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AFLP analysis of Russian Alternaria tenuissima populations from wheat kernels and other hosts

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Abstract

Alternaria tenuissima is a common pathogen on a number of plants described in several geographic regions of the world. Genetic variation within and between Russian Far East, North West and Caucasus populations of A. tenuissima from wheat was examined. In addition, genetic differences between isolates from various hosts were estimated. In total, 101 isolates of A. tenuissima were studied using amplified fragment length polymorphism (AFLP) with four primer combinations. Wright’s fixation index (F st), gene flow (N m) and gene diversity (H s) were calculated. AFLP banding patterns indicated significant genetic distance and at the same time a low level of gene flow between the Far East and the two other groups of isolates originating from the European part of country. The degree of similarity between the North West and Caucasus populations was very high, as was the migration rate. Isolates analysed by UPGMA-based cluster analysis were grouped according to location of origin but irrespective of plant host. Based on the F st value, the group of isolates originating from wheat and barley were not found to differ significantly from each other.

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Acknowledgements

This work was supported in part by The Research Council of Norway, Fellowship programme for collaboration within higher education and research between Norway and Northwestern Russia (EJ/hsm IS Rus 03/04-89). The authors are grateful to Dr. E. G. Simmons (USA) for providing cultures.

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Authors and Affiliations

  1. All-Russian Institute of Plant Protection, Podbelskogo shosse 3, St. Petersburg, Pushkin, 196608, Russia

    Philipp B. Gannibal & Mark M. Levitin

  2. Plant Health and Plant Protection Division, Bioforsk – Norwegian Institute of Agricultural and Environmental Research, Høgskoleveien 7, Aas, 1432, Norway

    Sonja S. Klemsdal

Authors
  1. Philipp B. Gannibal
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  2. Sonja S. Klemsdal
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  3. Mark M. Levitin
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Correspondence to Philipp B. Gannibal.

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Gannibal, P.B., Klemsdal, S.S. & Levitin, M.M. AFLP analysis of Russian Alternaria tenuissima populations from wheat kernels and other hosts. Eur J Plant Pathol 119, 175–182 (2007). https://doi.org/10.1007/s10658-007-9159-z

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  • DOI: https://doi.org/10.1007/s10658-007-9159-z

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