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Adherence to a plant-based, high-fibre dietary pattern is related to regression of non-alcoholic fatty liver disease in an elderly population

European Journal of Epidemiology volume 35, pages 1069–1085 (2020)Cite this article

Abstract

Dietary lifestyle intervention is key in treating non-alcoholic fatty liver disease (NAFLD). We aimed to examine the longitudinal relation between well-established dietary patterns as well as population-specific dietary patterns and NAFLD. Participants from two subsequent visits of the Rotterdam Study were included. All underwent serial abdominal ultrasonography (median follow-up: 4.4 years) and filled in a food frequency questionnaire. Secondary causes of steatosis were excluded. Dietary data from 389 items were collapsed into 28 food groups and a posteriori dietary patterns were identified using factor analysis. Additionally, we scored three a priori dietary patterns (Mediterranean Diet Score, Dutch Dietary Guidelines and WHO-score). Logistic mixed regression models were used to examine the relation between dietary patterns and NAFLD. Analyses were adjusted for demographic, lifestyle and metabolic factors. We included 963 participants of whom 343 had NAFLD. Follow-up data was available in 737 participants. Incident NAFLD was 5% and regressed NAFLD was 30%. We identified five a posteriori dietary patterns (cumulative explained variation [R2] = 20%). The patterns were characterised as: vegetable and fish, red meat and alcohol, traditional, salty snacks and sauces, high fat dairy & refined grains pattern. Adherence to the traditional pattern (i.e. high intake of vegetable oils/stanols, margarines/butters, potatoes, whole grains and sweets/desserts) was associated with regression of NAFLD per SD increase in Z-score (0.40, 95% CI 0.15–1.00). Adherence to the three a priori patterns all showed regression of NAFLD, but only the WHO-score showed a distinct association (0.73, 95% CI 0.53–1.00). Hence, in this large elderly population, adherence to a plant-based, high-fibre and low-fat diet was related to regression of NAFLD.

Introduction

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide and characterized by fat accumulation in the liver without the presence of the well-known risk factors for liver disease such as alcohol misuse and viral hepatitis [1]. The increase of NAFLD parallels the worldwide rise of non-communicable metabolic diseases, such as obesity and type 2 diabetes mellitus [2, 3]. In fact, it is predicted that NAFLD prevalence will continue to increase, which will subsequently lead to even more advanced liver disease and liver-related mortality in the coming years [4]. But apart from the liver-related sequelae, NAFLD is also regarded as the hepatic manifestation of the metabolic syndrome [5]. It is not only strongly associated with metabolic health, but it also actively contributes to the risk of cardiovascular disease incidence [6, 7].

Pathogenesis of NAFLD is multifactorial—there are many molecular pathways that contribute to the development of NAFLD—and it is likely that NAFLD pathogenesis differs between individuals [1]. A common denominator in the development of NAFLD, however, is diet. Furthermore, as there is no registered drug for the treatment of NAFLD, the mainstay of treatment is implementing a healthy diet and stimulating physical activity [8]. It has been repeatedly shown that weight loss of 5% or more of the total body weight is beneficial for liver health [9,10,11], meanwhile the Mediterranean diet has been shown to reduce liver fat independent of weight loss [12, 13]. To date, the Mediterranean dietary pattern is indeed regarded as the diet of choice for NAFLD [12]. And although there is a paramount of studies on the association between separate food items or groups with NAFLD, evidence on diet as a whole almost exclusively originates from either small or cross-sectional studies [12, 14,15,16]. Studying pre-hypothesized individual food items or nutrients can be of value, nonetheless, this approach has some important drawbacks [17]. For instance, one does not eat isolated nutrients but instead a complex mix of foods which can interact with each other, and moreover, foods are highly inter-correlated with each other. In addition, the effect of one nutrient can be small. Therefore, studying their separate effects is challenging in a real-life setting. Conceptually, the study of dietary quality—by means of dietary patterns—represents more information on dietary habits, food and nutrient consumption [18]. Recently, Ma et al. [19] showed for the first time, that high dietary quality, as assessed by the a priori hypothesized Mediterranean Diet Score (MDS) and the American Heart Eating Index, was associated with reduced odds of steatosis development in a large longitudinal cohort study.

