Abstract
Citrus post-bloom fruit drop (caused by Colletotrichum acutatum) frequently occurs in the southwestern region of São Paulo State, Brazil. A survey of Colletotrichum isolates associated with symptoms of post-bloom fruit drop in São Paulo State showed C. gloeosporioides in addition to C. acutatum. The objectives of this study were to confirm the identification of C. gloeosporioides isolated from symptomatic citrus flowers, to test the pathogenicity of C. gloeosporioides isolates, to compare the development of disease caused by C. gloeosporioides and C. acutatum, and to determine the frequency of C. gloeosporioides in a sample of isolates obtained from symptomatic flowers in different regions of São Paulo State. Through the use of species-specific primers by PCR, 17.3% of 139 isolates were C. gloeosporioides, and the remaining 82.7% were C. acutatum. The pathogenicity tests, carried out in 3-year old potted plants of sweet oranges indicated that both species caused typical symptoms of the disease including blossom blight and persistent calyces. Incubation periods (3.5 and 3.9 days, respectively, for C. acutatum and C. gloeosporioides) and fruit sets (6.7 and 8.5%, respectively for C. acutatum and C. gloeosporioides) were similar for both species. The incidences of blossom blight and persistent calyces were higher on plants inoculated with C. acutatum than in those inoculated with C. gloeosporioides. Conidial germination was similar for both species under different temperatures and wetness periods. Under optimal conditions, appressorium formation and melanisation were higher for C. gloeosporioides than for C. acutatum. These results indicated that Colletotrichum gloeosporioides is a new causal agent of post-bloom fruit drop.
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References
Bassanezi, R. B., Amorim, L., Bergamin Filho, A., & Hau, B. (1998). Effects of line pattern mosaic virus on the monocyclic components of rust and angular leaf spot of Phaseolus bean at different temperatures. Plant Pathology, 47, 289–298.
Brown, A. E., Sreenivasaprasad, S., & Timmer, L. W. (1996). Molecular characterization of slow-growing orange and Key lime anthracnose strains of Colletotrichum from citrus as C. acutatum. Phytopathology, 86, 523–527.
Christiano, R. S. C., Dalla Pria, M., Jesus Junior, W. C., Amorim, L., & Bergamin Filho, A. (2009). Modelling the progress of Asiatic citrus canker on Tahiti lime in relation to temperature and leaf wetness. European Journal of Plant Pathology, 124, 1–7.
De Goes, A., Garrido, R. B. O., Reis, R. F., Baldassari, R. B., & Soares, M. A. (2008). Evaluation of fungicide applications to sweet orange at different flowering stages for control of postbloom fruit drop caused by Colletotrichum acutatum. Crop Protection, 27, 71–76.
Fagan, H. J. (1979). Postbloom fruit drop, a new disease of citrus associated with a form of Colletotrichum gloeosporioides. Annals of Applied Biology, 91, 13–20.
Junghans, D. T., Gomes, E. A., Guimarães, W. V., Barros, E. G., & Araújo, E. F. (1998). Genetic diversity of the ectomycorrhizal fungus Pisolithus tinctorius based on RAPD-PCR analysis. Mycorrhiza, 7, 243–248.
Li, W., Yuan, R., Burns, J. K., Timmer, L. W., & Chung, K. R. (2003). Genes for hormone biosynthesis and regulation are highly expressed in citrus flowers infected with the fungus Colletotrichum acutatum, the causal agent of postbloom fruit drop. Journal of the American Society of Horticultural Science, 128, 578–583.
Mackenzie, S. J., Peres, N. A., Barquero, M. P., Arauz, L. F., & Timmer, L. W. (2009). Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida. Phytopathology, 99, 620–631.
Mills, P. R., Sreenivasaprasad, S., & Brown, A. E. (1992). Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR. FEMS Microbiology Letters, 98, 137–143.
Peres, N. A. R., Kuramae, E. E., Dias, M. S. C., & Souza, N. L. (2002a). Identification and characterization of Colletotrichum spp. affecting fruit after harvest in Brazil. Journal of Phytopathology, 150, 128–134.
Peres, N. A. R., Kim, S., Beck, H. W., Souza, N. L., & Timmer, L. W. (2002b). A fungicide application decision (FAD) support system postbloom fruit drop of citrus (PFD). On line. Plant Health Progress. doi:10.1094/PHP-2002-0731-01-RV.
Peres, N. A. R., Souza, N. L., Zitko, S. E., & Timmer, L. W. (2002c). Activity of benomyl for control of postbloom fruit drop of citrus caused by Colletotrichum acutatum. Plant Disease, 86, 620–624.
Peres, N. A., Timmer, L. W., Adaskaveg, J. E., & Correll, J. C. (2005). Lifestyles of Colletotrichum acutatum. Plant Disease, 89, 784–796.
Simmonds, J. H. (1965). A study of the species of Colletotrichum causing ripe fruit rots in Queensland. Queensland Journal of Agricultural and Animal Sciences, 22, 437–459.
Sreenivasaprasad, S., & Talhinhas, P. (2005). Genotypic and phenotypic diversity in Colletotrichum acutatum a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Molecular Plant Pathology, 6, 361–378.
Sreenivasaprasad, S., Sharada, K., Brown, A. E., & Mills, P. R. (1996). PCR-based detection of Colletotrichum acutatum on strawberry. Plant Pathology, 45, 650–655.
Sutton, B. C. (1992). The genus Glomerella and its anamorph Colletotrichum. In J. A. Bailey & M. J. Jeger (Eds.), Colletotrichum: Biology, Pathology, and Control (pp. 523–537). Wallingford: CAB International.
Talhinhas, P., Sreenivasaprasad, S., Neves-Martins, J., & Oliveira, H. (2002). Genetic and morphological characterization of Colletotrichum acutatum causing anthracnose of lupins. Phytopathology, 92, 986–996.
Talhinhas, P., Sreenivasaprasad, S., Neves-Martins, J., & Oliveira, H. (2005). Molecular and phenotypic analyses reveal association of diverse Colletotrichum acutatum groups and a low level of C. gloeosporioides with olive anthracnose. Applied and Environmental Microbiology, 71, 2987–2998.
Timmer, L. W., Agostini, J. P., Zitko, S. E., & Zulfiqar, M. (1994). Postbloom fruit drop, an increasingly prevalent disease of citrus in the America. Plant Disease, 78, 329–334.
Timmer, L. W., & Brown, G. E. (2000). Biology and control of anthracnose diseases of citrus. In D. Prusky, S. Freeman, & M. B. Dickman (Eds.), Colletotrichum. Host Specificity, Pathology, and Host-Pathogen Interaction (pp. 300–316). Saint Paul: APS.
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, & J. J. Sninsky (Eds.), PCR Protocols: A guide to methods and applications (pp. 315–322). San Diego: Academic.
Zar, J. H. (1999). Biostatistical analysis. Upper Saddle River: Prentice Hall
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We are grateful for the financial support from the agencies FAPESP (2008/54176-4) and CNPq (authors’ scholarships).
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Lima, W.G., Spósito, M.B., Amorim, L. et al. Colletotrichum gloeosporioides, a new causal agent of citrus post-bloom fruit drop. Eur J Plant Pathol 131, 157–165 (2011). https://doi.org/10.1007/s10658-011-9795-1
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DOI: https://doi.org/10.1007/s10658-011-9795-1