European Journal of Plant Pathology

, Volume 135, Issue 2, pp 299–311 | Cite as

Resistance to blast (Magnaporthe grisea) in a mini-core collection of finger millet germplasm

  • T. Kiran Babu
  • R. P. Thakur
  • H. D. Upadhyaya
  • P. N. Reddy
  • R. Sharma
  • A. G. Girish
  • N. D. R. K. Sarma
Article

Abstract

Blast caused by Pyricularia grisea [teleomorph: Magnaporthe grisea] is an economically important and widespread disease of finger millet in the world. Host resistance is the most economical and effective means of combating this disease as finger millet is predominantly grown by resource-poor and marginal farmers. At the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), we evaluated a finger millet mini-core collection of 80 germplasm accessions (about 1 % of the total germplasm collection representing major trait variability) for blast resistance both in the field and greenhouse. Field evaluation was done using a refined screening technique that included new improved rating scales for leaf, neck and finger infection. Sixty six of the 80 accessions showed combined resistance to leaf, neck and finger blast in two seasons (2009 and 2010) of field screening. A highly significant and positive correlation was found between neck and finger blast ratings (r = 0.92), whereas small but significant correlations were found between leaf blast and neck blast (r = 0.25) and between leaf blast and finger blast (r = 0.30). These accessions were also screened for leaf blast resistance in the greenhouse by artificial inoculation of seedlings to confirm field observations. Fifty-eight of the 80 accessions were resistant to leaf blast in the greenhouse screen as well. These resistant accessions represented one wild (africana) and four cultivated races (vulgaris, plana, elongate and compacta) of finger millet that originated from 13 countries in Asia and Africa and exhibited considerable diversity for agronomic traits, such as maturity period, plant height and panicle type. These blast resistant accessions from the mini-core collection would be useful in finger millet disease resistance breeding programs.

Keywords

Blast disease Finger millet Host plant resistance Mini-core collection and rating scales 

