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Resistance to Dicarboximide Fungicides in Stemphylium vesicarium of Italian Pear Orchards

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Abstract

Brown spot, caused by Stemphylium vesicarium, is the main fungal disease of pear in northern Italy where it may cause severe crop losses and it requires numerous fungicide applications. Monitoring work was performed by collecting fungal populations in Po valley between 1995 and 2003 in order to study the dicarboximides resistance already detected in the 1990s for procymidone as a result of control failures in field. Sensitivity tests showed that the resistant strains occur all over the monitored areas. Where present the efficacy of procymidone in field is completely lost in spite of what is observed often in other fungi. In most of the isolates (phenotype R1), S. vesicarium resistance level to procymidone (Sialex) was shown to be very high (RF≅3000) whereas it was lower towards the other dicarboximides iprodione (Rovral), vinclozolin (Ronilan) and chlozolinate (Serinal) (RF≅10). Therefore the resistance is partially crossed even if a high level of resistance was rarely observed for all dicarboximides (phenotype R2). At least two different mechanisms of resistance seem to be involved: one that may provide a moderate resistance and the other that may give a high resistance level. Monospore isolate sensitivity tests confirmed the qualitative response suggested by such high resistance factors.

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References

  • RE Beever HMR Brien (1983) ArticleTitleA survey of resistance to dicarboximide fungicides in Botrytis cinerea New Zealand Journal of Agricultural Research 26 391–400 Occurrence Handle1:CAS:528:DyaL2cXks1yqtA%3D%3D

    CAS  Google Scholar 

  • D Blancard E Allard P Brest (1989) ArticleTitleLa Stemphyliose du poirier ou « macules brunes » Phytoma 406 37–39

    Google Scholar 

  • A Brunelli I Gherardi N Adani (1997) ArticleTitleReduced sensitivity of Stemphylium vesicarium, causal agent of pear brown spot, to dicarboximide fungicides Informatore Fitopatologico 47 44–48

    Google Scholar 

  • M Collina I Gherardi A Brunelli (2002) ArticleTitleAcquired resistance of Stemphylium vesicarium to procymidone on pear in Italy Acta Horticulturae 596 547–549 Occurrence Handle1:CAS:528:DC%2BD3sXisFarsrg%3D

    CAS  Google Scholar 

  • RP Davis C Dennis (1981a) ArticleTitleProperties of dicarboximide-resistant strains of Botrytis cinerea Pesticide Science 12 521–535 Occurrence Handle10.1002/ps.2780120509

    Article  Google Scholar 

  • RP Davis C Dennis (1981b) ArticleTitleStudies on the survival and infective ability of dicarboximide-resistant strains of Botrytis cinerea Annals of Applied Biology 98 395–402 Occurrence Handle10.1111/j.1744-7348.1981.tb00771.x

    Article  Google Scholar 

  • IB Dry KH Yuan DG Hutton (2004) ArticleTitleDicarboximide resistance in field isolates of Alternaria alternata is mediated by a mutation in a two-component histidine kinase gene Fungal Genetics and Biology 41 IssueID1 102–108 Occurrence Handle10.1016/j.fgb.2003.09.002 Occurrence Handle14643263 Occurrence Handle1:CAS:528:DC%2BD3sXptF2qt70%3D

    Article  PubMed  CAS  Google Scholar 

  • PAG Elmer RE Gaunt (1993) ArticleTitleEffect of frequency of dicarboximide applications on resistant populations of Monilinia fructicola and brown rot in New Zealand orchards Crop Protection 12 83–88 Occurrence Handle10.1016/0261-2194(93)90132-3 Occurrence Handle1:CAS:528:DyaK3sXitVOnsL8%3D

    Article  CAS  Google Scholar 

  • PAG Elmer RE Gaunt (1994) ArticleTitleThe biological characteristics of dicarboximide-resistant isolates of Monilinia fructicola from New Zealand stone-fruit orchards Plant Pathology 43 130–137 Occurrence Handle10.1111/j.1365-3059.1994.tb00562.x

    Article  Google Scholar 

  • F Faretra E Antonacci S Serra L Cugudda (1986) ArticleTitlePreliminary observations on the spread of strains resistant to benzimidazoles and dicarboximides in Botrytis cinerea Pers. populations on grapevine in Apulia La difesa delle piante 9 13–22

    Google Scholar 

  • DF Farr GF Bills GP Chamuris AY Rossman ((1989)) Fungi on Plants and Plant Products in the United States APS Press St. Paul, Minnesota 1252

