Amplified fragment length polymorphism (AFLP) based genetic diversity was analyzed for 232 Colletotrichum sublineolum isolates collected between 2002 and 2004 from three geographically distinct regions of Texas, and from Arkansas, Georgia, and Puerto Rico. Results revealed significant levels of polymorphism (59%) among the isolates. Even so, genetic similarity between isolates was high, ranging from 0.78 to 1.00. Clustering of similar isolates did not correlate with either geographic origin or year of collection. Pathotypes of 20 of the isolates were determined using 14 sorghum lines previously used in Brazil and the United States and 4 from Sudan. Seventeen new pathotypes were established from the 18 isolates that gave uniform and consistent reactions on all host differentials over 2 years of greenhouse testing. Differentials BTx378 and QL3 were resistant to all isolates while BTx623 and TAM428 were universally susceptible both years. Each of these lines had shown differential responses in prior studies indicating that the pathogen population has sufficient diversity to adapt rapidly to changes in resistant host lines deployed. When the 2-step pathotype classification scheme was used, the 18 isolates examined in this study were placed in four pathotype groups (A, C, D and G), which would further then be separated into ten distinct pathotypes. Common sets of differentials and a standardized nomenclature will allow for comparison to be made among pathotypes of C. sublineolum detected from different regions and also could help direct planting of appropriate sorghum lines and aid in the development of more durable forms of resistance.
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Abd-Elsalam, K. A., Roshdy, S., Amin, O. E., & Rabani, M. (2010). First morphogenetic identification of the fungal pathogen Colletotrichum musae (Phyllachoraceae) from imported bananas in Saudi Arabia. Genetics and Molecular Research, 9, 2335–2342.
Ali, M. E. K., & Warren, H. L. (1987). Physiological races of Colletotrichum graminicola on sorghum. Plant Disease, 71, 402–404.
Boora, K. S., Frederiksen, R., & Magill, C. (1998). DNA-based markers for a recessive gene conferring anthracnose resistance in sorghum. Crop Science, 38, 1708–1709.
Brown, J. K. M., Andrews, J. H., & Tommerup, I. C. (1995). 5 Pathogens’ responses to the management of disease resistance genes. Advances in Plant Pathology, 11, 75–102.
Cardwell, K. F., Hepperly, P. R., & Frederiksen, R. A. (1989). Pathotypes of Colletotrichum graminicola and seed transmission of sorghum anthracnose. Plant Disease, 73, 255–257.
Casela, C. R., & Ferreira, A. S. (1987). A proposed system of classification of races of Colletotrichum graminicola, causal agent of anthracnose on sorghum (Sorghum bicolor). Fitopatol Bras, 12, 337–344.
Casela, C. R., Ferreira, A. S., & Schaffert, R. E. (1992). Physiological races of Colletotrichum graminicola in Brazil. In W. de Milliano, R. Frederiksen, & G. Bengston (Eds.), Sorghum and millets diseases: a second world review (pp. 209–212). Patancheru: International Crops Research Institute for the Semi-Arid Tropics.
Chala, A., Tronsmo, A. M., & Brurberg, M. B. (2011). Genetic differentiation and gene flow in Colletotrichum sublineolum in Ethiopia, the centre of origin and diversity of sorghum, as revealed by AFLP analysis. Plant Pathology Journal, 60, 474–482.
Erpelding, J. E., & Prom, L. K. (2004). Evaluation of Malian sorghum germplasm for resistance against anthracnose. Plant Pathlogy Journal, 3, 65–71.
Erpelding, J., Prom, L., & Rooney, W. (2005). Variation in anthracnose resistance within the Sudanese sorghum germplasm collection. Plant Genetic Resources Newsletter, 141, 11–14.
Freeman, S., Pham, M., & Rodriguez, R. J. (1993). Molecular genotyping of Colletotrichum species based on arbitrarily primed PCR, A + T-Rich DNA, and nuclear DNA analyses. Experimental Mycology, 17, 309–322.
Freeman, S., & Rodriguez, R. J. (1995). Differentiation of Colletotrichum species responsible for anthracnose of strawberry by arbitrarily primed PCR. Mycological Research, 99, 501–504.
