Resistance to blast (Magnaporthe grisea) in a mini-core collection of finger millet germplasm
Blast caused by Pyricularia grisea [teleomorph: Magnaporthe grisea] is an economically important and widespread disease of finger millet in the world. Host resistance is the most economical and effective means of combating this disease as finger millet is predominantly grown by resource-poor and marginal farmers. At the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), we evaluated a finger millet mini-core collection of 80 germplasm accessions (about 1 % of the total germplasm collection representing major trait variability) for blast resistance both in the field and greenhouse. Field evaluation was done using a refined screening technique that included new improved rating scales for leaf, neck and finger infection. Sixty six of the 80 accessions showed combined resistance to leaf, neck and finger blast in two seasons (2009 and 2010) of field screening. A highly significant and positive correlation was found between neck and finger blast ratings (r = 0.92), whereas small but significant correlations were found between leaf blast and neck blast (r = 0.25) and between leaf blast and finger blast (r = 0.30). These accessions were also screened for leaf blast resistance in the greenhouse by artificial inoculation of seedlings to confirm field observations. Fifty-eight of the 80 accessions were resistant to leaf blast in the greenhouse screen as well. These resistant accessions represented one wild (africana) and four cultivated races (vulgaris, plana, elongate and compacta) of finger millet that originated from 13 countries in Asia and Africa and exhibited considerable diversity for agronomic traits, such as maturity period, plant height and panicle type. These blast resistant accessions from the mini-core collection would be useful in finger millet disease resistance breeding programs.
KeywordsBlast disease Finger millet Host plant resistance Mini-core collection and rating scales
The authors gratefully acknowledge the financial support from the BMZ/GTZ project on “Sustainable conservation and utilization of genetic resources of two underutilized crops-finger millet and foxtail millet-to enhance productivity, nutrition and income in Africa and Asia” funded by the Federal Ministry for Economic Cooperation and Development (BMZ), Germany to carry out this activity. The authors are also grateful to Mr. V. P. Rao, Cereals pathology, ICRISAT for his support in field and greenhouse experiments.
- Esele, J. P. E. (2002). Disease of finger millet: A global review. In J. F. Leslie (Ed.), Sorghum and millets diseases. Iowa: Iowa State Press.Google Scholar
- FAO (2004). Food and Agriculture Organization of the United Nations, Rome. http://faostat.fao.org.
- Gowda, B. T. S., Seetharam, A., Vishwanath, S., & Sannegowda, S. (1986). Incorporation of blast resistance to Indian elite finger millet cultivars from African cultivar IE 1012. SABRO Journal, 18, 119–120.Google Scholar
- Koutroubas, D. S., Katsantonis, D., Ntanos, D. A., & Lupatto, E. (2009). Blast fungus inoculation reduces accumulation and remobilization of pre-anthesis assimilates to rice grains. Phytopathologia Mediterranea, 48, 240–252.Google Scholar
- Mgonja, M. A., Lenne, J. M., Manyasa, E., & Sreenivasaprasad, S. (Eds.) (2007). Finger millet blast management in East Africa: creating opportunities for improving production and utilization of finger millet: proceedings of the first International finger millet stakeholder workshop, Nairobi (pp. 1–192). (International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, AP, India) ISBN: 978-92-9066-505-2.Google Scholar
- Nagaraja, A., Jagadish, P. S., Ashok, E. G., & Krishne Gowda, K. T. (2007). Avoidance of finger millet blast by ideal sowing time and assessment of varietal performance under rainfed production situations in Karnataka. Journal of Mycopathological Research, 45(2), 237–240.Google Scholar
- Nagaraja, A., Nanja Reddy, Y. A., Anjaneya Reddy, B., Patro, T. S. S. K., Kumar, B., Kumar, J., & Krishne Gowda, K. T. (2010). Reaction of finger millet recombinant inbred lines (RILs) to blast. Crop Research (Hissar), 39(1, 2&3), 120–122.Google Scholar
- National Research Council. (1996). Finger millet. In F. R. Ruskin (Ed.), Lost crops of Africa: Grains (pp. 39–58). Washington, DC: National Academy Press.Google Scholar
- Ou, S. H. (1985). Rice diseases (2nd ed.). Kew: Commonwealth Mycological Institute.Google Scholar
- Pande, S., Mukuru, S. Z., King, S. B., & Karunakar, R. I. (1995). Biology of and resistance to finger millet blast in Kenya and Uganda. In S. Z. Mukuru & S. B. King (Eds.), Proceedings of the eighth EARSAM regional workshop on sorghum and millets, 30 Oct–5 Nov 1992, Sudan (pp. 83–92). Andhra Pradesh: ICRISAT.Google Scholar
- Prasada Rao, K. E., & deWet, J. M. G. (1997). Small millets. In F. Fuccillo, L. Sears, & P. Stableton (Eds.), Biodiversity in trust: Conservation and use of plant genetic resources in CGIAR centers (pp. 259–272). United Kingdom: Cambridge University Press.Google Scholar
- Rachie, O. K., & Peters, V. L. (1977). Tahe Eleusines: A review of the world literature. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, AP, India.Google Scholar
- Rao, A. N. S. (1990). Estimates of losses in finger millet (Eleusine coracana) due to blast disease (Pyricularia grisea). Journal of Agricultural Sciences, 24, 57–60.Google Scholar
- Sharma, R., Upadhyaya, H. D., Manjunatha, S. V., Rao, V. P., & Thakur, R. P. (2012a). Resistance to foliar diseases in a mini-core collection of sorghum germplasm. Plant Disease (In press) http://dx.doi.org/10.1094/PDIS-10-11-0875-RE.