Another way to study dietary patterns is the use of factor analysis to derive population-specific patterns, so-called a posteriori dietary patterns. To date, a few small cross-sectional studies appraised the association between a posteriori dietary patterns and steatosis. All identified an unhealthy dietary pattern that was associated with steatosis [16, 20, 21].

The combination of both a priori and a posteriori patterns might reveal a better understanding of the relation between dietary quality and NAFLD. The objective of this study was, therefore, to examine the relation between dietary patterns and NAFLD prospectively in an elderly predominantly Caucasian population. Specifically, we studied the following a priori dietary patterns: (1) the Mediterranean Diet Score, (2) the Dutch Dietary Guidelines, and (3) the World Health Organization recommendations. In addition, we identified five population-specific a posteriori patterns.

Methods

Study cohort

This study is embedded in the large ongoing population-based cohort study entitled the Rotterdam Study (RS). The RS commenced in 1989 and was designed to study elderly diseases as a response to the increasing proportion of elderly people in the population. A detailed description of the design and rationale has been described previously [22]. For the purpose of this study, we used two subsequent visits from the second cohort (RSII-3 and RSII-4). All individuals in this cohort were included based on their ZIP-code (being the suburb Ommoord in Rotterdam) and their age (at first inclusion 55 years or older).

We excluded participants that did not undergo ultrasound and participants with missing or unreliable food frequency questionnaires (FFQ), i.e. caloric consumption below 500 or above 7500 kilocalories (kcal) per day. We excluded unreliable transient elastography (TE), i.e. an interquartile range (IQR)/median liver stiffness measurement (LSM) > 0.30 in measurements with a median of ≥ 7.1 kilopascals (kPa) or above [23] and failure of TE (i.e. if no LSM was measured after at least ten attempts). Lastly, we excluded participants with well-known risk factors for steatosis, such as the presence of viral hepatitis (as measured by HbsAg and anti-HCV), the presence of alcohol misuse (measured using FFQs and defined as ≥ 2 units of alcohol per day in women and ≥ 3 units in men), or the use of pharmacy-registered drugs that are known to cause steatosis (i.e. tamoxifen, methotrexate, systemic corticosteroids, and amiodarone).

The RS has been approved by the institutional review board (Medical Ethics Committee) of the Erasmus MC University Medical Centre Rotterdam and by the review board of The Netherlands Ministry of Health, Welfare and Sports. Written informed consent was obtained from all participants.

Food frequency questionnaires

Participants were asked to fill in a semi-quantitative 389-item FFQ, specifically developed for Dutch adults, during both research visits (RSII-3 and RSII-4) [24, 25]. This FFQ includes detailed questions on consumption over the last month and deals with type of food item, portion size, preparation method and frequency of consumption [26]. Questions within the FFQ are for example: “Did you eat eggs last month? If yes, how were they prepared (boiled or baked)? How often did you eat eggs per month (once or 2–3 times) or per week (once, 2–3, 4–5, 6–7 times)? How many eggs did you eat on an average day then?”. The 389 food items were grouped into 28 empirical food groups (supplementary Table 1), based on previous publications [20, 21, 27], and adapted based on the food item and group quantities (i.e. merged similar groups with a very low median intake).

A priori dietary patterns

We chose to study the Mediterranean Diet Score (MDS), the Dutch Dietary Guidelines (DDG), and the World Health Organization (WHO) recommendations as a priori dietary patterns.