Gale, L. R. (2008). A population genetic approach to variation in Colletotrichum graminicola, the causal agent of sorghum anthracnose. In J. Leslie (Ed.), Sorghum and millets diseases (pp. 191–199). Ames: Iowa State Press.
Guo, J. V., Der Lee, T., Qu, D. Y., Yao, Y. Q., Gong, X. F., Liang, D. L., Xie, K. Y., Wang, X. W., & Govers, F. (2009). Phytophthora infestans isolates from Northern China show high virulence diversity but low genotypic diversity. Plant Biology, 11, 57–67.
Guthrie, P. A. I., Magill, C. W., Frederiksen, R. A., & Odvody, G. (1992). Random amplified polymorphic DNA markers: a system for identifying and differentiating isolates of Colletotrichum graminicola. Phytopathology, 82, 832–835.
Harris, H. B., Johnson, B. J., Dobson, J. W., Jr., & Luttrell, E. S. (1964). Evaluation of anthracnose on grain sorghum. Crop Science, 4, 460–462.
Hulluka, M., & Esele, P. (1992). Sorghum diseases in eastern Africa. In W. de Milliano, R. Frederiksen, & G. Bengston (Eds.), Sorghum and millets diseases: a second world review (pp. 21–24). Patancheru: ICRISAT.
Jaccard, P. (1908). Nouvelles resecherches sur la distribution florale. Bulletin de la Société Vaudoise des sciences naturelles, 44, 223–270.
Jiang, R. H. Y., Tripathy, S., Govers, F., & Tyler, B. M. (2008). RXLR effector reservoir in two Phytophthora species is dominated by a single rapidly evolving superfamily with more than 700 members. Proceedings of the National Academy of Sciences (USA), 105, 4874–4879.
Klein, P. E., Klein, R. R., Cartinhour, S. W., Ulanch, P. E., Dong, J., Obert, J. A., Morishige, D. T., Schlueter, S. D., Childs, K. L., Ale, M., & Mullet, J. E. (2000). A high-throughput AFLP-based method for constructing integrated genetic and physical maps: progress toward a sorghum genome map. Genome Research, 10, 789–807.
Marley, P. S., Thakur, R. P., & Ajayi, O. (2001). Variation among foliar isolates of Colletotrichum sublineolum of sorghum in Nigeria. Field Crops Research, 69, 133–142.
Mathur, K., Thakur, R. P., Neya, A., Marley, P. S., & Casela, C. R. (2002). Sorghum anthracnose-problem and management strategies. In J. Leslie (Ed.), Sorghum and millets diseases (pp. 211–220). Ames: Iowa State Press.
Mehta, P. J., Wiltse, C. C., Rooney, W. L., Collins, S. D., Frederiksen, R. A., Hess, D. E., Chisi, M., & TeBeest, D. O. (2005). Classification and inheritance of genetic resistance to anthracnose in sorghum. Field Crops Research, 93, 1–9.
Menz, M. A., Klein, R. R., Mullet, J. E., Obert, J. A., Unruh, N. C., & Klein, P. E. (2002). A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers. Plant Molecular Biology, 48, 483–499.
Moore, J. W., Ditmore, M., & TeBeest, D. O. (2008). Pathotypes of Colletotrichum sublineolum in Arkansas. Plant Disease, 92, 1415–1420.
Pande, S., Mughogho, L. K., Bandyopadhyay, R., & Karunakar, R. I. (1991). Variation in pathogenicity and cultural characteristics of sorghum isolates of Colletotrichum graminicola in India. Plant Disease, 75, 778–783.
Pastor-Corrales, M. A. (1980). Variation in pathogenicity of Colletotrichum graminicola (Cesati) Wilson and in symptom expression of anthracnose of Sorghum bicolor (L.) Moench. Dissertation. Texas A&M University, 122 pp.
Peever, T.L., Zeigler, R.S., Dorrance, A.E., Correa-Victoria, F.J., & St. Martin, S. (2000). Pathogen population genetics and breeding for disease resistance. On-line feature article for APSNet-Online. doi: 10.1094/APSnetFeature-2000-0700
Prom, L. K., Perumal, R., Erpelding, J. E., Isakeit, T., Montes-Garcia, N., & Magill, C. (2009). A pictorial technique for mass screening of sorghum germplasm for anthracnose (Colletotrichum sublineolum) resistance. The Open Agriculture Journal, 3, 20–25.
