Skip to main content
SpringerLink
Log in

Detection and characterization of Citrus tristeza virus stem pitting isolates in Jamaica

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

An island wide survey for Citrus tristeza virus (CTV) in citrus orchards across Jamaica (13 regions) was conducted over 2 years. Trees (1, 885) showing virus-like symptoms as well as asymptomatic trees were randomly sampled for testing by ELISA and 55 samples from the 6 major citrus growing regions were graft inoculated on indicator plants. Most samples (74%) reacted to polyclonal antibodies against CTV in ELISA, while 20% were positive in tests using monoclonal antibodies specific to severe CTV strains. Samples collected from the 6 major citrus growing regions produced vein clearing and stem pitting symptoms on Mexican lime indicator plants (87%). In addition, stem pitting symptoms were induced on Duncan grapefruit, sweet orange, sour orange or sweet orange grafted on sour orange. Nucleotide sequencing of the coat protein gene sequences isolated from these samples indicated high identities (88 to 95.5%) among the Jamaican isolates and previously reported stem pitting strains from Central and North America and Eurasia (88 to 100%). The results suggest a shared ancestry with isolates from other geographical locations, rather than geographical speciation, and presumably separate CTV introductions into Jamaica.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Barzegar, A., Sohi, H. H., & Rahimian, H. (2006). Characterization of Citrus tristeza virus isolates in northern Iran. Journal of General Plant Pathology, 72, 46–51.

    Article  CAS  Google Scholar 

  • Corazza-Nunes, M. J., Machado, M. A., Stach-Machado, D. R., Nunes, W. M. C., Alves de Carvalho, S., & Müller, G. W. (2006). Characterization of Citrus tristeza virus isolates from grapefruit (Citrus paradisi Macf.) accessions of citrus active germplasm bank. Summa Phytopathol, 32, 322–327.

    Article  Google Scholar 

  • Edman, F., & Young, F. (1999). Citrus tristeza virus survey 1999. Ministry of Agriculture, Bodles Agric. Research Station.

  • Febres, V. J., Niblett, C. L., Lee, R. F., & Moore, G. A. (2003). Characterization f grapefruit plants (Citrus paradise Macf.) transformed with citrus tristeza closterovirus genes. Plant Cell reports, 21, 421–428.

    CAS  PubMed  Google Scholar 

  • Garnsey, S. M., Gumpf, D. J., Roistacher, C. N., Civerolo, E., Lee, R. F., Yokomi, R. K., et al. (1987). Toward a standard evaluation of the biologically properties of Citrus tristeza virus. Phytophylactica, 19, 151–157.

    Google Scholar 

  • Garnsey, S. M. & Cambra, M. (1991). Enzyme-linked immunosorbent assay (ELISA) for citrus pathogens. In C. N. Roistacher (Ed.), Graft ransmissible diseases of citrus. Handbook for detection and diagnosis (pp. 193–216). Rome: Food and Agriculture Organization.

    Google Scholar 

  • Halbert, S. E., Genc, H., Cevik, B., Brown, L. G., Rosales, I. M., Manjunath, K. L., et al. (2004). Distribution and characterization of Citrus tristeza virus in south Florida following the establishment of Toxoptera citricida. Plant Disease, 88, 935–941.

    Article  Google Scholar 

  • Huang, Z., Rundell, P., Guan, X., & Powell, C. A. (2004). Detection and isolate differentiation of Citrus tristeza virus in infected field trees based on reverse transcription-polymerase chain reaction. Plant Disease, 88, 625–629.

    Article  CAS  Google Scholar 

  • Iglesias, N. G., Gago-Zachert, S. P., Robledo, G., Costa, N., Plata, M. I., Vera, O., et al. (2008). Population structure of Citrus tristeza virus from field Argentinean isolates. Virus Genes, 36, 199–207.

    Article  CAS  PubMed  Google Scholar 

  • Lee, R., McConnell, P., Majunath, K., Cevik, B., Nikolaeva, O., Dekkers, M., et al. (2002). The Citrus tristeza virus epidemic in Bog Walk Valley, Jamaica. Paper presented at the 15th Conference of the International Organization of Citrus Virologists, Riverside, CA, USA.

  • Lee, R. F. & Bar-Joseph, M. (2000). Tristeza. In L. W. Timmer, S. M. Garnsney & J. H. Graham (Eds.), Compendium of citrus diseases (pp. 61–63). St. Paul: American Phytopathological Society Press.

    Google Scholar 

  • Lee, R. F., Baker, P. S., & Rocha-Peña, M. A. (1994). The Citrus tristeza virus (CTV): An introduction to current priorities, with special reference to the worsening situation in Central America and the Caribbean. International Institute of Biological Control (CAB International), Silwood Park, Ascot, Berks. UK.

