Abstract
Twenty-three isolates of Colletotrichum gloeosporioides, five isolates of C. acutatum, two isolates of C. capsici and six isolates of C. boninense associated with anthracnose disease on coffee (Coffea spp.) in Vietnam were identified based on morphology and DNA analysis. Phylogenetic analysis of DNA sequences from the internal transcribed spacer region of nuclear rDNA and a portion of mitochondrial small subunit rRNA were concordant and allowed good separation of the taxa. We found several Colletotrichum isolates of unknown species and their taxonomic position remains unresolved. The majority of Vietnamese isolates belonged to C. gloeosporioides and they grouped together with the coffee berry disease (CBD) fungus, C. kahawae. However, C. kahawae could be distinguished from the Vietnamese C. gloeosporioides isolates based on ammonium tartrate utilization, growth rate and pathogenicity. C. gloeosporioides isolates were more pathogenic on detached green berries than isolates of the other species, i.e. C. acutatum, C capsici and C. boninense. Some of the C. gloeosporioides isolates produced slightly sunken lesions on green berries resembling CBD symptoms but it did not destroy the bean. We did not find any evidence of the presence of C. kahawae in Vietnam.
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Acknowledgements
The authors acknowledge the Swedish International Development Agency (Sida) for financial support. We are thankful to Dr Sreenivasaprasad for kindly supplying isolates NI 90, 397 and PR220 used in this study. We also thank Ms Therese Bengtsson, Hoang Thi Ngat and Ann-Charlotte Strömdahl for excellent technical assistances. We acknowledge Mrs. Tran Kim Loang, Bui Thi Tram, Nguyen Thanh Ha, Mr Nguyen Cong Huyen, Le Quang Tu and coworkers in Dac Lak and Lam Dong province for valuable field trip arrangements and assistance during field samplings.
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Nguyen, P.T.H., Vinnere Pettersson, O., Olsson, P. et al. Identification of Colletotrichum species associated with anthracnose disease of coffee in Vietnam. Eur J Plant Pathol 127, 73–87 (2010). https://doi.org/10.1007/s10658-009-9573-5
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DOI: https://doi.org/10.1007/s10658-009-9573-5