Damping-off and stem rot disease-causing Sclerotium rolfsii has been reported as a destructive soil-borne pathogen of numerous crops, especially in the tropics and subtropics. Trials were conducted to test the efficacy of biocontrol agents alone or combined with Moringa oleifera leaf extracts for the control of the disease. In the laboratory, PDA was amended with Moringa leaf extract, and mycelial growth of S. rolfsii was measured. In the greenhouse and field, Trichoderma Kd 63, Trichoderma IITA 508 and Bacillus subtilis were evaluated as seed treatments, soil drench or sprinkle, separately or combined with Moringa leaf extracts. Percentage disease incidence, severity and control were recorded. In the laboratory, the higher the extract concentration the less the mycelial growth and no mycelial growth occurred on extract at 15 or 20 g leaves 10 ml−1 water. In the greenhouse, the highest disease control was observed at a Moringa extract concentration of 15 kg leaves 10 l−1 water (w/v). Seed treatments using Trichoderma Kd 63, and soil sprinkle using Trichoderma IITA 508 had a significantly (P = 0.05) higher effect on a disease incidence than Bacillus. Disease severity followed the same pattern. Moringa seed treatment combined with Trichoderma soil sprinkle resulted in significantly more than 94% and 70% disease control in the greenhouse and field, respectively, with significant yield increase in the field. This is the first report of Moringa leaf extract combined with Trichoderma as an integrated control for Sclerotium damping-off and stem rot of cowpea in the field.
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Adandonon A, Aveling TAS, Labuschagne N, Ahohuendo BC, (2003) Epidemiology and biological control of the causal agent of damping-off and stem rot of cowpea in the Ouémé valley, Bénin Annales des Sciences Agonomiques du Bénin 6: 21–36
Asaka O, Shoda M, (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14 Applied and Environmental Microbiology 62: 4081–4085
Campbell R (1989) Biological Control of Microbial Plant Pathogens Cambridge University Press Cambridge, UK
Davis K (2000) The Moringa Tree. http://www.tropical-seeds.com/tech_forum/fruits_anon/moringa_tree.html
Demoz BT, Korsten L, (2006) Bacillus subtilis attachment, colonization, and survival on avocado Xowers and its mode of action on stem-end rot pathogens Biological Control 37: 68–74
Govender V, Korsten L, (2006) Evaluation of different formulations of Bacillus licheniformis in mango pack house trials Biological Control 37: 237–242
Hornby D (1990) Biological control of soil-borne plant pathogens CAB International Wallingford, UK
Howell CR (2003) Mechanisms employed by Trichoderma sepecies in the biological control of plant diseases: The history and evolution of current concepts Plant Disease 87: 4–10
Howell CR, DeVay JE, Garber RH, Batson WE, (1997) Field control of cotton seedling diseases with Trichoderma virens in combination with fungicide seed treatments Journal of Cotton Science 1: 15–20
Jacobsen BJ, Zidack NK, Larson BJ, (2004) The role of Bacillus-based biological control agents in integrated pest management systems: Plant diseases Phytopathology 94: 1272–1275
Lewis JA, Papavizas GC, Hollenbeck MD, (1993) Biological control of damping-off of snapbeans caused by Sclerotium rolfsii in the greenhouse and field with formulations of Gliocladium virens Biological Control 3(2): 109–115
Madi L, Katan T, Katan J, Henis Y, (1997) Biological control of Sclerotium rolfsii and Verticillium dahliae by Talaromyces flavus is mediated by different mechanisms Phytopathology 87: 1054–1060
McLean KL, Swaminathan J, Frampton CM, Hunt JS, Ridgway HJ, Stewart A, (2005) Effect of formulation on the rhizosphere competence and biocontrol ability of Trichoderma atroviride C52 Plant Pathology 54: 212–218
Mukherjee PK, Raghu K, (1997) Effect of temperature on antagonistic and biocontrol potential of Trichoderma sp. on Sclerotium rolfsii Mycopathologia 139: 151–155
Obagwu J, Korsten L, (2003) Control of citrus and blue moulds with garlic extracts European Journal of Plant Pathology 109: 221–225
Obagwu J, Emechebe AM, Adeoti AA, (1997) Effect of extracts of garlic (Allium sativum L.) bulb and neem (Azadirachta indica Juss) seed on the mycelium growth and sporulation of Colletotrichum capsici Journal of Agricultural Technology 5: 51–55
Papavizas GC, Lewis JA, Abd-El Moity TH, (1982) Evaluation of new biotypes of Trichoderma harzianum for tolerance to benomyl and enhanced bicontrol capabilities Phytopathology 72: 126–132
Punja ZK (1985) The biology, ecology and control of Sclerotium rolfsii Annual Review of Phytopathology 23: 97–127
SAS (1997) SAS Institute Inc., SAS/STAT Software: Changes and Enhancements through Release 6.12. Cary, North Carolina
SIBAT (1993) Organic pest control in rice, corn and vegetables. Techno-Series 1, Quezon City, Phillipines
Stoll G (1988) Protection Naturelle des Végétaux en Zone Tropicale (Natural Protection of Plants in Tropical Zone). Eds Margraf Verlag., CTA, AGRECOL
Tu JC, (1997) An integrated control of white mold (Sclerotinia sclerotiorum) of beans, with emphasis on recent advances in biological control Botanical Bulletin of Academia Sinica 38: 73–76
Weideman H, Wehner FC, (1993) Greenhouse evaluation of Trichoderma harzianum and Fusarium oxysporum for biological control of citrus root rot in soils naturally and artificially infected with Phytophthora nicotianae Phytophylactica 25: 101–105
Widyastuti SM, Sumardi H, Yuniarti D, (2003) Biological control of Sclerotium rolfsii damping-off of tropical pine (Pinus merkusii) with three isolates of Trichoderma spp Journal of Biological Sciences 3(1): 95–102
Wokocha RC, (1990) Integrated control of Sclerotium rolfsii infection of tomato in the Nigerian Savanna: effect of Trichoderma viride and some fungicides Crop Protection 9: 231–234
Wulff EG, van Vuurde JWL, Hockenhull J, (2003) The ability of the biological control agent Bacillus subtilis, strain BB, to colonise vegetable brassicas endophytically following seed inoculation Plant Soil 255: 463–474
Research was financed by the International Institute of Tropical Agriculture (IITA), Nigeria. Dr M. Morris, Plant Health Products, Pietermaritzburg and Prof P. L. Steyn, Stimuplant CC, Mooiplaats, Pretoria, South Africa kindly provided Trichoderma Kd 63 and Bacillus subtilis, respectively.
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Adandonon, A., Aveling, T., Labuschagne, N. et al. Biocontrol agents in combination with Moringa oleifera extract for integrated control of Sclerotium-caused cowpea damping-off and stem rot. Eur J Plant Pathol 115, 409–418 (2006). https://doi.org/10.1007/s10658-006-9031-6
- Bacillus subtilis
- integrated control
- Moringa oleifera
- Sclerotium rolfsii
- Trichoderma harzianum
- Vigna unguiculata