Abstract
Moniliophthora perniciosa is a fungus that causes witches’ broom disease (WBD) in the cacao tree (Theobroma cacao). The M. perniciosa genome contains different transposable elements; this prompted an evaluation of the use of its retrotransposons as molecular markers for population studies. The inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) techniques were used to study the variability of 70 M. perniciosa isolates from different geographic origins and biotypes. A total of 43 loci was amplified. Cluster analysis of different geographical regions of C biotype revealed two large groups in the state of Bahia, Brazil. Techniques using retrotransposon-based molecular markers showed advantages over previously used molecular techniques for the study of genetic variability in M. perniciosa.
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We are grateful to Brazilian agencies CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior).
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Santana, M.F., de Araújo, E.F., de Souza, J.T. et al. Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa . Eur J Plant Pathol 134, 497–507 (2012). https://doi.org/10.1007/s10658-012-0031-4
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DOI: https://doi.org/10.1007/s10658-012-0031-4