The MDS, first described by Trichopoulou et al. [28], originally has 9 components, of which 7 components are regarded beneficial (i.e. vegetables, legumes, fruits, nuts, whole grains, fish and mono-unsaturated fatty acids (MUFA)-to-saturated fatty acids ratio) and 2 components are regarded hazardous (i.e. red meat intake and excessive alcohol use). For the purpose of this study, we adapted the MDS by excluding the alcohol component from our calculation, as the MDS cut-off for hazardous alcohol use is 50 g per day, which is a very high cut-off in the context of a hepatic health outcome [29]. Moreover, alcohol consumption was included in the multivariable model as potential confounder. Hence, our adapted MDS has 8 components. All components were given a score of 0 (unhealthy) or 1 (healthy) based on sex specific median cut-offs, and summed up.

The DDG is a predefined index that was developed in 2015 and describes a general advice to follow a balanced and healthy dietary pattern [30]. The DDG is scored on the following points, consumption of (1) vegetables (≥ 200 g/day), (2) fruit (≥ 200 g/day), (3) whole-grain products (≥ 90 g/day), (4) legumes (≥ 135 g/week), (5) unsalted nuts (≥ 15 g/day), (6) fish (≥ 100 g/week), (7) dairy (≥ 350 g/day), (8) tea (≥ 150 mL/day), (9) whole grains ≥ 50% of total grains, (10) unsaturated fats and oils ≥ 50% of total fats, (11) red and processed meat < 300 g/week, (12) sugar-containing beverages (≤ 150 mL/day), (13) alcohol (≤ 10 g/day), and (14) salt (≤ 6 g/day).

We calculated the WHO-score based on the recent revised guidelines of the WHO (October 2018) [31]. This score is composed of 6 components which are scored as 0 if unhealthy and 1 if healthy. The components are scored as healthy if they satisfied the following criteria: (1) vegetables and fruit intake of ≥ 400 g per day, (2) sugar intake (added and sugar sweetened beverages) of < 10 g per day, (3) energy percentage from fat intake < 30%, (4) energy percentage from saturated fat intake < 10%, (5) energy percentage from trans fatty acid intake is < 1%, and (6) salt intake of < 5 g per day.

A posteriori dietary patterns

A priori dietary patterns signify patterns described/identified in previous studies or specific habits of certain populations. They therefore do not necessarily ‘fit’ every population. For example, the Mediterranean Diet is natural for the Greek population in which this diet has been developed, whereas other populations such as the Dutch or Asian have different eating habits. We believe it is therefore of interest to also use a posteriori dietary patterns. These are population-specific dietary patterns and were derived using factor analysis on the 28 food groups at baseline with Varimax rotation and minimum residual estimation, using the function “fa” from the R package psych [32]. We included 5 dietary patterns based on the bend in the scree plot (supplementary Fig. 1). The factor loadings for each food group reflect the relationship between the food group and the respective factor (i.e. dietary pattern). Subsequently we calculated adherence scores, separately for both visits, by multiplying the factors (determined for the food groups at baseline) with the observed values of the food groups at baseline (RSII-3) and follow-up (RSII-4), respectively. Each score at baseline was scaled to have a mean zero and a standard deviation (SD) of one. The same scaling parameters were used for the corresponding score at follow-up, to optimize comparability.

Hepatic imaging

For the purpose of this study, all participants underwent an abdominal ultrasound (Hitachi HI VISION 900) and TE (FibroScan®, EchoSens, Paris, France). Both examinations were performed at the same visit by an experienced nurse technician. The diagnosis of steatosis was dichotomized as yes or no, because of the poor sensitivity for the grading of steatosis but the good performance for diagnosing moderate/severe steatosis [33]. Steatosis was defined as hyperechogenic liver parenchyma as compared to the kidney parenchyma [34]. The practical performance of the transient elastography has been described in detail previously [35]. In short, both M and XL probe were available for the liver stiffness measurements (LSM) and used dependent on the subcutaneous fat layer as instructed by the manufacturer. Reliability criteria are described in the paragraph above (“Study Cohort”). Additionally, participants with an intra-cardiac device were excluded from the analyses. LSM were given as kilopascals (kPa). We used the previously proposed cut-off value of 8 kPa to proxy the presence of fibrosis in participants with steatosis, from this point forward referred to as non-alcoholic steatofibrosis (NASF) [36]. As the main focus of this present study is NAFLD, participants with an LSM of 8 kPa or higher without steatosis were excluded.

