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Tomato leaf curl Guangxi virus is a distinct monopartite begomovirus species

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Abstract

Three begomovirus isolates were obtained from tomato plants showing leaf curl symptoms in Guangxi province of China. Typical begomovirus DNA components representing the three isolates (GX-1, GX-2 and GX-3) were cloned and their full-length sequences were determined to be 2752 nucleotides. Nucleotide identities among the three viral sequences were 98.9–99.7%, but all shared <86.7% nucleotide sequence identity with other reported begomoviruses. The sequence data indicated that GX-1, GX-2 and GX-3 are isolates of a distinct begomovirus species for which the name Tomato leaf curl Guangxi virus (ToLCGXV) is proposed. Further analysis indicated that ToLCGXV probably originated through recombination among viruses related to Ageratum yellow vein virus, Tomato leaf curl China virus and Euphorbia leaf curl virus. PCR and Southern blot analyses demonstrated that isolates GX-1 and GX-2 were associated with DNAβ components, but not isolate GX-3. Sequence comparisons revealed that GX-1 and GX-2 DNAβ components shared the highest sequence identity (86.2%) with that of Tomato yellow leaf curl China virus (TYLCCNV). An infectious construct of ToLCGXV isolate GX-1 (ToLCGXV-GX) was produced and determined to be highly infectious in Nicotiana benthamiana, N. glutinosa, tobacco cvs. Samsun and Xanthi, tomato and Petunia hybrida plants inducing leaf curl and stunting symptoms. Co-inoculation of tomato plants with ToLCGXV-GX and TYLCCNV DNAβ resulted in disease symptoms similar to that caused by ToLCGXV-GX alone or that observed in infected field tomato plants.

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Acknowledgements

We thank Dr. Xinshun Ding (The Samuel Roberts Noble Foundation, OK, USA) for critical review of the manuscript. This work was supported by the National Natural Science Foundation of China (Grant No. 30530520) and the National Key Basic Research and Development Programme (2006CB101903).

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Authors and Affiliations

  1. State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, P.R. China

    Youping Xu, Xinzhong Cai & Xueping Zhou

  2. Center of Analysis and Measurement, Zhejiang University, Hangzhou, 310029, P.R. China

    Youping Xu

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  1. Youping Xu
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  2. Xinzhong Cai
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Correspondence to Xueping Zhou.

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Xu, Y., Cai, X. & Zhou, X. Tomato leaf curl Guangxi virus is a distinct monopartite begomovirus species. Eur J Plant Pathol 118, 287–294 (2007). https://doi.org/10.1007/s10658-007-9143-7

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