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Plant extracts containing caffeic acid and rosmarinic acid inhibit zoospore germination of Phytophthora spp. pathogenic to Theobroma cacao

  • Timothy L. WidmerEmail author
  • Nathalie Laurent
Article

Abstract

The three most important species of Phytophthora worldwide causing black pod disease of cacao are P. palmivora, P. megakarya, and P. capsici. Chemicals are effective in controlling this disease but more natural methods would be preferred. One alternative is to use natural plant extracts. Rosemary and lavender leaf extracts were found to be effective in reducing germination of P. capsici, P. megakarya, and P. palmivora zoospores when supplemented to agar plates at different dilutions. The extracts displayed the biggest impact on P. megakarya zoospores where it completely inhibited germination at a 25% dilution of the prepared extract. When applied to cacao leaf disks, rosemary extract reduced necrosis caused by P. megakarya zoospores. In a bioassay, pears first treated with lavender extract showed no symptoms of P. megakarya infection compared with the non-treated controls. Based upon HPLC analyses, the active compound in these extracts was determined to be caffeic acid, rosmarinic acid or some simple derivative thereof. When added to agar plates, synthetic caffeic acid and rosmarinic acid completely inhibited germination of P. capsici, P. megakarya, and P. palmivora zoospores at concentrations of 3 and 6 g l−1, respectively. In addition, sage and rice bran extracts, which both contain caffeic acid, were also effective in reducing zoospore germination. In contrast, inhibition of Botrytis cinerea or Trichoderma asperellum conidia germination did not occur, displaying some level of specificity. These extracts could provide an economically safe method for reducing damage caused by black pod disease on cacao until resistant varieties are developed and released.