    Google Scholar 

  • SG Georgopoulos (1985) ArticleTitleThe genetic basis of classification of fungicides according to resistance risk EPPO Bulletin 15 513–517 Occurrence Handle10.1111/j.1365-2338.1985.tb00263.x

    Article  Google Scholar 

  • JM Gouot (1988) Characteristics and population dynamics of Botrytis cinerea and other pathogens resistant to dicarboximides CJ Delp BR Delp TM Fortii HV Morton CM Smith (Eds) Fungicide Resistance in North America. IV Dicarboximide Fungicides APS Press St. Paul, Minnesota 53–55

    Google Scholar 

  • ML Gullino MA Waard ParticleDe (1984) ArticleTitleLaboratory resistance to dicarboximides and ergosterol biosynthesis inhibitors in Penicillium expansum Netherlands Journal of Plant Pathology 90 177–179 Occurrence Handle10.1007/BF02006483 Occurrence Handle1:CAS:528:DyaL2MXlslKmsA%3D%3D

    Article  CAS  Google Scholar 

  • Y Hisada H Takaki Y Kawase T Ozaki (1979) ArticleTitleDifference in the potential of Botrytis cinerea to develop resistance to procymidone in vitro and in field Annals of the Phytopathological Society of Japan 45 283–290

    Google Scholar 

  • B Iacomi-Vasilescu H Avenot N Bataillé-Simoneau E Laurent M Guénard P Simoneau (2004) ArticleTitleIn vitro fungicide sensitivity of Alternaria species pathogenic to crucifers and identification of Alternaria brassicicola field isolates highly resistant to both dicarboximides and phenylpyrroles Crop Protection 23 481–488 Occurrence Handle10.1016/j.cropro.2003.10.003 Occurrence Handle1:CAS:528:DC%2BD2cXjtVCgt7s%3D

    Article  CAS  Google Scholar 

  • T Katan (1982) ArticleTitleResistance to 3,5-dichlorophenyl-N-cyclic imide (dicarboximide) fungicides in the grey mould pathogen Botrytis cinerea on protected crops Plant Pathology 31 133–141 Occurrence Handle1:CAS:528:DyaL38XlvFCnt7s%3D Occurrence Handle10.1111/j.1365-3059.1982.tb02821.x

    Article  CAS  Google Scholar 

  • CM Leach (1967) ArticleTitleInteraction of near-ultraviolet light and temperature on sporulation of the fungi Alternaria, Cercosporella, Fusarium, Helminthosporium and Stemphylium Canadian Journal of Botany 45 1999–2016

    Google Scholar 

  • P Leroux F Chapeland D Desbrosses M Gredt (1999) ArticleTitlePatterns of cross-resistance to fungicides in Botryotinia fuckeliana (Botrytis cinerea) isolates from French vineyards Crop Protection 18 687–697 Occurrence Handle10.1016/S0261-2194(99)00074-5 Occurrence Handle1:CAS:528:DC%2BD3cXhtVCjtrs%3D

    Article  CAS  Google Scholar 

  • P Leroux M Clerjeau (1985) ArticleTitleResistance of Botrytis cinerea Pers. and Plasmopara viticola (Berk. & Curt.) Berl. and de Toni to fungicides in French vineyards Crop Protection 4 137–160 Occurrence Handle10.1016/0261-2194(85)90014-6 Occurrence Handle1:CAS:528:DyaL2MXlvFWgsbo%3D

    Article  CAS  Google Scholar 

  • P Leroux R Fritz (1984) Antifungal activity of dicarboximides and aromatic hydrocarbons and resistance to these fungicides APJ Trinci JF Ryley (Eds) Mode of Action of Antifungal Agents Cambridge University Press Cambridge 207–237

    Google Scholar 

  • P Leroux R Fritz D Debieu C Albertini C Lanen J Bach M Gredt F Chapeland (2002) ArticleTitleMechanisms of resistance to fungicides in field strains of Botrytis cinerea Pest Management Science 58 876–888 Occurrence Handle10.1002/ps.566 Occurrence Handle12233177 Occurrence Handle1:CAS:528:DC%2BD38XmvFGjs74%3D

    Article  PubMed  CAS  Google Scholar 

  • G Lorenz (1988) Dicarboximide fungicides: History of resistance development and monitoring methods CJ Delp BR Delp TM Fortii HV Morton CM Smith (Eds) Fungicide Resistance in North America. IV Dicarboximide Fungicides APS Press St. Paul, Minnesota 45–51

