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Avirulent strain of Colletotrichum induces a systemic resistance in strawberry

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Abstract

Strawberry plants exposed to an avirulent isolate of Colletotrichum fragariae acquired strong resistance against a virulent strain of C. acutatum. Biochemical, morphological and molecular markers indicated that the strong defence response was associated with an oxidative burst and a transient accumulation of salicylic acid (SA). A maximum accumulation of H2O2 and O2 − was observed 8 h after inoculation (hai), callose was detected 48 hai, and a peak of SA was observed 48 hai. Biochemical and phytopathogenic analyses carried out in non-treated tissues revealed that the defence response was systemic and remained fully active 60 days after the first inoculation. Experiments also showed that the resistance acquired by mother plants after the inoculation with the avirulent isolate could be passed to daughter plants through runners. Further characterization of the induced systemic resistance showed that the resistance was not only effective against a virulent strain of C. acutatum but also against Botrytis cinerea.

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Abbreviations

dai:

Days after inoculation

DSR:

Disease severity rating

hai:

Hours after inoculation

ROS:

Reactive oxygen species

SA:

Salicylic acid

SAR:

Systemic acquired resistance

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Acknowledgments

This work was partially supported by the Consejo de Investigaciones de la Universidad Nacional de Tucumán (CIUNT, 26/D423); Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Agencia Nacional de Promoción Científica y Tecnológica (Préstamo BID PICT 2008–2105). APC and JDR are members of CONICET. SMS is member of INTA; NRCh and CFG are Fellows of CONICET. APC is also member of EEAOC of Tucumán. We are also grateful to Dr. Cecilia Díaz (FAZ, UNT) for data analysis.

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Authors and Affiliations

  1. Instituto Superior de Investigaciones Biológicas (INSIBIO; CONICET-UNT) and Instituto de Química Biológica “Dr. Bernabe’ Bloj”, Universidad Nacional de Tucumán, Chacabuco 461, 4000, San Miguel de Tucumán, Argentina

    S. M. Salazar, C. F. Grellet, N. R. Chalfoun, A. P. Castagnaro & J. C. Díaz Ricci

  2. Sección Biotecnología de la Estación Experimental Agroindustrial Obispo Colombres (EEAOC)-Unidad Asociada al INSIBIO, Av. William Cross 3150, Las Talitas, 4101, Tucumán, Argentina

    A. P. Castagnaro

Authors
  1. S. M. Salazar
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  2. C. F. Grellet
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  3. N. R. Chalfoun
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  4. A. P. Castagnaro
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  5. J. C. Díaz Ricci
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Corresponding authors

Correspondence to A. P. Castagnaro or J. C. Díaz Ricci.

Additional information

This paper is part of the first author’s doctoral thesis.

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Salazar, S.M., Grellet, C.F., Chalfoun, N.R. et al. Avirulent strain of Colletotrichum induces a systemic resistance in strawberry. Eur J Plant Pathol 135, 877–888 (2013). https://doi.org/10.1007/s10658-012-0134-y

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  • DOI: https://doi.org/10.1007/s10658-012-0134-y

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