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Genetic variability and pathological properties of Grapevine Leafroll-associated Virus 2 isolates

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Abstract

The genetic variability among Grapevine leafroll-associated virus 2 (GLRaV-2) isolates was investigated in several grapevine accessions from various geographic origins in three genomic fragments, which encode the 70 kDa heat-shock protein homologue, the coat protein and the ∼60 kDa protein. The majority of the isolates were identical or only slightly different from one another and formed a monophyletic group. Several other variants were found which differed greatly from the main cluster and were mostly present in autochthonous or specific vine cultivars. Phylogenetic analyses on the CP gene sequences, including the isolates analyzed in this work and some others from related literature, allowed five clades to be identified (PN, H4, RG, BD and PV20). Biological trials for graft incompatibility and leafroll symptoms revealed that GLRaV-2 divergent variants had different pathological properties, mainly according to their phylogenetic grouping. The BD isolate seemed to be a mild variant of the virus, because it was unable to induce graft incompatibility and it rarely caused leafroll symptoms. The RG isolate appeared to be a more virulent variant, given the strong decrease in rooted grafted grapevines obtained in nursery, in particular with the use of certain rootstocks; however, it does not induce leafroll symptoms. The variants belonging to the PN group, which were the most widespread, caused both graft incompatibility and leafroll symptoms, according to the viral variant. Graft incompatibility also proved to depend strongly on the rootstock used.

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Abbreviations

BT:

Bootstrap

CP:

Coat protein

GLRaV-2:

Grapevine leafroll-associated virus 2

GRSPaV:

Grapevine rupestris stem pitting associated virus

HMA:

Heteroduplex Mobility Assay

HSP70:

Heat-shock protein 70

ML:

Maximum likelihood

MP:

Maximum parsimony

p60:

∼60 kDa protein

UPGMA:

Unweighted Pair Group Method with Arithmetic mean

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Acknowledgements

The authors are grateful to the following scientists who kindly supplied GLRaV-2-infected grapevine accessions: M. Fuchs (Cornell University, Geneva, NY, USA), P. Gugerli (RAC, Changins, Switzerland), D. Boscia, A. Minafra and P. Saldarelli (CNR, Bari, Italy). The authors are also very grateful to I. Bazzo, L. Filippin, L. Lovat, E. Patriarca, G. Perini (CRA-VIT, Conegliano, TV, Italy) and E. Trevisan (University of Venice, Italy) for their technical assistance and to R. Davison for checking the English text.

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Correspondence to Elisa Angelini.

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Supplementary Fig. 2

Leafroll symptoms on GLRaV-2 infected Cabernet franc indicator used in the biological trials (JPEG 176 kb)

Supplementary Fig. 3

Field biological trial for graft incompatibility: a) View of the nursery (JPEG 198 kb); b) Detail of a plantlet showing gratf incompatibility (JPEG 121 kb)

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Bertazzon, N., Borgo, M., Vanin, S. et al. Genetic variability and pathological properties of Grapevine Leafroll-associated Virus 2 isolates. Eur J Plant Pathol 127, 185–197 (2010). https://doi.org/10.1007/s10658-010-9583-3

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