Keywords

cacao cinnamic acid lavender natural products rosemary sage 

References

  1. Alam S (2004) Synthesis, antibacterial and antifungal activity of some derivatives of 2-phenyl-chromen-4-one. Journal of Chemistry Science 116:325–331CrossRefGoogle Scholar
  2. Asahina Y, Iwase K, Iinuma F, Hosaka M, Ishizaki T (2005) Synthesis and antibacterial activity of 1-(2-fluorovinyl)-7-substituted-4-quinolone-3-carboxylic acid derivatives, conformationally restricted analogues of fleroxacin. Journal of Medicinal Chemistry 48:3194–3202PubMedCrossRefGoogle Scholar
  3. Bais HP, Walker TS, Schweizer HP, Vivanco JM (2002) Root specific elicitation and antimicrobial activity of rosmarinic acid in hairy root cultures of Ocimum basilicum. Plant Physiolology and Biochemistry 40:983–995CrossRefGoogle Scholar
  4. Basile A, Jiménez-Carmona MM, Clifford AA (1998) Extraction of rosemary by superheated water. Journal of Agricultural and Food Chemistry 46:5205–5209CrossRefGoogle Scholar
  5. Bateman RP, Hidalgo E, Garcia J, Arroyo C, ten Hoopen GM, Adonijah V, Krauss U (2005) Application of chemical and biological agents for the management of frosty pod rot (Moniliophthora roreri) in Costa Rican cocoa (Theobroma cacao). Annals of Applied Biology 147:129–138CrossRefGoogle Scholar
  6. Bowers JH, Bailey BA, Hebbar PK, Sanogo S and Lumsden RD (2001) The impact of plant diseases on world chocolate production. Plant Health Progress Online [http://www.apsnet.org/online/feature/cacao/]Google Scholar
  7. Brasier CM, Griffin M (1979) Taxonomy of Phytophthora palmivora on cocoa. Transactions of the British Mycological Society 72:111–143CrossRefGoogle Scholar
  8. Dakwa JT (1984) Nationwide black pod survey: Joint CRIG/Cocoa Production Division Project. Annual Report, 1976/77–1978/79. Cocoa Research Institute of Ghana, Tafo, Ghana, p. 263Google Scholar
  9. Dakwa JT (1987) A serious outbreak of black pod disease in a marginal area of Ghana. Proceedings of the Xth International Cocoa Research Conference, Santo Domingo, Dominican Republic (pp. 447–452) Cocoa Producers’ Alliance, Lagos, NigeriaGoogle Scholar
  10. Debersac P, Vernevaut M-F, Amiot M-J, Suschetet M, Siess M-H (2001) Effects of a water-soluble extract of rosemary and its purified component rosmarinic acid on xenobiotic-metabolizing enzymes in rat liver. Food and Chemical Toxicology 39:109–117PubMedCrossRefGoogle Scholar
  11. Duguma B, Gockowski J, Bakala J (2001) Smallholder cacao (Theobroma cacao Linn.) cultivation in agroforestry systems of West and Central Africa: Challenges and opportunities. Agroforestery Systems 51:177–188CrossRefGoogle Scholar
  12. Henderson CP, Asante EG, Donkor MA, Ameyaw KO, Luterbacher MC, Akrofi AY, Boakye PD (1994) Farmer managed fungicide trials (Volta and Brong Ahafo Regions). Annual Report of the Cocoa Research Institute, Ghana, 1991/92:70–76Google Scholar
  13. Hislop EC, Park PO (1960) Fungicide research. Annual Report of the West African Cocoa Research Institute 61:96–106Google Scholar
  14. Holmes KA, Schroers HJ, Thomas SE, Evans HC, Samuels GJ (2004) Taxonomy and biocontrol potential of a new species of Trichoderma from the Amazon basin of South America. Mycological Progress 3:199–210CrossRefGoogle Scholar
  15. Ibanez E, Kubatova A, Senorans FJ, Cavero S, Reglero G, Hawthorne SB (2003) Subcritical water extraction of antioxidant compounds from rosemary plants. Journal of Agricultural and Food Chemistry 51:375–382PubMedCrossRefGoogle Scholar
  16. Iwaro AD, Sreenivasan TN, Umaharan P (1997) Foliar resistance to Phytophthora palmivora as an indicator of pod resistance in Theobroma cacao. Plant Disease 81:619–624CrossRefGoogle Scholar
  17. Iwaro AD, Sreenivasan TN, Umaharan P (1998) Cacao resistance to Phytophthora: Effect of pathogen species, inoculation depths and pod maturity. European Journal of Plant Pathology 104:11–15CrossRefGoogle Scholar
  18. Kovatcheva E, Pavlov A, Koleva I, Ilieva M, Mihneva M (1996) Rosmarinic acid from Lavandula vera MM cell culture. Phytochemistry 43:1243–1244CrossRefGoogle Scholar
  19. Kurihara N, Miyamoto J, Paulson GD, Zeeh B, Skidmore MW, Hollingworth RM, Kuiper JA (1997) Chirality in synthetic agrochemicals: Bioactivity and safety consideration. Pure and Applied Chemistry 69:1335–1348CrossRefGoogle Scholar
  20. Len C, Boulogn-Merlot AS, Postel D, Ronco G, Villa P (1996) Synthesis and antifungal activity of novel bis(dithiocarbamate) derivatives of glycerol. Journal of Agricultural and Food Chemistry 44:2856–2858CrossRefGoogle Scholar
  21. Mitchell DJ, Kannwischer-Mitchell ME (1992) Phytophthora. In: Singleton LL, Mihail JD, Rush CM (eds) Methods for Research on Soilborne Phytopathogenic Fungi. The American Phytopathological Society Press, St. Paul, MN, pp. 31–38Google Scholar
  22. Nair RB, Xia Q, Kartha CJ, Kurylo E, Hirji RN, Datla R, Selvaraj G (2002) Arabidopsis CYP98A3 mediating aromatic 3-hydroxylation. Developmental regulation of the gene, and expression in yeast. Plant Physiology 130:210–220PubMedCrossRefGoogle Scholar
  23. Nascimento GGF, Locatelli J, Freitas PC, Silva GL (2000) Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology 31:247–256Google Scholar
  24. Nyassé S, Despréaux D, Cilas C (2002) Validity of a leaf inoculation test to assess the resistance to Phytophthora megakarya in a cocoa (Theobroma cacao L.) diallel mating design. Euphytica 123:395–399CrossRefGoogle Scholar
  25. Opoku IY, Akrofi AY and Afrifa AA (1997) The spread of Phytophthora megakarya on cocoa in Ghana. Proceedings 1st International Cocoa Pests and Diseases Seminar, 6–10 Nov, 1995 (pp. 98–107) Accra, GhanaGoogle Scholar
  26. Opoku IY, Appiah AA, Akrofi AY (2000) Phytophthora megakarya: A potential threat to the cocoa industry in Ghana. Ghana Journal of Agricultural Science 33:135–142Google Scholar
  27. Ravn H, Andary C, Kovacs G, Molgaard P (1989) Caffeic acid esters as in vitro inhibitors of plant pathogenic bacteria and fungi. Biochemical Systematics and Ecology 17:175–184CrossRefGoogle Scholar
  28. Reddy KN, Singh M (1992) Organosilicone adjuvant effects on glyphosphate efficacy and rainfastness. Weed Technology 6:361–365Google Scholar
  29. Roggenbuck FC, Rowe L, Penner D, Petroff L, Burrow R (1990) Increasing postemergence herbicide efficacy and rainfastness with silicone adjuvants. Weed Technology 4:576–580Google Scholar
  30. Sanbongi C, Osakabe N, Natsume M, Takizawa T, Gopmi S, Osawa T (1998) Antioxidative polyphenols isolated from Theobroma cacao. Journal of Agricultural and Food Chemistry 46:454–457PubMedCrossRefGoogle Scholar
  31. Shetty K (1997) Biotechnology to harness the benefits of dietary phenolics; focus on Lamiaceae. Asia Pacific Journal of Clinical Nutrition 6:162–171Google Scholar
  32. Sun J, Foy CL, Witt HL (1996) Effect of organosilicone surfactants on the rainfastness of primisulfuron in velvetleaf (Abutilon threophrasti). Weed Technology 10:263–267Google Scholar
  33. Tahi M, Kebe I, Eskes AB, Ouattara S, Sangare A, Mondeil F (2000) Rapid screening of cacao genotypes for field resistance to Phytophthora palmivora using leaves, twigs and roots. European Journal of Plant Pathology 106:87–94CrossRefGoogle Scholar
  34. Vinson JA, Stella JM, Flanagan TJ (1998) Selenium yeast is an effective in vitro and in vivo antioxidant and hypolipemic agent in normal hamsters. Nutrition Research 18:735–742CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, B.V. 2006

Authors and Affiliations

  1. 1.European Biological Control LaboratoryUSDA/ARSSt. Gely du FescFrance
  2. 2.Foreign Disease and Weed Science Research UnitUSDA-ARSFrederickUSA

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