Skip to main content
SpringerLink
Log in

Identification and Partial Characterisation of Lettuce big-vein associated virus and Mirafiori lettuce big-vein virus in Common Weeds Found Amongst Spanish Lettuce Crops and their Role in Lettuce Big-vein Disease Transmission

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

The potential role of 10 frequently occurring weed species found amongst Spanish lettuce crops as host plants for the two viruses associated with the lettuce big-vein disease, Lettuce big-vein associated virus (LBVaV) and Mirafiori lettuce big-vein virus (MLBVV), was studied. The results showed that both viruses can infect naturally growing Sonchus oleraceus (common sowthistle) plants, the unique susceptible species detected among the analysed weeds. The sequences of the coat protein (CP) genes of the LBVaV and MLBVV isolates recovered from S. oleraceus plants were determined. Phylogenetic studies revealed a very close relationship between the CP sequences from these weed isolates and those from Spanish lettuce. Moreover, we showed that S. oleraceus can act as a source of lettuce infection by means of Olpidium brassicae, the vector fungus of both viruses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • N Astruc JF Marcos G Macquaire T Candresse V Pallas (1996) ArticleTitleStudies on the diagnosis of hop stunt viroid in fruit trees: Identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents European Journal of Plant Pathology 102 837–846 Occurrence Handle10.1007/BF01877053 Occurrence Handle1:CAS:528:DyaK2sXkslOrtA%3D%3D

    Article  CAS  Google Scholar 

  • Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L and Zurcher EJ (eds) (1997). Plant Viruses Online: Descriptions and Lists from the VIDE Database. URL http://image.fs.uidaho.edu/vide/refs.htm.

  • RN Campbell (1963) ArticleTitleWeed hosts of Olpidium brassicae and the Lettuce big-vein virus Phytopathology 53 872

    Google Scholar 

  • RN Campbell (1965) ArticleTitleWeeds as reservoir hosts of the Lettuce big-vein virus Canadian Journal of Botany 43 1141–1149

    Google Scholar 

  • RN Campbell (1996) ArticleTitleLettuce ring necrosis, a virus-like disease of lettuce: Evidence for transmission by Olpidium brassicae Plant Disease 80 611–615 Occurrence Handle10.1094/PD-80-0611

    Article  Google Scholar 

  • RN Campbell ST Sim (1994) ArticleTitleHost specificity and nomenclature of Olpidium bornovanus (=radicale) and comparisons to Olpidium brassicae Canadian Journal of Botany 72 1136–1143 Occurrence Handle10.1139/b94-139

    Article  Google Scholar 

  • S Dellaporta H Woods J Hicks (1983) ArticleTitleA plant DNA mini-preparation: version II Plant Molecular Biology Reporter 1 19–21 Occurrence Handle1:CAS:528:DyaL2cXksFWhtrk%3D Occurrence Handle10.1007/BF02712670

    Article  CAS  Google Scholar 

  • IC Jagger N Chandler (1934) ArticleTitleBig vein, a disease of lettuce Phytopathology 24 1253–1256

    Google Scholar 

  • RA Jones (2004) ArticleTitleUsing epidemiological information to develop effective integrated virus disease management strategies Virus Research 100 5–30 Occurrence Handle10.1016/j.virusres.2003.12.011 Occurrence Handle15036832 Occurrence Handle1:CAS:528:DC%2BD2cXht1yrsL8%3D

    Article  PubMed  CAS  Google Scholar 

  • Y Kawazu T Sasaya T Morikawa K Sugiyama T Natsuaki (2003) ArticleTitleNucleotide sequence of the coat protein gene of Mirafiori lettuce virus Journal of General Plant Pathology 69 55–60 Occurrence Handle10.1007/s10327-002-0019-6 Occurrence Handle1:CAS:528:DC%2BD3sXjtVKktrg%3D

    Article  CAS  Google Scholar 

  • G Kobs (1997) ArticleTitleCloning Blunt-End DNA Fragments into the pGEM®-T Vector Systems Promega Notes Magazine 62 15.

    Google Scholar 

  • S Kumar K Tamura IB Jakobsen M Nei (2001) ArticleTitleMEGA2: Molecular Evolutionary Genetics Analysis software Bioinformatics 17 1244–1245 Occurrence Handle10.1093/bioinformatics/17.12.1244 Occurrence Handle11751241 Occurrence Handle1:CAS:528:DC%2BD38XmtVCktQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • H Lot RN Campbell S Souche RG Milne P Roggero (2002) ArticleTitleTransmission by Olpidium brassicae of Mirafiori lettuce virus and Lettuce big-vein virus, and their roles in lettuce big-vein etiology Phytopathology 92 288–293 Occurrence Handle18944001

    PubMed  Google Scholar 

  • JA Navarro F Botella A Maruhenda P Sastre MA Sánchez-Pina V Pallas (2004) ArticleTitleComparative infection progress analysis of Lettuce big-vein virus and Mirafiori lettuce virus in lettuce crops by developed molecular diagnosis techniques Phytopathology 94 470–477 Occurrence Handle1:CAS:528:DC%2BD2cXkt1Sitrw%3D Occurrence Handle18943765

    CAS  PubMed  Google Scholar 

  • JA Navarro VA Torok HJ Vetten V Pallas (2005) ArticleTitleGenetic variability in the coat protein genes of Lettuce big-vein-associated virus and Mirafiori lettuce big-vein virus Archives of Virology 150 681–694 Occurrence Handle10.1007/s00705-004-0456-y Occurrence Handle15614432 Occurrence Handle1:CAS:528:DC%2BD2MXisV2hsbo%3D

