Skip to main content

Pseudomonas aeruginosa inhibits the plant cell wall degrading enzymes of Sclerotium rolfsii and reduces the severity of groundnut stem rot

Abstract

Three hundred and ninety-three groundnut-associated bacterial strains, applied both as seed treatment and soil amendment, were evaluated for control of stem rot disease (caused by Sclerotium rolfsii) of groundnut in a controlled environment. Twelve strains significantly (P=0.01) reduced the incidence of stem, rot of which groundnut seed endophytes Pseudomonas aeruginosa GSE 18 and GSE 19 reduced the seedling mortality by 54% and 58%, compared to the control. In dual cultures, the 12 biocontrol strains reduced the mycelial growth of S. rolfsii by 32%–74% as compared to the control. Cell- free culture filtrates of P. aeruginosa GSE 18 and GSE 19 inhibited the activity in vitro of the cell wall-degrading enzymes (CWDE) polygalacturonase and cellulase by S. rolfsii up to a maximum of 55% and 50%, respectively, when measured 6 days after inoculation. Pseudomonas aeruginosa GSE 18 and GSE 19 with a known tolerance to thiram, a commonly used seed dressing fungicide, suppressed the growth of S. rolfsii, inhibited the activity of CWDE, and reduced the incidence of stem rot, suggesting the usefulness of these biocontrol strains as components in the integrated management of groundnut stem rot.

This is a preview of subscription content, access via your institution.

References

  1. DF Bateman SV Beer (1965) ArticleTitleSimultaneous production and synergistic action of oxalic acid and polygalacturonase during pathogenesis by Sclerotium rolfsii Phytopathology 55 204–211 Occurrence Handle14274523 Occurrence Handle1:STN:280:DyaF2M%2FnvFSrtQ%3D%3D

    PubMed  CAS  Google Scholar 

  2. BL Bertagnolli FKD Soglio JB Sinclair (1996) ArticleTitleExtracellular enzyme profiles of the fungal pathogen Rhizoctonia solani isolate 2B−12 and of two antagonists, Bacillus megaterium strain B153-2-2 and Trichoderma harzianum isolate Th008. I. Possible correlations with inhibition of growth and biocontrol Physiological and Molecular Plant Pathology 48 145–160 Occurrence Handle1:CAS:528:DyaK28Xis1Olurs%3D Occurrence Handle10.1006/pmpp.1996.0013

    CAS  Article  Google Scholar 

  3. C Campion P Massiot F Rouxel (1997) ArticleTitleAggressiveness and production of cell wall-degrading enzymes by Pythium violae, Pythium sulcatum and Pythium ultimum, responsible for cavity spot on carrots European Journal of Plant Pathology 103 725–735 Occurrence Handle1:CAS:528:DyaK1cXlsl2rtg%3D%3D Occurrence Handle10.1023/A:1008657319518

    CAS  Article  Google Scholar 

  4. A Collmer JL Reid MS Mount (1988) ArticleTitleAssay methods for pectic enzymes Methods in Enzymology 161 329–335 Occurrence Handle1:CAS:528:DyaL1MXhsFShsLc%3D Occurrence Handle10.1016/0076-6879(88)61037-8

    CAS  Article  Google Scholar 

  5. RM Cooper (1983) The mechanisms and significance of enzymatic degradation of host cell walls by parasites JA Callow (Eds) Biochemical Plant Pathology John Wiley New Jersey 101–135

    Google Scholar 

  6. Y Elad A Kapat (1999) ArticleTitleThe role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea European Journal of Plant Pathology 105 177–189 Occurrence Handle1:CAS:528:DyaK1MXjvVCnur4%3D Occurrence Handle10.1023/A:1008753629207

    CAS  Article  Google Scholar 

  7. DR Fravel (1988) ArticleTitleRole of antibiosis in the biocontrol of plant diseases Annual Review of Phytopathology 26 75–91 Occurrence Handle1:CAS:528:DyaL1cXlvVGgurg%3D

    CAS  Google Scholar 

  8. P Ganesan SS Gnanamanickam (1987) ArticleTitleBiological control of Sclerotium rolfsii in peanut by inoculation with Pseudomonas fluorescens Soil Biology and Biochemistry 19 35–38 Occurrence Handle10.1016/0038-0717(87)90122-2

    Article  Google Scholar 

  9. A Kapat G Zmand Y Elad (1998) ArticleTitleEffect of two isolates of Trichoderma harzianum on the activity of hydrolytic enzymes produced by Botrytis cinerea Physiological and Molecular Plant Pathology 52 127–137 Occurrence Handle1:CAS:528:DyaK1cXktFahs7Y%3D Occurrence Handle10.1006/pmpp.1997.0140

