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Differential induction of pathogenesis-related proteins in banana in response to Mycosphaerella fijiensis infection

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Abstract

Black leaf streak or “black Sigatoka” is one of the most important diseases affecting bananas and plantains worldwide. Very few studies have been published on the host-pathogen interaction of this pathosystem, particularly at the molecular level. The aim of this work was to analyze, under controlled conditions, the enzyme activity of peroxidase (POX), phenylalanine ammonia lyase (PAL), β-1, 3-glucanase (GLU) and chitinase (CHI) as well as the production of H2O2 in banana plants infected with Mycosphaerella fijiensis. Defence responses were examined and compared in a resistant (Calcutta 4) and a susceptible (Williams) cultivar. Plants were inoculated and tested for enzyme activity at 0, 6, 12, 18, 24, 48 and 72 h after infection (HAI) and 6, 9, 12, 15 and 18 days after inoculation (DAI). A rapid induction of PAL, POX and GLU was observed in the resistant cultivar at 6–18 HAI as well as H2O2 production at 72 HAI. In contrast, in the susceptible cultivar, induction of these enzymes was only observed from 6 DAI. These results suggest that the first 72 HAI are important in determining the response of the host to the disease. Further studies characterizing banana responses to M. fijiensis at the early stages of the infection are necessary in order to better understand this host-pathogen interaction.

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Acknowledgments

This work was supported by the Instituto para el desarrollo de la Ciencia y la Tecnología “Francisco José de Caldas” (Colciencias), Colombia, grants No. 2213-12-17825 and 2213-452-21253; Corporación para Investigaciones Biológicas (CIB) and Dirección de Investigaciones Medellín (DIME) of the Universidad Nacional de Colombia sede Medellín. The authors of this article wish to thank Professor Jairo Castaño from Universidad de Caldas for the Calcutta 4 plantlets used in this work.

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Authors and Affiliations

  1. Plant Biotechnology Unit UNALMED-CIB Corporación Para Investigaciones Biológicas – CIB, Cra. 72 No 78 B 141, Medellín, Colombia

    J. M. Torres, H. Calderón, E. Rodríguez-Arango & R. Arango

  2. Escuela de Biociencias, Universidad Nacional de Colombia, Calle 59A No 63-20 - Núcleo El Volador, Medellín, Colombia

    R. Arango

  3. Departamento de Ciencias Agronómicas, Universidad Nacional de Colombia sede, Medellín, Colombia

    J. G. Morales

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  1. J. M. Torres
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  2. H. Calderón
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  3. E. Rodríguez-Arango
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  5. R. Arango
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Correspondence to R. Arango.

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Torres, J.M., Calderón, H., Rodríguez-Arango, E. et al. Differential induction of pathogenesis-related proteins in banana in response to Mycosphaerella fijiensis infection. Eur J Plant Pathol 133, 887–898 (2012). https://doi.org/10.1007/s10658-012-0012-7

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