protection, 112(3), 247–256.

    Google Scholar 

  • Gerhardson, B. (2002). Biological substitutes for pesticides. Trends in Biotechnology, 20, 338–343.

    Article  PubMed  CAS  Google Scholar 

  • Gustafsson, G., & Djurberg, A. (2008). Växtskyddsåret 2008. Södermanland Östergötland Örebro län. Jönköping: Jordbruksverket.

    Google Scholar 

  • Gustafsson, G., & Djurberg, A. (2009). Växtskyddsåret 2009. Södermanland Östergötland Örebro län. Jönköping: Jordbruksverket.

    Google Scholar 

  • Gustafsson, G., & Djurberg, A. (2010). Växtskyddsåret 2010. Södermanland Östergötland Örebro län. Jönköping: Jordbruksverket.

    Google Scholar 

  • Hampton, J. C. (1980). The role of seed-borne inoculum in the epidemiology of net blotch of barley in New Zealand. New Zealand Journal of Experimental Agriculture, 8, 297–299.

    Article  Google Scholar 

  • Hökeberg, M. (1998). Seed bacterization for control of fungal seed-borne diseases in cereals. Doctoral thesis, Swedish University of Agricultural Sciences, Agraria 15

  • Jalli, M., & Robinson, J. (2000). Stable resistance in barley to Pyrenophora teres f. teres isolates from the Nordic-Baltic region after increase on standard host genotypes. Euphytica, 113, 71–77.

    Article  Google Scholar 

  • Jayasena, K. W., Loughman, R., & Majewski, J. (2002). Evaluation of fungicides in control of spot-type net blotch on barley. Crop Protection, 21, 63–69.

    Article  CAS  Google Scholar 

  • Joelson, G. (1983). The osmotic method—a method for rapid determination of seedborne fungi. Preprint No. 104, 20th ISTA Congress, Ottawa. June 17–25, 1983. (9 pp.) International Seed Testing Association, Zürich, Switzerland.

  • Jonsson, R., Bryngelsson, T., & Gustafsson, M. (1997). Virulence studies of Swedish net blotch isolates (Drechslera teres) and identification of resistant barley lines. Euphytica, 94, 209–218.

    Article  Google Scholar 

  • Jordbruksverket. (2012). Vid certifiering av stråsäd tillämpar Utsädesenheten följande gränser för betning. http://www2.sjv.se/download/18.50fac94e137b68090848000431/Betningskrav+SJV+2012+%C3%A4ndrad+juni.pdf. Accessed 19 May 2013.

  • Lantmännen. (2012). Cedomon. http://www.bioagri.se/cedomon.html. Accessed 19 September 2012.

  • Leisova, L., Minarikova, V., Kucera, L., & Ovesna, J. (2006). Quantification of Pyrenophora teres in infected barley leaves using real-time PCR. Journal of Microbiological Methods, 67, 446–455.

    Article  PubMed  CAS  Google Scholar 

  • Lerenius, C., & Mellqvist, M. (2008). Växtskyddsåret 2008. Västergötland, Bohuslän, Dalsland och Värmland. Jönköping: Jordbruksverket.

    Google Scholar 

  • Lerenius, C., Mellqvist, M., Oliver, R. P., & Friesen, T. L. (2010). Växtskyddsåret 2010. Västergötland, Bohuslän, Dalsland och Värmland. Jönköping: Jordbruksverket.

    Google Scholar 

  • Liu, Z. H., Ellwood, S. R., Oliver, R. P., & Friesen, T. L. (2011). Pyrenophora teres: profile of an increasingly damaging barley pathogen. Molecular Plant Pathology, 12(1), 1–19.

    Article  PubMed  Google Scholar 

  • Mathre, D. E. (1997). Compendium of barley diseases (2nd ed.). St. Paul: American Phytopathological Society.

    Google Scholar 

  • Murray, G. M., & Brennan, J. P. (2010). Estimating disease losses to the Australian barley industry. Australasian Plant Pathology, 39, 85–96.

    Article  Google Scholar 

  • Nilsson, H., & Johnsson, L. (1996). Hand-held radiometry of barley infected by stripe disease in a field experiment. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz, 103(5), 517–526.

    Google Scholar 

  • Olofsson, B. (1976). Investigations on Drechslera species in barley and oats. Meddelanden Statens Växtskyddsanstalt, 16, 323–425.

    Google Scholar 

  • Serenius, M., Mironienko, N., & Manninen, O. (2005). Genetic variation, occurrence of mating types and different forms of Pyrenophora teres causing net blotch of barley in Finland. Mycologial Research, 109(7), 809–817.