    CAS  Article  Google Scholar 

  10. GK Kishore S Pande AR Podile (2005a) ArticleTitlePhylloplane bacteria increase seedling emergence, growth and yield of field-grown groundnut (Arachis hypogaea L.) Letters in Applied Microbiology 40 260–268 Occurrence Handle1:CAS:528:DC%2BD28XhvFGhsbw%3D Occurrence Handle10.1111/j.1472-765X.2005.01664.x

    CAS  Article  Google Scholar 

  11. GK Kishore S Pande AR Podile (2005b) ArticleTitleBiological control of collar rot disease with broad-spectrum antifungal bacteria associated with groundnut Canadian Journal of Microbiology 51 123–132 Occurrence Handle1:CAS:528:DC%2BD2MXksFKitbc%3D Occurrence Handle10.1139/w04-119

    CAS  Article  Google Scholar 

  12. K Manjula GK Kishore AG Girish SD Singh (2004) ArticleTitleCombined application of Pseudomonas fluorescens and Trichoderma viride has an improved biocontrol activity against stem rot in groundnut The Plant Pathology Journal 20 75–80

    Google Scholar 

  13. VK Mehan CD Mayee D McDonald (1994) ArticleTitleManagement of Sclerotium rolfsii-caused stem and pod rots of groundnut␣– a critical reivew International Journal of Pest Management 40 313–320 Occurrence Handle10.1080/09670879409371906

    Article  Google Scholar 

  14. GL Miller (1959) ArticleTitleUse of dinitrosalicylic acid reagent for determination of reducing sugar Analytical Chemistry 31 426–428 Occurrence Handle1:CAS:528:DyaG1MXmtFKiuw%3D%3D

    CAS  Google Scholar 

  15. DJ O’Sullivan F O’Gara (1992) ArticleTitleTraits of fluorescent Pseudomonas spp involved in suppression of plant root pathogens Microbiological Reviews 56 662–676 Occurrence Handle1480114 Occurrence Handle1:STN:280:ByyC38rksVQ%3D

    PubMed  CAS  Google Scholar 

  16. AR Podile GK Kishore (2002) Biological control of peanut diseases SS Gnanamanickam (Eds) Biological Control of Major Crop Plant Diseases Marcel & Dekker New York 131–160

    Google Scholar 

  17. JM Raaijmakers M Vlarni JT deSouza (2002) ArticleTitleAntibiotic production by bacterial biocontrol agents Antonie van Leeuwenhoek 81 537–547 Occurrence Handle12448749 Occurrence Handle1:CAS:528:DC%2BD38Xns1SgsbY%3D Occurrence Handle10.1023/A:1020501420831

    PubMed  CAS  Article  Google Scholar 

  18. A Sessitch B Reiter G Berg (2004) ArticleTitleEndophytic bacterial communities of field-grown potato plants and their plant-growth-promoting and antagonistic abilities Canadian Journal of Microbiology 50 239–249

    Google Scholar 

  19. FM Shokes K Rhogalski DW Gorbet TB Brenneman DA Berger (1996) ArticleTitleTechniques for inoculation of peanut with Sclerotium rolfsii in the greenhouse and field Peanut Science 23 124–128 Occurrence Handle10.3146/i0095-3679-23-2-11

    Article  Google Scholar 

  20. UP Singh BK Sarma DP Singh (2003) ArticleTitleEffect of plant growth-promoting rhizobacteria and culture filtrate of Sclerotium rolfsii on phenolic and salicylic acid contents in chickpea (Cicer arietinum) Current Microbiology 46 131–140 Occurrence Handle12520369 Occurrence Handle1:CAS:528:DC%2BD3sXhtlOhs7c%3D Occurrence Handle10.1007/s00284-002-3834-2

    PubMed  CAS  Article  Google Scholar 

  21. DM Weller (1988) ArticleTitleBiological control of soilborne plant pathogens in the rhizosphere with bacteria Annual Review of Phytopathology 26 379–407 Occurrence Handle10.1146/annurev.py.26.090188.002115

    Article  Google Scholar 

  22. JM Whipps (2001) ArticleTitleMicrobial interactions and biocontrol in the rhizosphere Journal of Experimental Botany 52 487–511 Occurrence Handle11326055 Occurrence Handle1:CAS:528:DC%2BD3MXjs1ajsrk%3D

    PubMed  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to A. R. Podile.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kishore, G.K., Pande, S., Rao, J.N. et al. Pseudomonas aeruginosa inhibits the plant cell wall degrading enzymes of Sclerotium rolfsii and reduces the severity of groundnut stem rot. Eur J Plant Pathol 113, 315–320 (2005). https://doi.org/10.1007/s10658-005-0295-z

Download citation

Keywords

  • antifungal bacteria
  • Arachis hypogaea
  • cellulase
  • necrotrophic fungi
  • peanut
  • polygalacturonase