References

  1. Esele, J. P. E. (2002). Disease of finger millet: A global review. In J. F. Leslie (Ed.), Sorghum and millets diseases. Iowa: Iowa State Press.Google Scholar
  2. FAO (2004). Food and Agriculture Organization of the United Nations, Rome. http://faostat.fao.org.
  3. Gowda, B. T. S., Seetharam, A., Vishwanath, S., & Sannegowda, S. (1986). Incorporation of blast resistance to Indian elite finger millet cultivars from African cultivar IE 1012. SABRO Journal, 18, 119–120.Google Scholar
  4. IBPGR. (1985). Descriptors for finger millet. Rome: International Board for Plant Genetic Resources Secretariat. http://www.bioversityinternational.org.Google Scholar
  5. Kashiwagi, J., Krishnamurthy, L., Upadhyaya, H. D., Krishna, H., Chandra, S., Vadez, V., & Serraj, R. (2005). Genetic variability of drought avoidance root traits in the mini core germplasm collection of chickpea (Cicer arietinum L.). Euphytica, 146, 213–222.CrossRefGoogle Scholar
  6. Koutroubas, D. S., Katsantonis, D., Ntanos, D. A., & Lupatto, E. (2009). Blast fungus inoculation reduces accumulation and remobilization of pre-anthesis assimilates to rice grains. Phytopathologia Mediterranea, 48, 240–252.Google Scholar
  7. Krishnamurthy, L., Kashiwagi, J., Gaur, P. M., Upadhyaya, H. D., & Vadez, V. (2010). Sources of tolerance to terminal drought in the chickpea (Cicer arietinum L.) mini-core germplasm. Field Crops Research, 119, 322–330.CrossRefGoogle Scholar
  8. Malleshi, N. G., & Klopfenstein, C. F. (1998). Nutrient composition, amino acid and vitamin contents of malted sorghum, pearl millet, finger millet and their rootlets. International Journal of Food Science & Nutrition, 49, 415–422.CrossRefGoogle Scholar
  9. Mgonja, M. A., Lenne, J. M., Manyasa, E., & Sreenivasaprasad, S. (Eds.) (2007). Finger millet blast management in East Africa: creating opportunities for improving production and utilization of finger millet: proceedings of the first International finger millet stakeholder workshop, Nairobi (pp. 1–192). (International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, AP, India) ISBN: 978-92-9066-505-2.Google Scholar
  10. Nagaraja, A., Jagadish, P. S., Ashok, E. G., & Krishne Gowda, K. T. (2007). Avoidance of finger millet blast by ideal sowing time and assessment of varietal performance under rainfed production situations in Karnataka. Journal of Mycopathological Research, 45(2), 237–240.Google Scholar
  11. Nagaraja, A., Nanja Reddy, Y. A., Anjaneya Reddy, B., Patro, T. S. S. K., Kumar, B., Kumar, J., & Krishne Gowda, K. T. (2010). Reaction of finger millet recombinant inbred lines (RILs) to blast. Crop Research (Hissar), 39(1, 2&3), 120–122.Google Scholar
  12. National Research Council. (1996). Finger millet. In F. R. Ruskin (Ed.), Lost crops of Africa: Grains (pp. 39–58). Washington, DC: National Academy Press.Google Scholar
  13. Ou, S. H. (1985). Rice diseases (2nd ed.). Kew: Commonwealth Mycological Institute.Google Scholar
  14. Pande, S., Mukuru, S. Z., King, S. B., & Karunakar, R. I. (1995). Biology of and resistance to finger millet blast in Kenya and Uganda. In S. Z. Mukuru & S. B. King (Eds.), Proceedings of the eighth EARSAM regional workshop on sorghum and millets, 30 Oct–5 Nov 1992, Sudan (pp. 83–92). Andhra Pradesh: ICRISAT.Google Scholar
  15. Pande, S., Kishore, G. K., Upadhyaya, H. D., & Rao, J. N. (2006). Identification of sources of multiple disease resistance in mini-core collection of chickpea. Plant Disease, 90, 1214–1218.CrossRefGoogle Scholar
  16. Prasada Rao, K. E., & deWet, J. M. G. (1997). Small millets. In F. Fuccillo, L. Sears, & P. Stableton (Eds.), Biodiversity in trust: Conservation and use of plant genetic resources in CGIAR centers (pp. 259–272). United Kingdom: Cambridge University Press.Google Scholar
  17. Rachie, O. K., & Peters, V. L. (1977). Tahe Eleusines: A review of the world literature. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, AP, India.Google Scholar
  18. Rao, A. N. S. (1990). Estimates of losses in finger millet (Eleusine coracana) due to blast disease (Pyricularia grisea). Journal of Agricultural Sciences, 24, 57–60.Google Scholar
  19. Sharma, R., Rao, V. P., Upadhyaya, H. D., Reddy, V. G., & Thakur, R. P. (2010). Resistance to grain mold and downy mildew in mini-core collection of sorghum germplasm. Plant Disease, 94, 439–444.CrossRefGoogle Scholar
  20. Sharma, R., Upadhyaya, H. D., Manjunatha, S. V., Rao, V. P., & Thakur, R. P. (2012a). Resistance to foliar diseases in a mini-core collection of sorghum germplasm. Plant Disease (In press) http://dx.doi.org/10.1094/PDIS-10-11-0875-RE.
  21. Sharma, M., Rathore, A., Naga Mangala, U., Ghosh, R., Sharma, S., Upadhyaya, H. D., & Pande, S. (2012b). New sources of resistance to fusarium wilt and sterility mosaic disease in a mini-core collection of pigeonpea germplasm. European Journal of Plant Pathology, 133(3), 707–714.CrossRefGoogle Scholar
