Abstract
New Xanthomonas isolates causing citrus bacterial canker in Korea were differentiated primarily on the basis of host range by comparison with reference strains. The new isolates were pathogenic to Citrus sinensis, C. paradisi, C. limon and C. unshiu and formed crater-like canker on the plants; this indicated that they were X. axonopodis (X.a.) pv. citri A types. Molecular characterization using rep-PCR fingerprinting and 16S rDNA sequence analysis was also performed. Cluster analysis by combining the band patterns of ERIC-, BOX- and REP-PCR clearly separated one group including only X. a. pv. citrumelo and the other group including X. a. pv. citri and X. a. pv. aurantifolii strains. There was a clear separation between X. a. pv. citri Asiatic types and X. a. pv. aurantifolii B, C types in the second group. Partial sequence analysis of 16S rDNA revealed that all strains of X. a. pv. aurantifolii B and C type, and X. a. pv. citrumelo formed a distinct cluster with a similarity of 99%. Our results indicate that the isolates causing citrus canker in Korea belong to the A type of X. a. pv. citri.
This is a preview of subscription content, access via your institution.
References
Brunings, A. M., & Gabriel, D. W. (2003). Xanthomonas citri: Breaking the surface. Molecular Plant Pathology, 4, 141–157.
Cubero, J., & Graham, J. H. (2002). Genetic relationship among worldwide strains of Xanthomonas causing canker in citrus species and design of new primers for their identification by PCR. Applied Environmental Microbiology, 68, 1257–1264.
Cubero, J., & Graham, J. H. (2004). The leucine-responsive regulatory protein (lrp) gene for characterization of the relationship among Xanthomonas species. International Journal of Systematic and Evolutionary Microbioogy, 54, 429–437.
Fox, G. E., Wisotzkey, J. D., & Jurtshuk Jr., P. (1992). How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. International Journal of Systematic and Evolutionary Microbioogy, 42, 166–170.
Lane, D. J. (1991). 16S/23S rRNA sequencing. In E. Stackebrandt, & M. Goodfellow (Eds.) Bacterial Systematics (pp. 115–175). New York: Wiley.
Louws, F. J., Fulbright, D. W., Stephens, C. T., & de Bruijn, F. J. (1994). Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Applied Environmental Microbiology, 60, 2286–2295.
Louws, F. J., Fulbright, D. W., Stephens, C. T., & de Bruijn, F. J. (1995). Differentiation of genomic structure by rep-PCR fingerprinting to rapidly classify Xanthomonas campestris pv. vesicatoria. Phytopathology, 85, 528–536.
Louws, F. J., Rademaker, J. L. W., & de Bruijn, F. J. (1999). The three Ds of PCR-based genomic analysis of phytobacteria: Diversity, detection, and disease diagnosis. Annual Review of Phytopathology, 37, 81–125.
Opgenorth, D. C., Smart, C. D., Louws, F. J., de Bruijn, F. J., & Kirkpatrick, B. C. (1996). Identification of Xanthomonas fragariae field isolates by rep-PCR genomic fingerprintings. Plant Disease, 80, 868–873.
Rohlf, F. J. (2000). NTSYSpc: Numerical taxonomy system, ver. 2.1. New York: Exeter.
Sambrook, J., Fritsch, E. F., & Maniatis, T. (Eds.). (1989). Molecular cloning: A laboratory manual (2nd ed). New York: Cold Spring Harbor Laboratory.
Shiotani, H., Ozaki, K., & Tsuyumu, S. (2000). Pathogenic interactions between Xanthomonas axonopodis pv. citri and cultivars of pummelo (Citrus grandis). Phytopathology, 90, 1383–1389.
Stall, R. E., & Civerolo, E. L. (1993). Xanthomonas campestris pv. citri: Cause of citrus canker. In J. G. Swings, & E. L. Civerolo (Eds.) Xanthomonas (pp. 48–51). London: Chapman and Hall.
Trindade, L. C., Lima, M. F., & Ferreira, M. A. S. V. (2005). Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting. Fitopatologia Brasileira, 30, 46–54.
Verniere, C., Hartung, J. S., Pruvost, O. P., Civerolo, E. L., Alvarez, A. M., Maestri, P., et al. (1998). Characterization of phenotypically distinct strains of Xanthomonas axonopodis pv. citri from Southwest Asia. European Journal of Plant Pathology, 104, 477–487.
Acknowledgement
This research was supported by Agriculture R & D Promotion Centre (ARPC), Ministry of Agriculture and Forestry, Korea, 2005 project (105058-03-2-HD120).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, Y.H., Lee, S., Lee, D.H. et al. Differentiation of citrus bacterial canker strains in Korea by host range, rep-PCR fingerprinting and 16S rDNA analysis. Eur J Plant Pathol 121, 97–102 (2008). https://doi.org/10.1007/s10658-007-9242-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10658-007-9242-5