Abstract
In order to accelerate breeding and selection for disease resistance to Fusarium wilt, it is important to develop bioassays which can differentiate between resistant and susceptible cultivars efficiently. Currently, the most commonly used early bioassay for screening Musa genotypes against Fusarium oxysporum f. sp. cubense (Foc) is a pot system, followed by a hydroponic system. This paper investigated the utility of in vitro inoculation of rooted banana plantlets grown on modified medium as a reliable and rapid bioassay for resistance to Foc. Using a scale of 0 to 6 for disease severity measurement, the mean final disease severities of cultivars expressing different levels of disease reaction were significantly different (P ≤ 0.05). Twenty-four days after inoculation with Foc tropical race 4 at 106 conidia ml−1, the plantlets of two susceptible cultivars had higher final disease severities than that of four resistant cultivars. Compared with ‘Guangfen No.1’, ‘Brazil Xiangjiao’ is highly susceptible to tropical race 4 and its mean final disease severity was the highest (5.27). The plantlets of moderately resistant cultivar ‘Formosana’ had a mean final disease severity (3.53) lower than that of ‘Guangfen No.1’ (4.33) but higher than that of resistant cultivars: ‘Nongke No.1’, GCTCV-119, and ‘Dongguan Dajiao’ (1.87, 1.73, and1.53, respectively). Promising resistant clones acquired through non-conventional breeding techniques such as in vitro selection, genetic transformation, and protoplast fusion could be screened by the in vitro bioassay directly. Since there is no acclimatization stage for plantlets used in the bioassay, it helps to improve banana breeding efficiency.
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Abbreviations
- Foc :
-
Fusarium oxysporum f. sp. cubense
- MIS:
-
Medium for interaction system
- MS:
-
Murashige and Skoog medium
References
Arinaitwe, G., Rubaihayo, P. R., & Magambo, M. J. S. (1999). Effects of auxin/cytokinin combinations on shoot proliferation in banana cultivars. African Crop Science Journal, 7, 605–611.
Beckman, C. H., & Halmos, S. (1962). Relation of vascular occluding reactions in banana roots to pathogenicity of root-invading fungi. Phytopathology, 52, 893–897.
Bhagwat, B., & Duncan, E. J. (1998a). Mutation breeding of banana cv. Highgate for tolerance to Fusarium oxysporum f. sp. cubense using gamma irritation. Euphytica, 101, 143–150.
Bhagwat, B., & Duncan, E. J. (1998b). Mutation breeding of banana cv. Highgate (Musa spp. AAA Group) for tolerance to Fusarium oxysporum f. sp. cubense using chemical mutagens. Scientia Horticulturae, 73, 11–22.
Booth, C. (1977). Fusarium laboratory guide to the identification of the major species. Kew: Commonwealth Mycological Institute.
Buddenhagen, I. (2009). Understanding strain diversity in Fusarium oxysporum f. sp. cubense and history of introduction of ‘tropical race 4’ to better manage banana production. Acta Horticulturae, 828, 193–204.
Companioni, B., Arzola, M., Rodríguez, Y., Mosqueda, M., Pérez, M. C., Borrás, O., et al. (2003). Use of culture-derived Fusarium oxysporum f. sp. cubense, race 1 filtrates for rapid and non-destructive in vitro differentiation between resistant and susceptible clones of field-grown banana. Euphytica, 130, 341–347.
Couteaudier, Y., & Alabouvette, C. (1990). Survival and inoculum potential of conidia and chlamydospores of Fusarium oxysporum f. sp. lini in soil. Canadian Journal of Microbiology, 36, 551–556.
Daniells, J., Davis, D., Peterson, R., & Pegg, K. (1995). Goldfinger: not as resistant to sigatoka/yellow sigatoka as first thought. Infomusa, 4, 6.
De Ascensao, A. R. D. C. F., & Dubery, I. A. (2000). Panama disease: cell wall reinforcement in banana roots in response to elicitors from Fusarium oxysporum f. sp. cubense race four. Phytopathology, 90, 1173–1180.
Groenewald, S., Van den Berg, N., Marasas, W. F. O., & Viljoen, A. (2006). Biological, physiological and pathogenic variation in a genetically homogenous population of Fusarium oxysporum f.sp. cubense. Australasian Plant Pathology, 35, 401–409.
