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Effects of plant growth-promoting rhizobacteria on nodulation of Phaseolus vulgaris L. are dependent on plant P nutrition

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Abstract

Several plant growth-promoting rhizobacteria (PGPR) have shown potential to enhance nodulation of legumes when coinoculated with Rhizobium. To optimize the efficiency of these Rhizobium-PGPR-host plant interactions, unravelling the underlying mechanisms and analyzing the influence of specific environmental conditions is crucial. In this work the effect of four PGPR strains on the symbiotic interaction between Rhizobium and common bean (Phaseolus vulgaris) was studied under deficient versus sufficient phosphorus supply. It was observed that the effect on nodulation of three out of four PGPR tested was strongly dependent on P nutrition. Further, the use of specific PGPR mutant strains indicated that bacterial indole-3-acetic-acid production (IAA) and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity play an important role in the host nodulation response, particularly under low P conditions. Moreover, it was shown that the differential response to PGPR under low versus high P conditions was associated with changes in the host hormone sensitivity for nodulation induced under P deficiency. These findings contribute to the understanding of the interplay between Rhizobium, PGPR and the plant host under different environmental settings.

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Acknowledgements

R.R. is a recipient of a predoctoral fellowship from the ‘Vlaamse Interuniversitaire Raad (VLIR)’ R.T. acknowledges the receipt of a predoctoral fellowship from the Katholieke Universiteit Leuven. We thank Dr. Bernard Glick for kindly providing us the strain P. putida UW4 and strain UW4/AcdS−.

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  1. Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics, K.U. Leuven, Kasteelpark Arenberg 20, 3001, Heverlee, Belgium

    Roseline Remans, Anja Croonenborghs, Roldan Torres Gutierrez, Jan Michiels & Jos Vanderleyden

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  1. Roseline Remans
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  2. Anja Croonenborghs
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Correspondence to Roseline Remans.

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Remans, R., Croonenborghs, A., Gutierrez, R.T. et al. Effects of plant growth-promoting rhizobacteria on nodulation of Phaseolus vulgaris L. are dependent on plant P nutrition. Eur J Plant Pathol 119, 341–351 (2007). https://doi.org/10.1007/s10658-007-9154-4

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