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Evaluation of biocontrol preparations and plant extracts for the control of Phytophthora infestans on potato leaves

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Abstract

The potential of biocontrol products and plant extracts for control of late blight on potato plants, caused by Phytophthora infestans was evaluated in detached leaf assays and on potted plants. Based on an initial screening of 22 preparations and plant extracts, the 10 most active treatments were selected for further investigation. In the detached leaf assays the commercial preparations Elot-Vis, Serenade and Trichodex, and plant extracts of Rheum rhabarbarum and Solidago canadensis showed a significant effect on the level of infestation by P. infestans. However, none of the treatments was as effective as copper. In the case of Serenade, the metabolites produced by its active micro-organism, Bacillus subtilis, were demonstrated to be the effective component of the formulation, and not the micro-organism itself. In order to take curative and protective modes of action into account, the test substances were applied 24 h before, or 90 min after inoculation with P. infestans. Generally, better effects were obtained when the applications were made 24 h before inoculation. For defining the optimum time of application, potted plants were treated 72 or 24 h before, and 1 and 24 h after inoculation with P. infestans. In these tests, Trichodex showed no activity, while Elot-Vis gave best results when applied 1 day before inoculation. Serenade and the extracts of R. rhabarbarum and S. canadensis (all at 5% concentration) however, were effective when applied up to 3 days before and just after inoculation with P. infestans. The results of the experiments on potted plants indicated direct effects on the pathogen for all agents except the extract of S. canadensis, but other mode of actions, e.g. induced resistance, could not be ruled out. None of the treatments had a curative effect.

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Authors and Affiliations

  1. Federal Biological Research Centre for Agriculture and Forestry, Institute for Biological Control, Heinrichstr. 243, D-64287, Darmstadt, Germany

    D. Stephan, A. Schmitt & E. Koch

  2. Department of Agriculture & Forestry, School of Biological Sciences, University of Aberdeen, Hilton Campus, Hilton Place, AB24 4FA, Aberdeen, UK

    S. Martins Carvalho & B. Seddon

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  1. D. Stephan
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  2. A. Schmitt
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  3. S. Martins Carvalho
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  4. B. Seddon
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  5. E. Koch
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Correspondence to D. Stephan.

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Stephan, D., Schmitt, A., Carvalho, S.M. et al. Evaluation of biocontrol preparations and plant extracts for the control of Phytophthora infestans on potato leaves. Eur J Plant Pathol 112, 235–246 (2005). https://doi.org/10.1007/s10658-005-2083-1

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  • DOI: https://doi.org/10.1007/s10658-005-2083-1

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