Abstract
In China, wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most destructive diseases of wheat. The Longnan and Linxia regions in Gansu Province and Qinghai Province are the major over-summering regions for the pathogen and key epidemiological zones in Northwest China. Population genetic diversity and interregional long-distance spread of the wheat stripe rust pathogen in Northwest China were studied using SSR markers. The genetic diversity in the Longnan population was much higher than those in the Linxia and Qinghai populations. Therefore, the molecular data confirmed that the Longnan region is a center of genetic diversity for P. striiformis f. sp. tritici in Northwest China. The low genetic differentiation (Gst = 0.15) and the extensive gene flow (Nm = 1.37) were found among the three regions in Northwest China. The most important conclusion of this study is that the stripe rust inoculum in Qinghai can come from both Longnan and Linxia, but mainly from Longnan directly in the spring.
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References
Chen, X. M. (2005). Epidemiology and control of stripe rust [Puccinia striiformis f.sp tritici] on wheat. Canada Journal Plant Pathology, 27, 314–337.
Chen, C. Q., Zheng, W. M., Huang, L. L., Lu, N. H., & Kang, Z. S. (2008). Isolation of microsatellite loci from expressed sequence tag library of Puccinia striiformis f. sp. tritici. Molecular Ecology Resource, 9, 236–238.
Enjalbert, J., Duan, X., Giraud, T., Vautrin, D., de Vallavieille-Pope, C., & Solignac, M. (2002). Isolation of twelve microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia striiformis f. sp. tritici. Molecular Ecology Notes, 2, 563–565.
Excoffier, L., Smouse, P. E., & Quattro, J. M. (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics, 131, 479–491.
Felsenstein, J. (1989). Phylip: phylogeny inference package (version 3.2). Cladistics, 5, 164–166.
Li, Z. Q., & Zeng, S. M. (2000). Wheat rusts in China (pp. 1–112). Beijing: China Agricultural.
Li, Z. Q., Wang, M. N., Jia, M. G., Lu, H. L., Shang, H. S., & Kang, Z. S. (1997). Study on the epidemiology and control strategy of wheat stripe rust in South Gansu. Acta University Agriculture Boreali-Occidentalia, 25, 1–5.
Line, R. F. (2002). Stripe rust of wheat and barley in North America, A retrospective historical review. Annual Review of Phytopathology, 40, 75–118.
Lorys, M. M. A., Christian, L., de Vallavieille-Pope, C., & Neema, C. (2002). Genetic variability in Puccinia striiformis f. sp. tritici populations sampled on a local scale during natural epidemics. Applied and Environmental Microbiology, 12, 6138–6145.
Mboup, M., Leconte, M., Gautier, A., Wan, A. M., Chen, W., de Vallavieille-Pope, C., et al. (2009). Evidence of genetic recombination in wheat yellow rust populations of a Chinese oversummering area. Fungal Genetics and Biology, 46, 299–307.
Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the USA, 70, 3321–3323.
Schneider, S., Roessli, D., & Excoffier, L. (2000). Arlequin ver. 2000: a software for population genetics data analysis. Genetics and Biometry Laboratory, University of Geneva.
Shan, W., Chen, S., Kang, Z., Wu, L., & Li, Z. (1998). Genetic diversity in Puccinia striiformis Westend. f. sp. tritici revealed by pathogen genome-specific repetitive sequence. Canadian Journal of Botany, 76, 587–595.
Stubbs, R. W. (1985). Stripe rust. In A. P. Roelfs & W. R. Bushnell (Eds.), The cereal rusts, vol. 2, diseases, distribution, epidemiology, and control (pp. 61–101). Orlando: Academic.
Wan, A. M., Zhao, Z. H., Chen, X. M., & He, Z. H. (2004). Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Disease, 88, 896–904.
Wan, A. M., Chen, X. M., & He, Z. H. (2007). Wheat stripe rust in China. Australian Journal of Agricultural Research, 6, 605–619.
Yeh, F. C., Yang, R. C., & Boyle, T. (1997). POPGENE.CIFOR and University of Alberta, Canada, Version 1.21.
Zhang, F. M., & Ge, S. (2002). Data analysis in population genetics. I. analysis of RAPD data with AMOVA. Biodiversity Science, 10, 438–444.
Zheng, W. M., Liu, F., & Kang, Z. S. (2001). AFLP fingerprinting of Chinese epidemic strains of Puccinia striiformis f. sp. tritici. Progress in Nature Science, 11, 586–593.
Acknowledgements
This study was supported by grants from National Basic Research Program of China (No. 2006CB100203), Nature Science Foundation of China (No. 30930064), the Hangye Program from the Ministry of Agriculture of China (200903035-02), and the 111 Project from the Ministry of Education of China (B07049).
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Lu, N., Wang, J., Chen, X. et al. Spatial genetic diversity and interregional spread of Puccinia striiformis f. sp. tritici in Northwest China. Eur J Plant Pathol 131, 685–693 (2011). https://doi.org/10.1007/s10658-011-9842-y
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DOI: https://doi.org/10.1007/s10658-011-9842-y