Huang, B. Z. (2005). Introduction of ‘Guangfen No.1’ (ABB). China Tropical Agriculture, (4), 38. (in Chinese)
Huang, B. Z., Xu, L. B., Yang, H., Tang, X. L., Wei, Y. R., Qiu, J. S., et al. (2005). Preliminary results of field evaluation of banana germplasm resistant to Fusarium wilt disease. Guangdong Agricultural Sciences, (6), 9–10. (in Chinese)
Hwang, S. C., & Ko, W. H. (2004). Cavendish banana cultivars resistant to Fusarium wilt acquired through somaclonal variation in Taiwan. Plant Disease, 88, 580–588.
Liu, S. Q., Liang, Z. H., Huang, Z. H., & Huang, Y. X. (2007). Selection of ‘Nongke No.1’ (Musa AAA Cavendish subgroup) resistant to Fusarium wilt. Guangdong Agricultural Sciences, (1), 30–32. (in Chinese)
Matsumoto, K., Barbosa, M. L., Souza, L. A. C., & Teixeira, J. B. (1995). Race 1 fusarium wilt tolerance on banana plants selected by fusaric acid. Euphytica, 84, 67–71.
Matsumoto, K., Barbosa, M. L., Souza, L. A. C., & Teixeira, J. B. (1999). In vitro selection for Fusarium wilt resistance in banana II. Resistance to culture filtrate of race 1 Fosarium oxysporum f. sp. cubense. Fruits, 54, 151–157.
Matsumoto, K., Vilarinhos, A. D., & Oka, S. (2002). Somatic hybridization by electrofusion of banana protoplast. Euphytica, 125, 317–324.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.
Ploetz, R. C. (2006). Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp. cubense. Phytopathology, 96, 653–656.
Smith, L. J., Smith, M. K., Tree, D., O’Keefe, D., & Galea, V. J. (2008). Development of a small-plant bioassay to assess banana grown from tissue culture for consistent infection by Fusarium oxysporum f. sp. cubense. Australasian Plant Pathology, 37, 171–179.
Subramaniam, S., Maziah, M., Sariah, M., Puad, M. P., & Xavier, R. (2006). Bioassay method for testing Fusarium wilt disease tolerance in transgenic banana. Scientia Horticulturae, 108, 378–389.
Tripathi, L., Odipio, J., Tripathi, J. N., & Tusiime, G. (2008). A rapid technique for screening banana cultivars for resistance to Xanthomonas wilt. European Journal of Plant Pathology, 121, 9–19.
Twizeyimana, M., Ojiambo, P. S., Tenkouano, A., Ikotun, T., & Bandyopadhyay, R. (2007). Rapid screening of Musa species for resistance to black leaf streak using in vitro plantlets in tubes and detached leaves. Plant Disease, 91, 308–314.
Vakili, N. G. (1965). Fusarium wilt resistance in seedlings and mature plants of Musa species. Phytopathology, 55, 135–140.
Van den Berg, N., Berger, D. K., Hein, I., Birch, P. R. J., Wingfield, M. J., & Viljoen, A. (2007). Tolerance in banana to Fusarium wilt is associated with early up-regulation of cell wall-strengthening genes in the roots. Molecular Plant Pathology, 8, 333–341.
Waite, B. H. (1963). Wilt of Heliconia spp. caused by Fusarium oxysporum f. sp. cubense race 3. Tropical Agriculture Trinidad, 40, 299–305.
Weber, O. B., Muniz, C. R., Vitor, A. O., Freire, F. C. O., & Oliveira, V. M. (2007). Interaction of endophytic diazotrophic bacteria and Fusarium oxysporum f. sp. cubense on plantlets of banana ‘Macã’. Plant and Soil, 298, 47–56.
Wong, W. C. (1986). In vitro propagation of banana (Musa spp.): initiation, proliferation and development of shoot-tip cultures on defined media. Plant Cell, Tissue and Organ Culture, 6, 159–166.
Wu, Y. L., Yi, G. J., Yang, H., Zhou, B. R., & Zeng, J. W. (2005). Basal medium with modified nitrogen source and other factors influence the rooting of banana. HortScience, 40, 428–430.
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Wu, Y.L., Yi, G.J. & Peng, X.X. Rapid screening of Musa species for resistance to Fusarium wilt in an in vitro bioassay. Eur J Plant Pathol 128, 409–415 (2010). https://doi.org/10.1007/s10658-010-9669-y
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DOI: https://doi.org/10.1007/s10658-010-9669-y