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A dsRNA mycovirus causes hypovirulence of Fusarium graminearum to wheat and maize

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Abstract

The association of Fusarium graminearum isolate China-9 with the dsRNA mycovirus FgV-ch9 was evaluated for hypovirulence-related traits. Single conidia-originating cultures of China-9 isolate can be associated either with high, medium or low amounts of the viral dsRNAs. At high and medium dsRNA levels, China-9 isolates exhibit reduced mycelia growth rate and conidiation capacity, abnormal colony morphology, disorganized cytoplasm, as well as reduced virulence for wheat and maize plants. At low dsRNA levels the fungus shows no symptoms, however, the RNA segments can be detected by RT-PCR. Transfection of the virulent F. graminearum PH-1 isolate with purified Virus-like Particles (VLPs) of FgV-ch9 reduced its conidiation capacity, perithecia formation, and pathogenicity for wheat and maize several folds. These results further demonstrate that FgV-ch9 is associated with hypovirulence of F. graminearum.

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Abbreviations

FgV-ch9:

Fusarium graminearum Virus-china9

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Acknowledgments

We thank E. Wölken for assistance with tissue fixation, sectioning and electron microscopy, and B. Hadeler and C. Krüger for their help with fungal protoplast. OD was supported by the Deutsche Akademische Austausch Dienst (DAAD).

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  1. Omar Darissa

    Present address: Biology Department, Faculty of Science, Bethlehem University, Rue des Freres # 9, Bethlehem, West Bank, Palestine

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  1. Department of Biology, Biocenter Klein Flottbeck, University Hamburg, Ohnhorststr. 18, 22609, Hamburg, Germany

    Günter Adam & Wilhelm Schäfer

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  1. Omar Darissa
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  2. Günter Adam
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  3. Wilhelm Schäfer
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Correspondence to Wilhelm Schäfer.

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Darissa, O., Adam, G. & Schäfer, W. A dsRNA mycovirus causes hypovirulence of Fusarium graminearum to wheat and maize. Eur J Plant Pathol 134, 181–189 (2012). https://doi.org/10.1007/s10658-012-9977-5

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