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Phenotypic and histological expression of different genetic backgrounds in interactions between lettuce, wild Lactuca spp., L. sativa × L. serriola hybrids and Bremia lactucae

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Abstract

Phenotypic and histological responses of cultivated lettuce (Lactuca sativa) and wild relatives L. saligna, L.␣virosa as well as interspecific crosses derived from L. sativa × L. serriola to two races of Bremia lactucae (CS2, CS9) were investigated. With the exception of L. sativa genotypes, all accessions and hybrids expressed incomplete or complete resistance to both pathogen races, with slight differences at seedling and adult plant stages, respectively. Histological features of the interactions (development of pathogen infection structures and host hypersensitive response to attempted infection) were studied on leaf discs 48 h after inoculation. Interactions with similar phenotypic expression of resistance were characterized by significant variation in rate of development of pathogen infection structures and hypersensitive reactions. Differences found within eight Lactuca spp. accessions and hybrids challenged by two distinct pathogen races are interpreted and discussed.

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Abbreviations

dai:

days after inoculation

hai:

hours after inoculation

HR:

hypersensitive reaction

ID:

infection degree

IH:

intercellular hyphae

IMD:

irreversible membrane damage

IS:

infection site

ITRH:

intracellular hyphae

ITERH:

intercellular hyphae

NC:

number of necrotic epidermal cells per infection site

PV:

primary vesicle

SV:

secondary vesicle

SEN:

subepidermal necrosis

PN:

proportion of infection sites with necrotic epidermal cells

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Acknowledgements

A. Lebeda performed part of the research at HRI (Wellesbourne, UK) as a visiting scientist funded by Horticultural Research Association (Wellesbourne, UK). The work was finished during the stay of M. Sedlářová at HRI supported by post-doc grant GAČR 522/02/D011. Support of a further grant Variability of components and interactions in plant pathosystem and impact of environmental factors on their expression (MSM 6198959215) is greatly acknowledged. D. Pink’s research is funded by the UK Department of the Environment, Food and Rural Affairs (Defra).

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Authors and Affiliations

  1. Faculty of Science, Department of Botany, Palacký University, Šlechtitelů 11, 783 71, Olomouc-Holice, Czech Republic

    Aleš Lebeda & Michaela Sedlářová

  2. Warwick HRI, University of Warwick, Wellesbourne, Warwick, CV35 9EF, UK

    James Lynn & David A. C. Pink

Authors
  1. Aleš Lebeda
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  2. Michaela Sedlářová
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  3. James Lynn
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  4. David A. C. Pink
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Correspondence to Aleš Lebeda.

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Lebeda, A., Sedlářová, M., Lynn, J. et al. Phenotypic and histological expression of different genetic backgrounds in interactions between lettuce, wild Lactuca spp., L. sativa × L. serriola hybrids and Bremia lactucae . Eur J Plant Pathol 115, 431–441 (2006). https://doi.org/10.1007/s10658-006-9034-3

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