Other covariates

All blood samples were drawn after overnight fasting. Automatic enzyme procedures (Roche Diagnostics GmbH, Mannheim, DE) were used to determine lipid profile, glucose, alanine aminotransferase, aspartate aminotransferase and gamma-glutamyltransferase. Insulin and viral hepatitis B or C were determined using an automatic immunoassay (Roche Diagnostics GmbH, Mannheim, DE). Detailed information on drug use was obtained via automated pharmacy linkage (with which 98% of the participants were registered). A 3 h home interview was carried out by trained research nurses and included questions on physical activity, smoking behaviour, education level, medical history, and demographics. In the research centre, anthropometrics were measured (i.e. weight, height, and waist and hip circumference), as well as blood pressure (median value after two measures in an upright position). The metabolic syndrome was defined using the harmonizing consensus criteria from Alberti et al. and contained 5 components on abdominal obesity, lipid profile, blood pressure, and fasting plasma glucose [37]. The comorbidities diabetes mellitus and hypertension were established on the basis of drug use for the respective comorbidity or findings at physical examination, as described in detail previously [38].

Statistical analyses

Participant’s characteristics at baseline and follow-up are summarized using the median and first and third quartile, median and range (for dietary variables), or percentages.

To examine the association between the dietary patterns, micronutrient and macronutrient composition, we calculated and tested Spearman correlation coefficients between the raw values of (subtypes of) macronutrients as well as adjusted for total energy intake and the components of the a posteriori dietary patterns, and the adherence scores at baseline. Differences between energy-adjusted and energy & dietary pattern-adjusted correlation coefficients may be explained by overlapping characteristics of the different a posteriori patterns, which could outweigh each other’s effects.

In addition, as a supplementary analysis, we assessed the cross-sectional association between the different energy-adjusted food groups and NAFLD at baseline using univariable logistic regression. The energy-adjustment was carried out using the residual method [39].

To investigate the association of diet with NAFLD over time in the presence of missing values in the covariates we used Bayesian logistic mixed models, as implemented in the R package JointAI [40]. In this approach missing values in covariates are imputed simultaneously with the estimation of the regression coefficients of interest, and the added uncertainty in the coefficients due to the missing values is automatically taken into account [41, 42]. This imputation was done using the covariates of our most extensive set (i.e. Model 2, given below). Please find more information on the missing data and imputed values in the separate supplementary data file. The choice of a mixed model allowed us to include data from all patients that fulfilled the above mentioned inclusion criteria, even when no follow-up measurement was available. A random intercept was included in the mixed model to take into account correlation between repeated measurements within the same subject. Separate models were fitted for each of the a priori dietary patterns and the five a posteriori patterns. As the five a posteriori dietary patterns explain approximately 20% of the variation in dietary intake in the population, they were analysed together in one model.

Two sets of covariates were created. The first set (“Model 1”) contains baseline age (in years), physical activity (in metabolic equivalent task h/week) and education level (low/intermediate/high), and in addition, sex, energy intake (in kilocalories per day), alcohol consumption (in units per day), and follow-up time (in years). The second set (“Model 2”) additionally contains covariates that reflect potential confounding, colliding, or mediating factors, i.e. baseline type 2 diabetes mellitus, baseline hypertension, and BMI. To allow the effect of diet to change over time and to allow effect modification by BMI interaction terms between the respective dietary pattern variable(s) and follow-up time (in Model 1 and 2) and BMI (only in Model 2) were included.

To obtain results for Model 1, ten sets of imputed values were extracted from each of the analyses of Model 2, then Model 1 was fitted on each dataset. Output from the ten repeated analyses per model was combined to calculate overall results. Since none of the interaction terms mentioned above had relevant contribution to any of the models, and the presence of interaction terms in a model complicates the interpretation of the regression coefficients substantially, we re-fitted Model 1 and Model 2 without the interaction terms (using imputed values from the original models) and present only the results of these simplified models.