  22. Somashekhara, Y. M., Viswanath, S., & Anilkumar, T. B. (1991). Evaluation of finger millet (Eleusine coracana (L.) Gaertn) cultivars for their reactions to blast (Pyricularia grisea Sacc). Tropical Agriculture, 68, 231–234.Google Scholar
  23. Takan, J. P., Akello, B., Esele, P., Manyasa, E. O., Obilana, A. B., & Audi, P. O. (2004). Finger millet blast pathogen diversity and management in east Africa: a summary of project activities and outputs. International Sorghum and Millets Newsletter, 45, 66–69.Google Scholar
  24. Takan, J. P., Chipili, J., Muthumeenakshi, S., Talbot, N. J., Manyasa, E. O., Bandyopadhyay, R., Sere, Y., Nutsugah, S. K., Talhinhas, P., Hossain, M., Brown, A. E., & Sreenivasaprasad, S. (2012). Magnaporthe oryzae populations adapted to finger millet and rice exhibit distinctive patterns of genetic diversity, sexuality and host interaction. Molecular Biotechnology, 50(2), 145–158.PubMedCrossRefGoogle Scholar
  25. Thakur, R. P., Sharma, R., Rai, K. N., Gupta, S. K., & Rao, V. P. (2009). Screening techniques and resistance sources for foliar blast in pearl millet. Journal of SAT Agricultural Research, 7, 1–5.Google Scholar
  26. Torres, C. Q., & Teng, P. S. (1993). Path-coefficient and regression analysis of the effects of leaf and panicle blast on tropical rice yield. Crop Protection, 12, 296–302.CrossRefGoogle Scholar
  27. Upadhyaya, H. D., & Ortiz, R. (2001). A mini-core subset for capturing diversity and promoting utilization of chickpea genetic resources in crop improvement. Theoretical and Applied Genetics, 102, 1292–1298.CrossRefGoogle Scholar
  28. Upadhyaya, H. D., Gowda, C. L. L., Pundir, R. P. S., Reddy, V. G., & Singh, S. (2006). Development of core subset of finger millet germplasm using geographical origin and data on 14 quantitative traits. Genetic Resources and Crop Evolution, 53, 679–685.CrossRefGoogle Scholar
  29. Upadhyaya, H. D., Pundir, R. P. S., & Gowda, C. L. L. (2007). Genetic resources diversity of finger millet—A global perspective. In: M. A. Mgonja., J. M. Lenne., E. Manyasa & S. Sreenivasaprasad. Finger Millet Blast Management in East Africa. Creating opportunities for improving production and utilization of finger millet (pp. 90–101). International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, AP, India.Google Scholar
  30. Upadhyaya, H. D., Sarma, N. D. R. K., Ravishankar, C. R., Albrecht, T., Narasimhudu, Y., Singh, S. K., Varshney, R. K., Reddy, V. G., Singh, S., Dwivedi, S. L., Wanyera, N., Oduori, C. O. A., Mgonja, M. A., Kisandu, D. B., Parzies, H. K., & Gowda, C. L. L. (2010). Developing mini-core collection in finger millet using multilocation data. Crop Science, 50, 1924–1931.CrossRefGoogle Scholar
  31. Upadhyaya, H. D., Ramesh, S., Sharma, S., Singh, S. K., Varshney, S. K., Sarma, N. D. R. K., Ravishankar, C. R., Narasimhudu, Y., Reddy, V. G., Sahrawat, K. L., Dhanalakshmi, T. N., Mgonja, M. A., Parzies, H. K., Gowda, C. L. L., & Singh, S. (2011). Genetic diversity for grain nutrients contents in a core collection of finger millet (Eleusine coracana (L.) Gaertn.) germplasm. Field Crops Research, 121, 42–52.CrossRefGoogle Scholar
  32. Vadez, V., Krishnamurthy, L., Serraj, R., Gaur, P. M., Upadhyaya, H. D., Hoisington, D., Varshney, R. K., Turner, N. C., & Siddique, K. H. M. (2007). Large variation in salinity tolerance in chickpea is explained by differences in sensitivity at reproductive stage. Field Crops Research, 104, 123–129.CrossRefGoogle Scholar
  33. Vishwanath, S., Sanne Gowda, S., Seetharam, A., & Shankare Gowda, B. T. (1986). Reaction to blast disease of released and pre-released varieties of finger millet from different states. Millet Newsletter, 5, 31.Google Scholar
  34. Wald, A. (1943). Test of statistical hypothesis concerning several parameters when the number of observation is large. Transactions of the American Mathematical Society, 54, 426–482.CrossRefGoogle Scholar
  35. Wu, J. L., Sinha, P. K., Variar, M., Zheng, K. L., Leach, J. E., Courtois, B., & Leaung, H. (2004). Association between molecular markers and blast resistance in an advanced backcross population of rice. Theoretical and Applied Genetics, 108, 1024–1032.PubMedCrossRefGoogle Scholar

Copyright information

© KNPV 2012

Authors and Affiliations

  • T. Kiran Babu
    • 1
  • R. P. Thakur
    • 2
  • H. D. Upadhyaya
    • 2
  • P. N. Reddy
    • 1
  • R. Sharma
    • 2
  • A. G. Girish
    • 2
  • N. D. R. K. Sarma
    • 1
  1. 1.Acharya N. G. Ranga Agricultural University (ANGRAU)HyderabadIndia
  2. 2.International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)HyderabadIndia

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