    Google Scholar 

  • M Oshima M Fujimura S Banno C Hashimoto T Motoyama A Ichiishi I Yamaguchi (2002) ArticleTitleA point mutation in the two-component histidina kinase BcOS-1 gene confers dicarboximide resistance in field isolates of Botrytis cinerea Phytopathology 92 75–80 Occurrence Handle1:CAS:528:DC%2BD38Xlt1yqsA%3D%3D Occurrence Handle10.1094/PHYTO.2002.92.1.75 Occurrence Handle18944142

    Article  CAS  PubMed  Google Scholar 

  • CA Pappas DJ Fisher (1979) ArticleTitleA comparison of the mechanisms of action of vinclozolin, procymidone, iprodione and prochloraz against Botrytis cinerea Pesticide Science 10 239–246 Occurrence Handle1:CAS:528:DyaL3cXhs12ktA%3D%3D Occurrence Handle10.1002/ps.2780100308

    Article  CAS  Google Scholar 

  • EH Pommer G Lorenz (1982) ArticleTitleResistance of Botrytis cinerea Pers. to dicarboximide fungicides – a literature review Crop Protection 1 221–230 Occurrence Handle10.1016/0261-2194(82)90044-8 Occurrence Handle1:CAS:528:DyaL3sXhvFehsLg%3D

    Article  CAS  Google Scholar 

  • Ponti I, Brunelli A, Tosi C, Cavallini G and Mazzini F (1996) Chemical control trials on pear brown spot (Stemphylium vesicarium). In: Atti Giornate Fitopatologiche 1996 vol. 2 (pp.165–172)

  • I Ponti P Cavanni A Brunelli (1982) ArticleTitleBrown spot of pear: Aetiology and protection Informatore Fitopatologico 32 35–40

    Google Scholar 

  • DF Ritchie (1983) ArticleTitleMycelial growth, peach fruit-rotting capability and sporulation of strains of Monilinia fructicola resistant to dichloran, iprodione, procymidone and vinclozolin Phytopathology 73 44–47 Occurrence Handle10.1094/Phyto-73-44

    Article  Google Scholar 

  • ML Romano ML Gullino A Garibaldi (1983) ArticleTitleTests of competition in Petri dishes and on grapes in the laboratory between Botrytis cinerea Pers. strains sensitive and resistant to dicarboximides La difesa delle piante 2 75–80

    Google Scholar 

  • DA Rosenberger FW Meyer (1981) ArticleTitlePostharvest fungicides for apples: Development of resistance to benomyl, vinclozolin and iprodione Plant Disease 65 1010–1013 Occurrence Handle10.1094/PD-65-1010

    Article  Google Scholar 

  • EG Simmons (1969) ArticleTitlePerfect states of Stemphylium Mycologia 61 1–26 Occurrence Handle10.2307/3757341

    Article  Google Scholar 

  • Sisler HD (1988) Dicarboximide fungicides: Mechanisms of action and resistance. In: Delp CJ, Delp BR, Fortii TM, Morton HV and Smith CM (eds) Fungicide Resistance in North America. IV Dicarboximide Fungicides, (p. 52) APS Press

  • G Skylakakis (1985) ArticleTitleTwo different processes for the selection of fungicide-resistant sub-populations EPPO Bulletin 15 519–525 Occurrence Handle10.1111/j.1365-2338.1985.tb00264.x

    Article  Google Scholar 

  • CC Steel NG Nair (1993) ArticleTitleThe physiological basis of resistance to the dicarboximide fungicide iprodione in Botrytis cinerea Pesticide Biochemistry and Physiology 47 60–68 Occurrence Handle10.1006/pest.1993.1063 Occurrence Handle1:CAS:528:DyaK3sXms1KhtbY%3D

    Article  CAS  Google Scholar 

  • P Vilardell (1988) ArticleTitleStemphylium vesicarium en plantaciones de peral Fruticultura Profesional 18 51–55

    Google Scholar 

Download references

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Authors and Affiliations

  1. Dipartimento di Protezione e Valorizzazione Agroalimentare, Università di Bologna, Viale G. Fanin 46, 40127, Bologna, Italy

    Giulia Alberoni, Marina Collina, Davide Pancaldi & Agostino Brunelli

Authors
  1. Giulia Alberoni
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  2. Marina Collina
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  3. Davide Pancaldi
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  4. Agostino Brunelli
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Correspondence to Marina Collina.

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Alberoni, G., Collina, M., Pancaldi, D. et al. Resistance to Dicarboximide Fungicides in Stemphylium vesicarium of Italian Pear Orchards. Eur J Plant Pathol 113, 211–219 (2005). https://doi.org/10.1007/s10658-005-2332-3

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