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Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross-protection

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Abstract

Papaya ringspot virus (PRSV) HA 5-1, a nitrous acid-induced mild mutant of severe strain HA, widely applied for control of PRSV by cross-protection, was used to study the genetic basis of attenuation. Using infectious clones, a series of recombinants was generated between HA 5-1 and HA and their infectivity was analyzed on the systemic host papaya and the local lesion host Chenopodium quinoa. The recombinants that contained mutations in P1 and HC-Pro genes caused attenuated infection on papaya without conspicuous symptoms, similar to HA 5-1. The recombination and sequence analyses strongly implicated two amino acid changes in the C-terminal region of P1 and two in HC-Pro of HA 5-1 involved in the attenuated infection on papaya. The recombinants that infected C. quinoa plants without local lesions contained the same mutations in the C-terminal region of HC-Pro for attenuated infection on papaya. We conclude that both P1 and HC-Pro bear important pathogenicity determinants for the infection on the systemic host papaya and that the mutations in HC-Pro affecting pathogenicity on papaya are also responsible for the inability to induce hypersensitive reaction on C. quinoa.

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Acknowledgements

The authors thank Drs. M. J. Chen, S. T. Hsu, and H. T. Hsu for their encouragement and advice. This research was partly supported by the grants NSC 87-2312-B-005-005 and NSC 89-2321-B-005-001 from the National Science Council of the Republic of China on Taiwan.

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Authors and Affiliations

  1. Department of Molecular Biotechnology, Da Yeh University, Chang Hwa, Taiwan

    Chu-Hui Chiang

  2. Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan

    Chun-Yee Lee, Ching-Hsien Wang, Fuh-Jyh Jan, Shih-Shun Lin, Tsung-Chi Chen, Joseph A. J. Raja & Shyi-Dong Yeh

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  1. Chu-Hui Chiang
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  2. Chun-Yee Lee
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Correspondence to Shyi-Dong Yeh.

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Chiang, CH., Lee, CY., Wang, CH. et al. Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross-protection. Eur J Plant Pathol 118, 333–348 (2007). https://doi.org/10.1007/s10658-007-9130-z

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