We also investigated the role of BMI as a mediating factor between diet and NAFLD: we performed additional analyses with BMI (continuous) as outcome measure. For this, Bayesian linear mixed models were used and incomplete covariates were again simultaneously imputed. The models contained the confounders from Model 2, an interaction term between the dietary patterns and follow-up time, and a random intercept.

We also examined adherence to dietary patterns in relation to NAFLD severity. Due to the low number of NASF patients, we were not able to perform mixed effects logistic regression models on this outcome. In order to gain insight into the association between dietary patterns and NASF, we therefore plotted the (a posteriori and a priori) dietary pattern adherence scores across participants with NASF, participants with ‘simple’ steatosis, and participants without steatosis.

We used non-informative priors for our Bayesian analyses. Results from the Bayesian analyses are presented as posterior means and 95% credible intervals (CI). All analyses were performed using R version 3.5.2 and the packages JointAI (version 0.5.1) and psych (version 1.8.12). More detailed information of the statistical analyses can be found in the supplementary methods.

Results

Participant characteristics

The flowchart of the study is illustrated in Fig. 1. After exclusion, 963 (60.1%) participants were eligible for this study at baseline. Prevalence of men (n = 424/963 vs n = 280/639) and steatosis (n = 343/963 vs n = 252/639) were similar in the included and excluded group (P = 0.93 and P = 0.12 respectively). BMI was slightly lower in the included group (mean 27.2 ± 3.8 kg/m2) than in the excluded (mean 28.0 ± 4.4 kg/m2) group (P < 0.01) as was the mean age (72.0 ± 4.8 in the included group and 72.7 ± 5.7 in the excluded group; P < 0.01). Of all included participants, 343 had NAFLD (35.6%), of which 31 (9%) had coincident elevated LSM, i.e. NASF (Table 1).

Fig. 1
figure 1

Flowchart of the study. The dotted encircled ovals depict the diagnosis at follow up RS II-4 for the ‘no NAFLD’ and ‘NAFLD’ group in RS II-3. The numbers of ‘No NAFLD’ and ‘NAFLD’ in RS II-4 depicts these encircled numbers minus the exclusion of 55 unreliable or missing FFQ at follow-up. Please find supplementary Table 2 for more information. FFQ Food Frequency Questionnaire, FU follow-up, KPa Kilopascals, LSM liver stiffness measurements, NAFLD non-alcoholic fatty liver disease, RS Rotterdam Study, TE transient elastography, US ultrasound

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Table 1 Participant characteristics
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Follow-up data of 737 participants (76.5%) was available, measured at a median time of 4.4 years [4.3—4.5] after the baseline measurement. Of those lost to follow-up, no participant was registered as deceased. Most participants had a similar ultrasound diagnosis at baseline and follow-up (Fig. 1). However, in the group without steatosis, 24 participants progressed to steatosis (5.1%), and in the group with steatosis, 68 participants regressed to no steatosis (29.8%). In supplementary Table 2 a detailed overview of follow-up by liver status is given.

Participant characteristics at baseline and follow-up are given in Table 1. In short, at baseline, median age was 71 [69–73] years, 56% were female, median BMI was 26.9 [24.5—29.4] kg/m2, and median LSM was 4.9 [4.0—6.1] kPa. At follow-up, median age was 75 [73–77] years, 55% was female, median BMI was 26.5 [24.3—29.2] kg/m2, and median LSM was 5.1 [4.2—6.1] kPa.

Adherence scores to all dietary patterns at baseline and follow-up are given in Table 1. In general, adherence to healthy eating patterns was similar and low. Median energy intake, as well as adherence to dietary guidelines was comparable at baseline and follow-up. In this population, absolute intake was quite low, the most consumed food groups were coffee (406 g or 1.6 cups/day), fruit (301 g/day), and vegetables (211 g/day) (Table 2). The energy-adjusted associations between food groups and NAFLD at baseline are given in supplementary Fig. 2.

