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Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic

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Abstract

In this paper, we report a large-scale survey for the incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) in stone fruit collections and commercial orchards in the Czech Republic. From the 645 samples analysed, PLMVd was detected in 80 (26.6%) of peaches and the HSVd in 3 (1.3%) of apricot and 1 (0.33%) of peach trees. Sixty-seven accession of peach (44.6%) from the Czech Clonal GeneBank were infected by PLMVd. In addition, we used naturally infected trees to standardise the simultaneous detection of PLMVd and HSVd plus host mRNA as the control by means of one-step multiplex RTC-PCR. Eleven PLMVd and two HSVd isolates were sequenced and analysed. All the PLMVd variants were highly homologous (97–100%) to previously reported PLMVd variants from Tunisian peach and almond trees, and clustered together in the previously reported phylogenetic group III. The HSVd variants obtained from apricot and peach trees were included in the previously proposed recombinant group PH/cit3.

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Acknowledgements

We thank Mr. P. Thomas for checking the English grammar. This work was supported by grants BIO2005-07331 and BIO-2008-03528 from the Spanish Ministry of Science and Innovation. G. Gomez is the recipient of a contract from the CSIC.

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Authors and Affiliations

  1. Plant Pathology Division, Faculty of Agriculture, Fayoum University, Fayoum, Egypt

    M. Hassan

  2. Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture, Prague, Czech Republic

    M. Hassan & P. Rysanek

  3. Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Avenida de los Naranjos s/n 46022, Valencia, Spain

    G. Gomez & V. Pallás

  4. Certis Europe B.V., Via A. Guaragna, 3-21047, Saronno, VA, Italy

    A. Myrta

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  1. M. Hassan
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  2. G. Gomez
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  3. V. Pallás
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  4. A. Myrta
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  5. P. Rysanek
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Correspondence to M. Hassan.

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Hassan, M., Gomez, G., Pallás, V. et al. Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic. Eur J Plant Pathol 124, 363–368 (2009). https://doi.org/10.1007/s10658-008-9420-0

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