    Article  CAS  Google Scholar 

  • Smedegård-Petersen, V. (1976). Pathogenesis and genetics of net-spot blotch and leaf stripe of barley caused by Pyrenophora teres and Pyrenophora graminea. (176 pp.) DSR Forlag. Diss. Royal Veterinary and Agricultural University, DK-1871, Copenhagen V., Denmark.

  • SMHI. (2013). SMHI LuftWebb. Swedish Meteorological and Hydrological Institute. http://luftweb.smhi.se. Accessed 2 February 2013.

  • Sperlingsson, K., & Brodal, G. (2011). The osmotic method for detection of Pyrenophora teres and and P. graminea on Hordeum vulgare. Seed Testing International, 141, 34–38.

    Google Scholar 

  • Stefansson, T. S., Serenius, M., & Hallsteinn Hallsson, J. (2012). The genetic diversity of Icelandic populations of two barley leaf pathogens, Rhyncosporium commune and Pyrenophora teres. European Journal of Plant Pathology, 134, 167–180.

    Article  Google Scholar 

  • Steffenson, B. J., Webster, R. K., & Jackson, L. F. (1991). Reduction in yield loss using incomplete resistance to Pyrenophora teres f. teres in barley. Plant Disease, 75, 96–100.

    Article  Google Scholar 

  • Stevens, E. A., Alderson, J., Blakemore, E. J. A., & Reeves, J. C. (1996). Development of a multiplex PCR seed health test to detect and differentiate three pathogens of barley. In G. Marshall (Ed.), Diagnostics in Crop Production symposium proceedings no. 65 (pp. 99–104). Farnham: British Crop Protection Council.

    Google Scholar 

  • Sutton, J. C., & Steele, P. (1983). Effects of seed and foliar fungicides on progress of net blotch and yield in barley. Canadian Journal of Plant Science, 63, 631–639.

    Article  Google Scholar 

  • Svensson, C. (1981). Ny metod för bestämning av utsädesburna sjukdomar. (Nordisk växtskyddskonferans 1981, del 1). Växtskyddsrapporter Jordbruk, 15, 115–118.

    Google Scholar 

  • Thomas, J. E., Taylor, E. J. A., Bates, J. A., & Kenyon, D. M. (2000). Application of a real-time PCR method to detect Pyrenophora species in barley seed, and implications for seed treatment strategies. OEPP/EPPO Bulletin, 30, 555–558.

    Article  Google Scholar 

  • Toubia-Rahme, H., Ali-Haimoud, D. E., Barrault, G., & Albertini, L. (1995). Effect of four fungicides on barley net blotch caused by Drechslera teres. Journal of Phytopathology, 143, 335–339.

    Article  CAS  Google Scholar 

  • Waalwijk, C., Van Der Heide, R., de Vries, I., van der Lee, T., Schoen, C., Costrel-de Corainville, G., et al. (2004). Quantitative detection of Fusarium species in wheat using TaqMan. European Journal of Plant Pathology, 110, 481–494.

    Article  CAS  Google Scholar 

  • Zadoks, J. C., Chang, T. T., & Konzak, C. F. (1974). A decimal code for the growth stages of cereals. Weed Research, 14, 415–421.

    Article  Google Scholar 

Download references

Acknowledgments

This work was funded and supported by the Swedish Farmers’ Foundation of Agricultural Research (SLF), Skånska Lantmännen, and the regional organisations Skåneförsöken and Partnerskap Alnarp. A special thanks to Leona Svobodova at The Crop Research Institute (VURV) Czech Republic for sending Drechslera teres isolates, Karin Sperlingsson at the Swedish Board of Agriculture, Lennart Pålsson at HUSEC, Toma Magyarosi formerly at SW Seed AB, Per Widén at Lantmännen BioAgri, Anders Dahlqvist and PeO Gummesson at ScanBi Diagnostics AB for technical work and valuable discussions.

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  1. Department of Plant Protection Biology, Swedish University of Agricultural Sciences, P.O. Box 102, SE-230 53, Alnarp, Sweden

    Shu-Chin Hysing & Lars Wiik

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  1. Shu-Chin Hysing
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Correspondence to Lars Wiik.

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Hysing, SC., Wiik, L. The role of seed infection level and fungicide seed treatments in control of net blotch in barley. Eur J Plant Pathol 137, 169–180 (2013). https://doi.org/10.1007/s10658-013-0230-7

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