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Distribution of Mating Types and Diversity in Virulence of Didymella rabiei in Israel

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Abstract

The distribution of mating types and diversity in virulence of Didymella rabiei populations were studied in Israel from 1997 to 1999. Forty-one monoconidial D. rabiei isolates from 18 commercial fields distributed among all the chickpea production areas of the country were paired with MAT1-1 and MAT1-2 mating type tester isolates of D. rabiei. Both mating types were found in all chickpea production areas of the country. Of the 18 fields sampled, MAT1-1 was observed in 44%, and MAT1-2 in 88% of the sites. In some sites both mating types were present in close proximity, suggesting that sexual reproduction of the pathogen was feasible. The contribution of sexual reproduction of the fungus to virulence diversity was tested on detached leaves of six differential chickpea cultivars. Nine isolates were derived from different well separated foci (derived from ascospores as inoculum) and eight isolates were derived from a single, well defined infection focus (derived from sister conidia). In the analyses of variance the cultivar × isolate interaction showed no significant (P of F>0.09) effect on disease incidence; the chickpea cultivars differed significantly (P of F<0.0001) in their response to D. rabiei; and the isolate effect was highly significant (P of F = 0.0007) for the conidial population, but not significant (P of F>0.1) among isolates of the ascosporic population. Nevertheless, when comparing a cultivar at a time, the ascosporic and conidial populations did not differ significantly (P of F>0.1) in their virulence diversity. Virulence of 41 isolates collected from the different chickpea fields was tested on detached leaves of four Israeli cultivars that differ in their field response to D. rabiei. The cultivar × isolate interaction showed no significant effect (P of F = 0.95) on disease incidence. The main effects of cultivar and isolate on disease incidence were highly significant (P of F<0.0001). Accordingly, our data do not support the hypothesis that there is pathogenic specialization in the D. rabiei–C. arietinum pathosystem in Israel.

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References

  • GN Agrios (1997) Genetics of plant disease GN Agrios (Eds) Plant Pathology EditionNumber4 Academic Press San Diego USA 115–142

    Google Scholar 

  • DJ Allen (1983) The Pathology of Tropical Food Legumes Wiley Chichester UK

    Google Scholar 

  • MP Barve T Arie SS Salimath FJ Muehlbauer TL Peever (2003) ArticleTitleCloning and characterization of the mating type (MAT) locus from Ascochyta rabiei (teleomorph: Didymella rabiei) and a MAT phylogeny of legume-associated Ascochyta spp Fungal Genetics and Biology 39 151–167 Occurrence Handle10.1016/S1087-1845(03)00015-X Occurrence Handle12781674 Occurrence Handle1:CAS:528:DC%2BD3sXktFGhs7o%3D

    Article  PubMed  CAS  Google Scholar 

  • R Bruns W Barz (2001) ArticleTitleStudies on cell number and nuclei in spores and on ploidy level in Ascochyta rabiei isolates Journal of Phytopathology 149 253–258 Occurrence Handle10.1046/j.1439-0434.2001.00613.x

    Article  Google Scholar 

  • FS Dolar A Tenuta VJ Higgins (1994) ArticleTitleDetached leaf assay for screening chickpea for resistance to Ascochyta blight Canadian Journal of Plant Pathology 16 215–220 Occurrence Handle10.1080/07060669409500756

    Article  Google Scholar 

  • Gamliel E, Shtienberg D, Vintal H, Fischer E and Dinoor A (1999) Prevalence, maturation and dissemination of Didymella rabiei, the teleomorph of Ascochyta blight. In: Israel XIV International Plant Protection Congress Jerusalem (pp 165) Israel.

  • MV Gorlenko LN Bushkova (1958) ArticleTitlePerfect state of the causal agent of ascochytosis of chickpea Review of Applied Mycology 37 695

    Google Scholar 

  • MP Haware (1987) ArticleTitleOccurrence of the perfect state of Ascochyta rabiei in Syria International Chickpea Newsletter 17 29–30

    Google Scholar 

  • H Jan MV Wiese (1991) ArticleTitleVirulence forms of Ascochyta rabiei affecting chickpea in the Palouse Plant Disease 75 904–906 Occurrence Handle10.1094/PD-75-0904

    Article  Google Scholar 

  • Jiménez-Díaz RM, Navas-Cortés JA and Trapero-Casas A (1987) Occurrence of Mycosphaerella rabiei, the teleomorph of Ascochyta rabiei in Andalucía. In: Congress of the Mediterranean Phytopathological Society, Union Granada (pp 124–125) Spain.

  • Kaiser WJ (1992) Epidemiology of Ascochyta rabiei. In: Singh KB and Saxena MC (eds) Disease Resistance Breeding in Chickpea–Proceedings of the Consultative Meeting on Breeding for Disease Resistance in Kabuli Chickpea ICARDA–International Center for Agricultural Research in the Dry Areas Aleppo (pp 117–134) Syria.

  • WJ Kaiser RM Hannan (1987) ArticleTitleFirst report of Mycosphaerella rabiei on chickpeas in the Western hemisphere Plant Disease 71 192

    Google Scholar 

  • WJ Kaiser I Küsmenoglu (1997) ArticleTitleDistribution of mating types and the teleomorph of Ascochyta rabiei on chickpea in Turkey Plant Diseae 81 1284–1287

    Google Scholar 

  • Kovachevski IC (1936) The blight of chickpea (Cicer arietinum), Mycosphaerella rabiei f. sp., Report Number 15:700. Ministry of Agriculture and National Domains, Plant Protection Institute Sofia, Bulgaria.

