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Occurrence, isolation and biological activity of phytotoxic metabolites produced in vitro by Sphaeropsis sapinea, pathogenic fungus of Pinus radiata

Abstract

Sphaeropsis sapinea was repeatedly isolated in Sardinia from symptomatic samples of the upper part of declining pine (Pinus radiata) plants. Observed symptoms mainly consisted of foliage chlorosis, drying of needles and cankers on branches. The S. sapinea strains were shown to produce phytotoxic metabolites in culture filtrates. Three metabolites were isolated for the first time from this fungus and identified by their spectroscopic and optical properties as R-(−)-mellein, (3R,4R)-4-hydroxymellein and (3R,4S)-4-hydroxymellein. When assayed for phytotoxic and antifungal activities on host and non-host plants and on some phytopathogenic fungi, the R-(−)-mellein showed significant activity, while the other two 3,4-dihydroisocoumarins showed only a synergic activity in both tests.

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Acknowledgements

The authors thank the Servizio di Spettrometria di Massa del CNR e dell’Università di Napoli Federico II (the assistance of the staff is gratefully acknowledged) and Dr. Lia Ferracane for EI and ESI mass spectra and CIMCF of Università di Napoli Federico II for NMR spectra. This work was supported by a grant from the Italian Ministry of University and Research (MIUR). Contribution DISSPA 102.

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Correspondence to Antonio Evidente.

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Cabras, A., Mannoni, M.A., Serra, S. et al. Occurrence, isolation and biological activity of phytotoxic metabolites produced in vitro by Sphaeropsis sapinea, pathogenic fungus of Pinus radiata . Eur J Plant Pathol 115, 187 (2006). https://doi.org/10.1007/s10658-006-9006-7

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  • DOI: https://doi.org/10.1007/s10658-006-9006-7

Keywords

  • declining pine disease
  • isocoumarines
  • melleins
  • phytotoxic and antifungal activity
  • Pinus radiata
  • Sphaeropsis sapinea