Abstract
Vascular puncture inoculation (VPI) of plant viruses previously has been conducted either manually or by use of a commercial engraving tool and laboratory-fabricated needle arrays. In an effort to improve this technique, a linear-motion tattoo machine driving industry-standard needle arrays was tested as a means of delivering plant viruses into maize and small grain seed embryos. The new method was applied in the successful transmission of maize rayado fino virus (MRFV), the type member of the genus Marafivirus, from an archived sample to maize. Subsequent transfer of MRFV from the sap of an infected plant using the method produced an average infection rate in maize of 70% (range 39–93%). Maize, oat, and triticale were successfully infected with oat blue dwarf virus (OBDV) using the method; similar infection rates were observed between maize seeds inoculated with the tattoo machine and those inoculated with the engraving machine when using prefabricated needle arrays. No infection was obtained in repeated tests with barley yellow dwarf virus (BYDV-PAV) or cereal yellow dwarf virus (CYDV-RPV) using either sap or RNA from infectious cloned cDNA. Replacement of the original engraving-tool with a linear-motion tattoo machine in VPI provides greater flexibility and convenience in a quiet, readily-available instrument, while improving reproducibility through the use of prefabricated needle arrays.
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Abbreviations
- BYDV:
-
barley yellow dwarf virus
- CYDV:
-
cereal yellow dwarf virus
- MRFV:
-
maize rayado fino virus
- OBDV:
-
oat blue dwarf virus
- VPI:
-
vascular puncture inoculation
References
Agrios, G. N. (2005). Plant Pathology, 5th edition. Burlington: Elsevier Academic Press.
Clark, M. F., & Adams, A. N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol, 34, 475–483.
Edwards, M. C., & Weiland, J. J. (2009). Maize as a new host for oat blue dwarf virus. Plant Disease, 93, 972.
Edwards, M. C., & Weiland, J. J. (2010). First infectious clone of the propagatively transmitted Oat blue dwarf virus. Arch Virol, 155, 463–470.
Edwards, M. C., & Weiland, J. J. (2011). Presence of a polyA tail at the 3′ end of maize rayado fino virus RNA. Arch Virol, 156, 331–334.
Esau, K. (1957). Phloem degeneration in Gramineae affected by the barley yellow-dwarf virus. Am J Bot, 44, 245–251.
Gill, C. C., & Chong, J. (1975). Development of the infection in oat leaves inoculated with barley yellow dwarf virus. Virology, 66, 449–453.
Kidd, J. G. (1938). The course of virus-induced rabbit papillomas as determined by virus, cells, and host. J Exp Med, 67, 551–574.
Louie, R. (1995). Vascular puncture of maize kernels for the mechanical transmission of maize white line mosaic virus and other viruses of maize. Phytopathology, 85, 139–143.
Louie, R., Seifers, D. L., & Bradfute, O. E. (2006). Isolation, transmission and purification of the High Plains virus. J Virol Methods, 135, 214–222.
Madriz-Ordeñana, K., Montero, R. R., Lundsgaard, T., Ramírez, P., Thordal-Christensen, H., & Collinge, D. B. (2000). Mechanical transmission of maize rayado fino marafivirus (MRFV) to maize and barley by means of the vascular puncture technique. Plant Pathol, 49, 302–307.
Matthews REF (1991) Plant virology, 3rd Edition. (Academic Press)
Pokorná, D., Poláková, I., Kindlová, M., Dusková, M., Ludvíková, V., Gabriel, P., et al. (2009). Vaccination with human papillomavirus type 16-derived peptides using a tattoo device. Vaccine, 27, 3519–3529.
Quaak, S. G., van den Berg, J. H., Oosterhuis, K., Beijnen, J. H., Haanen, J. B., & Nuijen, B. (2009). DNA tattoo vaccination: effect on plasmid purity and transfection efficiency of different topoisoforms. J Control Release, 139, 153–159.
Redinbaugh, M. G. (2003). Transmission of Maize streak virus by vascular puncture inoculation with unit-length genomic DNA. J Virol Methods, 109, 95–98.
Redinbaugh, M. G., Louie, R., Ngwira, P., Edema, R., Gordon, D. T., & Bisaro, D. M. (2001). Transmission of viral RNA and DNA to maize kernels by vascular puncture inoculation. J Virol Methods, 98, 135–143.
Seifers, D. L., She, Y.-M., Harvey, T. L., Martin, T. J., Haber, S., Ens, W., et al. (2004). Biological and molecular variability among High Plains virus isolates. Plant Disease, 88, 824–829.
Weiland, J. J., & Edwards, M. C. (1994). Evidence that the alpha-a gene of barley stripe mosaic virus encodes determinants of pathogenicity to oat (Avena sativa). Virology, 201, 116–126.
Zeyen, R. J., & Banttari, E. E. (1972). Histology and ultrastructure of oat blue dwarf virus infected oats. Can J Bot, 50, 2511–2519.
Zhang, L., Zitter, T. A., & Lulkin, E. J. (1991). Artificial inoculation of maize white line mosaic virus into corn and wheat. Phytopathology, 81, 397–400.
Acknowledgments
The authors thank Renee McClean for technical assistance and Joe Anderson for the generous gift of BYDV/CYDV inoculum and reagents. We also thank Mike McMullen for providing ‘Paul’ oats and Steven Xu for triticale seed.
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Weiland, J.J., Edwards, M.C. Linear-motion tattoo machine and prefabricated needle sets for the delivery of plant viruses by vascular puncture inoculation. Eur J Plant Pathol 131, 553–558 (2011). https://doi.org/10.1007/s10658-011-9830-2
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DOI: https://doi.org/10.1007/s10658-011-9830-2