- Sharma, M., Rathore, A., Naga Mangala, U., Ghosh, R., Sharma, S., Upadhyaya, H. D., & Pande, S. (2012b). New sources of resistance to fusarium wilt and sterility mosaic disease in a mini-core collection of pigeonpea germplasm. European Journal of Plant Pathology, 133(3), 707–714.CrossRefGoogle Scholar
- Somashekhara, Y. M., Viswanath, S., & Anilkumar, T. B. (1991). Evaluation of finger millet (Eleusine coracana (L.) Gaertn) cultivars for their reactions to blast (Pyricularia grisea Sacc). Tropical Agriculture, 68, 231–234.Google Scholar
- Takan, J. P., Akello, B., Esele, P., Manyasa, E. O., Obilana, A. B., & Audi, P. O. (2004). Finger millet blast pathogen diversity and management in east Africa: a summary of project activities and outputs. International Sorghum and Millets Newsletter, 45, 66–69.Google Scholar
- Takan, J. P., Chipili, J., Muthumeenakshi, S., Talbot, N. J., Manyasa, E. O., Bandyopadhyay, R., Sere, Y., Nutsugah, S. K., Talhinhas, P., Hossain, M., Brown, A. E., & Sreenivasaprasad, S. (2012). Magnaporthe oryzae populations adapted to finger millet and rice exhibit distinctive patterns of genetic diversity, sexuality and host interaction. Molecular Biotechnology, 50(2), 145–158.PubMedCrossRefGoogle Scholar
- Thakur, R. P., Sharma, R., Rai, K. N., Gupta, S. K., & Rao, V. P. (2009). Screening techniques and resistance sources for foliar blast in pearl millet. Journal of SAT Agricultural Research, 7, 1–5.Google Scholar
- Upadhyaya, H. D., Pundir, R. P. S., & Gowda, C. L. L. (2007). Genetic resources diversity of finger millet—A global perspective. In: M. A. Mgonja., J. M. Lenne., E. Manyasa & S. Sreenivasaprasad. Finger Millet Blast Management in East Africa. Creating opportunities for improving production and utilization of finger millet (pp. 90–101). International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, AP, India.Google Scholar
- Upadhyaya, H. D., Sarma, N. D. R. K., Ravishankar, C. R., Albrecht, T., Narasimhudu, Y., Singh, S. K., Varshney, R. K., Reddy, V. G., Singh, S., Dwivedi, S. L., Wanyera, N., Oduori, C. O. A., Mgonja, M. A., Kisandu, D. B., Parzies, H. K., & Gowda, C. L. L. (2010). Developing mini-core collection in finger millet using multilocation data. Crop Science, 50, 1924–1931.CrossRefGoogle Scholar
- Upadhyaya, H. D., Ramesh, S., Sharma, S., Singh, S. K., Varshney, S. K., Sarma, N. D. R. K., Ravishankar, C. R., Narasimhudu, Y., Reddy, V. G., Sahrawat, K. L., Dhanalakshmi, T. N., Mgonja, M. A., Parzies, H. K., Gowda, C. L. L., & Singh, S. (2011). Genetic diversity for grain nutrients contents in a core collection of finger millet (Eleusine coracana (L.) Gaertn.) germplasm. Field Crops Research, 121, 42–52.CrossRefGoogle Scholar
- Vadez, V., Krishnamurthy, L., Serraj, R., Gaur, P. M., Upadhyaya, H. D., Hoisington, D., Varshney, R. K., Turner, N. C., & Siddique, K. H. M. (2007). Large variation in salinity tolerance in chickpea is explained by differences in sensitivity at reproductive stage. Field Crops Research, 104, 123–129.CrossRefGoogle Scholar
- Vishwanath, S., Sanne Gowda, S., Seetharam, A., & Shankare Gowda, B. T. (1986). Reaction to blast disease of released and pre-released varieties of finger millet from different states. Millet Newsletter, 5, 31.Google Scholar