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Validation of a QTL for resistance to ascochyta blight linked to resistance to fusarium wilt race 5 in chickpea (Cicer arietinum L.)

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Abstract

Ascochyta blight caused by Ascochyta rabiei and fusarium wilt caused by Fusarium oxysporum. f. sp. ciceris are the two most serious diseases of chickpea (Cicer arietinum). Quantitative trait loci (QTL) or genes for ascochyta blight resistance and a cluster of resistance genes for several fusarium wilt races (foc1, foc3, foc4 and foc5) located on LG2 of the chickpea map have been reported independently. In order to validate these results and study the linkage relationship between the loci that confer resistance to blight and wilt, an intraspecific chickpea recombinant inbred lines (RIL) population that segregates for resistance to both diseases was studied. A new LG2 was established using sequence tagged microsatellite sites (STMS) markers selected from other chickpea maps. Resistance to race 5 of F. oxysporum (foc5) was inherited as a single gene and mapped to LG2, flanked by the STMS markers TA110 (6.5 cM apart) and TA59 (8.9 cM apart). A QTL for resistance to ascochyta blight (QTLAR3) was also detected on LG2 using evaluation data obtained separately in two cropping seasons. This genomic region, where QTLAR3 is located, was highly saturated with STMS markers. STMS TA194 appeared tightly linked to QTLAR3 and was flanked by the STMS markers TR58 and TS82 (6.5 cM apart). The genetic distance between foc5 and QTLAR3 peak was around 24 cM including six markers within this interval. The markers linked to both loci could facilitate the pyramiding of resistance genes for both diseases through MAS.

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Acknowledgements

This work has been supported by the European Union project: GLIP, contract no. FOOD-CT-2004-50622, and the national project : INIA, contract no. RTA04-067. M. Iruela acknowledges grant support from IFAPA-CICE Junta de Andalucia (Spain).

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Correspondence to J. Gil.

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Iruela, M., Castro, P., Rubio, J. et al. Validation of a QTL for resistance to ascochyta blight linked to resistance to fusarium wilt race 5 in chickpea (Cicer arietinum L.). Eur J Plant Pathol 119, 29–37 (2007). https://doi.org/10.1007/s10658-007-9121-0

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