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Trichoderma harzianum elicits defence response genes in roots of potato plantlets challenged by Rhizoctonia solani

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Abstract

Biological control of Rhizoctonia solani with Trichoderma harzianum has been demonstrated in several studies. However, none have reported the dynamics of expression of defence response genes. Here we investigated the expression of these genes in potato roots challenged by R. solani in the presence/absence of T. harzianum Rifai MUCL 29707. Analysis of gene expression revealed an induction of PR1 at 168 h post-inoculation (hpi) and PAL at 96 hpi in the plants inoculated with T. harzianum Rifai MUCL 29707, an induction of PR1, PR2 and PAL at 48 hpi in the plants inoculated with R. solani and an induction of Lox at 24 hpi and PR1, PR2, PAL and GST1 at 72 hpi in the plants inoculated with both organisms. These results suggest that in the presence of T. harzianum Rifai MUCL 29707, the expression of Lox and GST1 genes are primed in potato plantlets infected with R. solani at an early stage of infection.

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Abbreviations

BCA:

biological control agent

β-tub:

beta-tubulin

EF1-α:

elongation factor 1-alpha

GAPDH:

glyceraldehyde phosphate dehydrogenase

GST1:

Gluthatione-S-transferase 1

Lox:

Lipoxygenase

PAL:

Phenylalanine ammonia lyase

PR1:

Pathogenesis Related 1

PR2:

Pathogenesis Related 2

R-treatment:

Rhizoctonia solani treatment

T-treatment:

Trichoderma harzianum treatment

T+R-treatment:

Trichoderma harzianum and Rhizoctonia solani treatment

Ubc:

ubiquitin conjugating enzyme-like

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Acknowledgements

This work was supported by the Direction Générale de l’Agriculture of the Walloon Region under contract number D31-1149, entitled « valorisation de la microflore bénéfique des sols pour le contrôle de la flore pathogène des productions de pomme de terre comme alternative à l’utilisation des pesticides». S.C. gratefully acknowledges the financial support from the Belgian Federal Science Policy Office (contract BCCM C3/10/003).

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Authors and Affiliations

  1. Unité de microbiologie, Université catholique de Louvain (UCL), Place croix du Sud 3, 1348, Louvain-la-Neuve, Belgium

    Adrien Gallou & Stéphane Declerck

  2. Unité de Microbiologie—Mycothèque de l’Université catholique de Louvain (MUCL), Université catholique de Louvain (UCL), Place croix du Sud 3, 1348, Louvain-la-Neuve, Belgium

    Sylvie Cranenbrouck

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  1. Adrien Gallou
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  2. Sylvie Cranenbrouck
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Correspondence to Stéphane Declerck.

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Mycothèque de l’Université catholique de Louvain of S. Cranenbrouck's affiliation is part of the Belgian Coordinated Collections of Micro-organisms (BCCM).

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Gallou, A., Cranenbrouck, S. & Declerck, S. Trichoderma harzianum elicits defence response genes in roots of potato plantlets challenged by Rhizoctonia solani . Eur J Plant Pathol 124, 219–230 (2009). https://doi.org/10.1007/s10658-008-9407-x

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