  • Martín, S., Sambade, A., Rubio, L., Vives, M. C., Moya, P., Guerri, J., et al. (2009). Contribution of recombination and selection to molecular evolution of Citrus tristeza virus. Journal of General Virology, 90, 1527–1538.

    Article  PubMed  Google Scholar 

  • Moreno, P., Ambrós, S., Albiach-Marti, M. R., Guerri, J., & Peña, L. (2008). Citrus tristeza virus: a pathogen that changed the course of the citrus industry. Molecular Plant Pathology, 9, 251–268.

    Article  CAS  PubMed  Google Scholar 

  • Müller, G. W., Guirado, N., Figueiredo, J. O., Machado, M. A., Laranjeira, F. F., & Castro, J. L. (1996). Citrus tristeza virus causes stem pitting in Rangpur Lime rootstock grafted with some Mandarin cultivars in Capão Bonito, Brazil. In: Conference of the International Organization of Citrus Virologists IOCV, Riverside California 13, 325–328.

  • Myers, L., & Amin, H. A. (2004). New approaches for diagnosis and prevention of tristeza outbreaks. CTV detection and strain typing by PCR based methods. March 1–12, 2004. Final Report. Project FIGCF/03.

  • Papayiannis, L. C., Santos, C., Kyriakou, A., Kapari, T., & Nolasco, G. (2007). Molecular characterization of Citrus tristeza virus isolates from Cyprus on the basis of the coat protein gene. Journal of Plant Pathology, 89, 291–295.

    CAS  Google Scholar 

  • Penaranda, J., Acosta, O., Guzman-Barney, M., Alegria, A., Pappu, H. R., Pappu, S. S., Manjunath, K. L., Febres, V. J., Lee, R. F., & Niblett, C. L. (1996). Incidence and characterization of mild and severe isolates of citrus tristeza virus from Colombia. In: Proc 13th Conf Inter Org Citrus Virol, Riverside, CA, pp 71–77.

  • Permar, T. A., Garnsey, S. M., Gumpf, D. F., & Lee, R. F. (1990). A monoclonal antibody that discriminates strains of Citrus tristeza virus. Phytopathology, 80, 224–228.

    Article  Google Scholar 

  • Rocha-Peña, M. A., Lee, R. F., Lastra, R., Niblett, C. L., Ochoa-Corona, F. M., Garnsey, S. M., et al. (1995). Citrus tristeza virus and its aphid vector Toxoptera citricida: Threats to citrus production in the Caribbean and Central North America. Plant Disease, 79, 437–444.

    Google Scholar 

  • Roistacher, C. N. (1991). Graft transmissible diseases of citrus: Handbook for detection and diagnosis. Rome: Food and Agriculture Organization.

    Google Scholar 

  • Rubio, L., Ayllón, M. A., Kong, P., Fernández, A., Polek, M. L., Guerri, J., et al. (2001). Genetic variation of Citrus tristeza virus isolates from California and Spain: evidence for mixed infections and recombination. Journal of Virology, 75, 8054–8062.

    Article  CAS  PubMed  Google Scholar 

  • Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighing, position specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.

    Article  CAS  PubMed  Google Scholar 

  • Turturo, C., Saldarelli, P., Yafeng, D., Digiaro, M., Minafra, A., Savino, V., et al. (2005). Genetic variability and population structure of Grapevine leafroll-associated virus 3 isolates. Journal of General Virology, 86, 217–224.

    Article  CAS  PubMed  Google Scholar 

  • Young, A. (2002). Ministry’s Views. Citrus Replanting News, Ministry of Agriculture Jamaica. 1(4).

Download references

Acknowledgements

This research was funded by the Jamaican Ministry of Agriculture and the New Initiative Fund (UWI, Mona). We thank members of staff of the Bodles Research Station, Citrus Trade Winds Limited, Biotechnology Centre and University of Florida for technical assistance, assistance in conducting the survey and provision CTV sequences.

Author information

Authors and Affiliations

  1. Department of Life Sciences, The University of the West Indies, Kingston 7, Mona Campus, Kingston, Jamaica

    Latanya C. Fisher & Paula F. Tennant

  2. The Biotechnology Centre, The University of the West Indies, Kingston 7, Mona Campus, Kingston, Jamaica

    Paula F. Tennant

  3. Department of Basic Medical Sciences (Biochemistry Section), The University of the West Indies, Kingston 7, Mona Campus, Kingston, Jamaica

    Wayne A. McLaughlin

Authors
  1. Latanya C. Fisher
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paula F. Tennant
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wayne A. McLaughlin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Paula F. Tennant.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fisher, L.C., Tennant, P.F. & McLaughlin, W.A. Detection and characterization of Citrus tristeza virus stem pitting isolates in Jamaica. Eur J Plant Pathol 127, 1–6 (2010). https://doi.org/10.1007/s10658-009-9572-6

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-009-9572-6

Keywords

Search

Navigation