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Analysis of population structure of Puccinia striiformis in Yunnan Province of China by using AFLP

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Abstract

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most destructive wheat diseases in China. Yunnan Province, located in south-western China, possesses unique features of geography, climate, wheat growth and stripe rust epidemics, different from main epidemic regions in China. The isolates of this pathogen were collected from nine counties in Yunnan Province during February to May of 2008. Used as a comparison, isolates were also collected from five counties of Gansu Province, the province important in inter-regional stripe rust epidemics in China. Amplified fragment length polymorphism (AFLP) method was applied to study the population genetics of the pathogen among different populations in these two provinces. Forty one AFLP genotypes were obtained from 150 isolates and the genotype qj3 showed the highest frequency in Yunnan Province. While 22 genotypes were detected from 40 isolates, no genotype showing as predominant was identified in Gansu Province. Genotypic diversity in Gansu Province was higher than that in Yunnan Province. A free recombination signature was detected in Gansu Province but not in Yunnan Province. We concluded that the population of P. striiformis in Yunnan Province can be considered as a clonal population.

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Acknowledgements

The authors thank J.-Y. Zhou and J.-P. Liu, Yunnan Plant Protection Station (YPPS), for their valuable assistance in sample collection. The authors thank the following units under YPPS for their sampling efforts: Plant Protection Stations of Qiaojia, Eshan, Fuyuan, Yimen, Jiangchuan, Baoshan, Yuxi, Shidian and Yongren counties. The authors also thank S.-D. Shi, a GIS specialist at National Animal Husbandry Service & China Feed Industry Association, for his assistance in modification of the GIS map. This work was supported by “973 Project” (No. 2006CB100203) and by Special Public Sector Research Funding in China (No. 200903035 and No.200903004).

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Authors and Affiliations

  1. Department of Plant Pathology, China Agricultural University, Beijing, 100193, China

    Xiufeng Liu, Chong Huang, Zhenyu Sun, Junmin Liang, Yong Luo & Zhanhong Ma

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  1. Xiufeng Liu
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  2. Chong Huang
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  3. Zhenyu Sun
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  4. Junmin Liang
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  5. Yong Luo
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Correspondence to Yong Luo or Zhanhong Ma.

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Liu, X., Huang, C., Sun, Z. et al. Analysis of population structure of Puccinia striiformis in Yunnan Province of China by using AFLP. Eur J Plant Pathol 129, 43–55 (2011). https://doi.org/10.1007/s10658-010-9688-8

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