Ramasamy, P., Menz, M., Mehta, P., Katilé, S., Gutierrez-Rojas, L., Klein, R., Klein, P., Prom, L., Schlueter, J., Rooney, W., & Magill, C. (2009). Molecular mapping of Cg1, a gene for resistance to anthracnose (Colletotrichum sublineolum) in sorghum. Euphytica, 165, 597–606.
Rohlf, F. J. (2000). NTSYS-pc Numerical Taxonomy and Multivariate Analysis System. New York: Exeter Publications.
Rosewich, U. L., Pettway, R. E., McDonald, B. A., Duncan, R. R., & Frederiksen, R. A. (1998). Genetic structure and temporal dynamics of a Colletotrichum graminicola population in a sorghum disease nursery. Phytopathology, 88, 1087–1093.
Sherriff, C., Whelan, M. J., Arnold, G. M., & Bailey, J. A. (1995). rDNA sequence analysis confirms the distinction between Colletotrichum graminicola and C. sublineolum. Mycological Research, 99, 475–478.
Singh, M., & Boora, K. S. (2008). Molecular characterization of anthracnose resistance gene in sorghum. In G. P. Rao, C. Wagner, R. K. Singh, & M. L. Sharma (Eds.), Plant genomics and bioinformatics (pp. 373–388). Houston: Studium Press LLC.
Sneath, P., & Sokal, R. (1973). Numerical taxonomy. The principles and practices of classification. San Francisco: W.H. Freeman and Co.
Sutton, B. (1980). The coelomycetes: fungi imperfecti with pycnidia, acervuli and stromata. Kew: Commonwealth Mycological Institute.
Suzuki, T., Tanaka-Miwa, C., Ebihara, Y., Ito, Y., & Uematsu, S. (2007). Genetic polymorphism and virulence of Colletotrichum gloeosporioides isolated from strawberry (Fragaria × ananassa Duchesne). Journal of General Plant Pathology, 76, 247–253.
Thomas, M. D., Sissoko, I., & Sacco, M. (1995). Development of leaf anthracnose and its effect on yield and grain weight of sorghum in West Africa. Plant Dsease, 80, 151–153.
Thuan, N., Bigirimana, J., Roumen, E. V., Der Straeten, D., & Höfte, M. (2006). Molecular and pathotype analysis of the rice blast fungus in North Vietnam. European Journal of Plant Pathology, 114, 381–396.
Vaillancourt, L. J., & Hanau, R. M. (1992). Genetic and morphological comparisons of Glomerella (Colletotrichum) isolates from maize and from sorghum. Experimental Mycology, 16, 219–229.
Valèrio, H., Rèsende, M., Weikert-Oliveira, R., & Casela, C. (2005). Virulence and molecular diversity in Colletotrichum graminicola from Brazil. Mycopathologia, 159, 449–459.
Waniska, R. D., Venkatesha, R. T., Chandrashekar, A., Krishnaveni, S., Bejosano, F. P., Jeoung, J., Jayaraj, J., Muthukrishnan, S., & Liang, G. H. (2001). Antifungal proteins and other mechanisms in the control of sorghum stalk rot and grain mold. Journal of Agricultural and Food Chemistry, 49, 4732–4742.
Warren, H. L. (1986). Leaf anthracnose. In R. A. Frederiksen (Ed.), Compendium of sorghum diseases (pp. 10–11). St. Paul: The American Phytopathological Society.
Support from Texas Agrilife Research and the USDA is gratefully acknowledged. This paper is Contribution No. 11-247-J from the Kansas Agricultural Experiment Station, Manhattan.
L. K. Prom and R. Perumal have equal contributions.
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Prom, L.K., Perumal, R., Erattaimuthu, S.R. et al. Genetic diversity and pathotype determination of Colletotrichum sublineolum isolates causing anthracnose in sorghum. Eur J Plant Pathol 133, 671–685 (2012). https://doi.org/10.1007/s10658-012-9946-z
- Fungal plant pathogen
- Host differentials