    Article  PubMed  CAS  Google Scholar 

  • P Roggero M Ciuffo AM Vaira GP Accotto V Masenga RG Milne (2000) ArticleTitleAn Ophiovirus isolated from lettuce with big-vein symptoms Archives of Virology 145 2629–2642 Occurrence Handle10.1007/s007050070012 Occurrence Handle11205109 Occurrence Handle1:STN:280:DC%2BD3M7hvFKnsQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • P Roggero H Lot S Souche R Lenzi RG Milne (2003) ArticleTitleOccurrence of Mirafiori lettuce virus and Lettuce big-vein virus in relation to development of big-vein symptoms in lettuce crops European Journal of Plant Pathology 109 261–267 Occurrence Handle10.1023/A:1023060830841 Occurrence Handle1:CAS:528:DC%2BD3sXit1Kisb4%3D

    Article  CAS  Google Scholar 

  • J Sambrook EF Fritsch T Maniatis (1989) Molecular Cloning: A Laboratory Manual EditionNumber2 Cold Spring Harbour Laboratory Press New York

    Google Scholar 

  • JA Sánchez-Navarro F Aparicio A Rowhani V Pallás (1998) ArticleTitleComparative analysis of ELISA, nonradioactive molecular hybridization and PCR for the detection of Prunus necrotic ringspot virus in herbaceous and Prunus hosts Plant Pathology 47 780–786

    Google Scholar 

  • JA Sánchez-Navarro MC Cañizares EA Cano V Pallás (1999) ArticleTitleSimultaneous detection of five carnation viruses by non-isotopic molecular hybridization Journal of Virological Methods 82 167–175 Occurrence Handle10894633

    PubMed  Google Scholar 

  • T Sasaya K Ishikawa H Koganezawa (2001) ArticleTitleNucleotide sequence of the coat protein gene of Lettuce big-vein virus Journal of General Virology 82 1509–1515 Occurrence Handle11369898 Occurrence Handle1:CAS:528:DC%2BD3MXkt1Cht7g%3D

    PubMed  CAS  Google Scholar 

  • T Sasaya K Ishikawa H Koganezawa (2002) ArticleTitleThe nucleotide sequence of RNA1 of Lettuce big-vein virus, genus Varicosavirus reveals its relation to nonsegmented negative-strand RNA viruses Virology 297 289–297 Occurrence Handle10.1006/viro.2002.1420 Occurrence Handle12083827 Occurrence Handle1:CAS:528:DC%2BD38Xksl2ntLs%3D

    Article  PubMed  CAS  Google Scholar 

  • T Sasaya S Kusaba K Ishikawa H Koganezawa (2004) ArticleTitleNucleotide sequence of RNA 2 of Lettuce big-vein virus and evidence for a possible transcription termination/initiation strategy similar to that of rhabdoviruses Journal of General Virology 85 2709–2717 Occurrence Handle10.1099/vir.0.80061-0 Occurrence Handle15302964 Occurrence Handle1:CAS:528:DC%2BD2cXnsVegsL8%3D

    Article  PubMed  CAS  Google Scholar 

  • JHM Temmink RN Campbell PR Smith (1970) ArticleTitleSpecificity and site of in vitro acquisition of Tobacco necrosis virus by zoospores of Olpidium brassicae Journal of General Virology 9 201–213

    Google Scholar 

  • JD Thompson TJ Gibson F Plewniak F Jeanmougin DG Higgins (1997) ArticleTitleThe ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Research 24 4876–4882

    Google Scholar 

  • F Wilk Particlevan der AM Dullemans M Verbeek JF Heuvel Particlevan den (2002) ArticleTitleNucleotide sequence and genomic organization of an ophiovirus associated with lettuce big-vein disease Journal of General Virology 83 2869–2877 Occurrence Handle12388823

    PubMed  Google Scholar 

  • HJ Vetten DE Lesemann J Dalchow (1987) ArticleTitleElectron microscopical and serological detection of virus-like particles associated with lettuce big-vein disease Journal of Phytopathology 120 53–59

    Google Scholar 

  • JA Walsh (1994) ArticleTitleEffects of some biotic and abiotic factors on symptom expression of Lettuce big-vein virus in lettuce (Lactuca sativa) The Journal of Horticultural Science 69 21–28

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. UPV-CSIC, Instituto de Biología Molecular y Celular de Plantas (IBMCP), Avda. dels Tarongers, s/n, 46022, Valencia, Spain

    Jose A. Navarro & Vicente Pallas

  2. Departamento de Biología Aplicada, Universidad Miguel Hernández, Avda. Ferrocarril, s/n, 03202, Elx, Spain

    Francisco Botella

  3. PRIMAFLOR S.A.T., La Estacion 2, Almeria, Spain

    Antonio Marhuenda & Pedro Sastre

  4. Centro de Edafología y Biología Aplicada del Segura (CEBAS), CSIC, Campus Universitario de Espinardo, P.O. Box 164 30100, Murcia, Spain

    M. Amelia Sánchez-Pina

Authors
  1. Jose A. Navarro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Francisco Botella
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Antonio Marhuenda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pedro Sastre
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Amelia Sánchez-Pina
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vicente Pallas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vicente Pallas.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Navarro, J.A., Botella, F., Marhuenda, A. et al. Identification and Partial Characterisation of Lettuce big-vein associated virus and Mirafiori lettuce big-vein virus in Common Weeds Found Amongst Spanish Lettuce Crops and their Role in Lettuce Big-vein Disease Transmission. Eur J Plant Pathol 113, 25–34 (2005). https://doi.org/10.1007/s10658-005-8916-0

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-005-8916-0

Keywords

Search

Navigation