European Journal of Plant Pathology

, Volume 128, Issue 4, pp 483–493 | Cite as

Benzothiadiazole and BABA improve resistance to Uromyces pisi (Pers.) Wint. in Pisum sativum L. with an enhancement of enzymatic activities and total phenolic content

  • Eleonora BarilliEmail author
  • Elena Prats
  • Diego Rubiales


Benzothiadiazole (BTH) and DL-β-aminobutyric acid (BABA) induced systemic resistance was investigated in susceptible and resistant pea genotypes against Uromyces pisi. Resistance was characterized by reduced infection frequency mainly due to decreases in appressorium formation, stomatal penetration, growth of infection hyphae and haustorium formation. Changes in β-1,3-glucanase, chitinase, phenylalanine ammonia-lyase and peroxidase activities and in total phenolics content, demonstrate that U. pisi resistance is induced by BTH and BABA treatments at early and late stages of the fungal infection process, but that the chemicals operate via different mechanisms. In fact, our study showed that BTH treatment primed the activity of pathogenesis related-proteins such as β-1,3-glucanase, chitinase and peroxidase in both susceptible and resistant genotypes. On the other hand, BABA treatment did not increase the enzymatic activities in the studied genotypes, but significantly increased their total phenolic contents.


Chitinase Glucanase Pea rust Peroxidase Phenolics PR-protein 



DL-3-amino-n-butanoic (DL-β-aminobutyric) acid


Benzo [1,2,3]thiadiazole-7-carbothionic acid-S-methyl ester (Bion®)


Pathogenesis-related (proteins)


Thin layer chromatography



The authors are greatly indebted to the Spanish AGL2008-01239/AGR and Andalusian P07-AGR-02883 projects for financial support and to Ana Moral for technical assistance.


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© KNPV 2010

Authors and Affiliations

  1. 1.Institute for Sustainable AgricultureCSICCórdobaSpain

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