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Evaluation of nematicidal properties of saponins from Medicago spp.

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Abstract

The nematicidal activity of saponins from Medicago arborea (tops), M. arabica (tops and roots) and M. sativa (tops and roots) against the plant-parasitic nematode Xiphinema index was investigated. Nematicidal activity of related prosapogenins and sapogenins on X. index is also described. Saponins from Medicago spp. at different concentrations were all nematicidal, those from M. arborea tops being the less effective. In general, saponins induced 100% mortality at 500 μg ml−1 between 8 and 48 h, while prosapogenins resulted in toxicity starting at 125 μg ml−1. Differences in the effects on X. index induced by prosapogenins and sapogenins were less pronounced, although prosapogenins displayed a larger range of activity. Assays with purified sapogenins demonstrated the relationship of the observed nematicidal activity of M. sativa and M. arborea to the content of the main aglycones (medicagenic acid and hederagenin, respectively) in the saponin extracts. Hederagenin displayed the highest bioactivity, giving 38% mortality after 1 h at 125 μg ml−1.

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Acknowledgement

The authors are grateful to the Italian “Ministero dell’Università e della Ricerca Scientifica” and “Ministero delle Politiche Agricole e Forestali” for financial support.

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Correspondence to Pinarosa Avato.

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Argentieri, M.P., D’Addabbo, T., Tava, A. et al. Evaluation of nematicidal properties of saponins from Medicago spp.. Eur J Plant Pathol 120, 189–197 (2008). https://doi.org/10.1007/s10658-007-9207-8

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  • DOI: https://doi.org/10.1007/s10658-007-9207-8

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