  • G Kövics L Holly EI Simay (1986) ArticleTitleAn ascochytosis of the chickpea (Cicer arietinum L.) caused by Didymella rabiei (Kov.) v. Arx: imperfect Ascochyta rabiei (Pass.) Lab. in Hungary Acta Phytopathollogy and Entomollogy of Hungary 21 147–150

    Google Scholar 

  • J Lichtenzveig P Winter S Abbo D Shtienberg WJ Kaiser J Kahl (2002) ArticleTitleTowards the first linkage map of the Didymella rabiei genome Phytoparasitica 30 467–472 Occurrence Handle1:CAS:528:DC%2BD38XosFaksb4%3D

    CAS  Google Scholar 

  • MG Milgroom TL Peever (2003) ArticleTitlePopulation biology of plant pathogens: the synthesis of plant disease epidemiology and population genetics Plant Disease 87 608–617

    Google Scholar 

  • JA Navas-Cortés E Perez-Artes RM Jiménez-Díaz A Llobel BW Bainbridge JB Heale (1998) ArticleTitleMating type, pathotype and RAPDs analysis in Didymella rabiei, the agent of Ascochyta blight in chickpea Phytoparasitica 26 199–212

    Google Scholar 

  • MV Reddy S Kabbabeh (1985) ArticleTitlePathogenic variability in Ascochyta rabiei (Pass.) Lab. in Syria and Lebanon Phytopathologia Mediterranea 24 265–266

    Google Scholar 

  • MV Reddy KB Singh YL Nene (1981) Screening techniques for Ascochyta blight of chickpea MC Saxena KB Singh (Eds) Workshop on Ascochyta Blight and Winter Sowing of Chickpeas Martinus Nijhoff/Dr. W. Junk Publishers for ICARDA Aleppo, Syria

    Google Scholar 

  • G Shaner EL Stromberg GH Lacy KR Barker TP Pirone (1992) ArticleTitleNomenclature and concepts of pathogenicity and virulence Annual Review of Phytopathology 30 47–66 Occurrence Handle10.1146/annurev.py.30.090192.000403 Occurrence Handle18643770 Occurrence Handle1:STN:280:DC%2BD1cvms1erug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • D Shtienberg H Vintal S Brener B Retig (2000) ArticleTitleRational management of Didymella rabiei in chickpea by integration of genotype resistance and post infection application of fungicides Phytopathology 90 834–842 Occurrence Handle1:CAS:528:DC%2BD3cXmtFKjsbs%3D Occurrence Handle18944504

    CAS  PubMed  Google Scholar 

  • D Shtienberg H Vintal Y Nitzani S Brener B Retig (1998) ArticleTitleThe perfect stage of Ascochyta blight (Didymella rabiei): a new threat to chickpea production in Israel? Phytoparasitica 26 157

    Google Scholar 

  • A Trapero-Casas WJ Kaiser (1992) ArticleTitleDevelopment of Didymella rabiei, the teleomorph of Ascochyta rabiei, on chickpea straw Phytopathology 82 1261–1266

    Google Scholar 

  • A Trapero-Casas JA Navas-Cortés RM Jiménez-Díaz (1996) ArticleTitleAirborne ascospores of Didymella rabiei as a major primary inoculum for Ascochyta blight epidemics in chickpea crops in southern Spain European Journal of Plant Pathology 102 237–245 Occurrence Handle10.1007/BF01877962

    Article  Google Scholar 

  • SM Udupa F Weigand MC Saxena G. Kahl (1998) ArticleTitleGenotyping with RAPD and microsatellite markers resolves pathotype diversity in the Ascochyta blight pathogen of chickpea Theoretical and Applied Genetics 97 299–307 Occurrence Handle1:CAS:528:DyaK1cXlslais70%3D Occurrence Handle10.1007/s001220050899

    Article  CAS  Google Scholar 

  • S Vir JS Grewal (1974) ArticleTitlePhysiologic specialization in Ascochyta rabiei the causal organism of gram blight Indian journal of Phytopathology 27 355–360

    Google Scholar 

  • AD Wilson WJ Kaiser (1995) ArticleTitleCytology and genetics of sexual incompatibility in Didymella rabiei Mycologia 87 795–804

    Google Scholar 

  • DG Zachos CG Panagopoulos SA Makris (1963) ArticleTitleRecherches sur la biologie, l’epidemiologie et la lutte contre l’anthracnose du pois-chiche Annals of Institute Phytopathology Benaki 5 167–192

    Google Scholar 

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Authors and Affiliations

  1. Department of Field Crops, Vegetables and Genetics, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel

    J. Lichtenzveig, O. Frenkel, S. Michaelido & S. Abbo

  2. Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel

    E. Gamliel, O. Frenkel & D. Shtienberg

  3. Department of Genomics, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel

    A. Sherman

Authors
  1. J. Lichtenzveig
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  2. E. Gamliel
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  3. O. Frenkel
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  4. S. Michaelido
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  5. S. Abbo
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  6. A. Sherman
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  7. D. Shtienberg
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Correspondence to D. Shtienberg.

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Lichtenzveig, J., Gamliel, E., Frenkel, O. et al. Distribution of Mating Types and Diversity in Virulence of Didymella rabiei in Israel. Eur J Plant Pathol 113, 15–24 (2005). https://doi.org/10.1007/s10658-005-8914-2

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