Table 2 Median absolute intake and factor loadings for food groups per a posteriori dietary pattern
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A posteriori dietary patterns

The a posteriori patterns obtained by factor analysis are presented in Table 2 and Fig. 2. We identified five dietary patterns which together explained 19.5% of the variation in food intake. Specifically, the patterns explained respectively 4.8%, 4.5%, 4.3%, 3.3% and 2.5% of the variation in food intake. The first pattern was characterised by high intake of vegetables, poultry, fish, and fruit. This pattern was therefore named vegetable and fish. The second pattern was characterised by high intake of red, refined or organ meat, salty snacks, and beer or spirits, and low intake of fruit and tea. We therefore called this pattern red meat and alcohol. The third pattern was defined by high intake of vegetable oils and stanols and margarines or butters, potatoes, whole grains, and sweet snacks or desserts. This pattern was therefore called traditional. The fourth pattern was represented by high intake of savoury food groups such as nuts, legumes, salty snacks, and sauces. We thus named this pattern salty snacks and sauces. The last pattern was defined by high intake of fruit juice, refined grains, high-fat dairy products, and sweet snacks or desserts. This pattern was therefore called high-fat dairy and refined grains.

Fig. 2
figure 2

Heatmap of factor loadings from a posteriori dietary patterns. Factor loadings of a posteriori patterns per food group visualized using a heatmap. Red reflects a positive load, blue a negative load

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Dietary pattern characteristics

In order to give more insight in the overall macronutrient and micronutrient composition of the dietary patterns, we performed Spearman rank correlation analyses at baseline (Fig. 3a–h).

Fig. 3
figure 3figure 3

Correlation coefficients of adherence scores with standardized unadjusted, energy adjusted and energy and dietary pattern adjusted macronutrients and micronutrients. These figures reflect the Spearman correlation coefficients of various micronutrients and macronutrients per dietary pattern. The orange bars reflect the unadjusted correlation coefficients, the yellow bars the correlation adjusted for energy intake using the residual method, and the third green bar (in the a posteriori dietary patterns) reflects the correlation adjusted for other a posteriori dietary patterns and energy intake. MUFA mono-unsaturated fatty acid, PUFA poly-unsaturated fatty acid

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The bars in orange reflect the unadjusted correlation coefficients and those in yellow the energy-adjusted coefficients. If the energy-adjusted correlation is lower than the unadjusted one, this indicates that, although the relative intake may be high, the absolute intake of the nutrient for that pattern is low. Which is, for example, the case in fat consumption for the DDG and the MDS (Fig. 3a, b). All a priori dietary patterns had a high energy-adjusted correlation with fibre and vegetable protein intake, and a negative correlation with overall fat intake (Fig. 3a–c). In addition, the DDG and the WHO-score had a relatively high correlation for mono-and disaccharide intake.

In Fig. 3d–h a third, green bar reflects the energy & dietary pattern-adjusted correlation. We added these results here since all patterns are analysed together in one regression model.

A posteriori patterns that resemble the healthy a priori dietary patterns most are the traditional and salty snacks and sauces patterns, which are also low in animal protein, fats, sodium and haem-iron, and high in vegetable protein, fibre, mono-and disaccharides and potassium (Fig. 3f, g). Whereas the red meat and alcohol and high-fat dairy and refined grains patterns were low in fibre and potassium and high in fats and haem iron (Fig. 3e, h).

Relation between dietary patterns and NAFLD

A summary of the multivariable logistic mixed models between the a priori dietary patterns and risk of NAFLD are given in Table 3. Results indicate regression of NAFLD in all patterns, but only with an unambiguous credible interval for the WHO-score after full adjustment for sociodemographic, lifestyle and metabolic confounders (Table 3; OR 0.73, 95% CI 0.35–1.00).

Table 3 Summary of parameters of interest from logistic mixed models for risk of NAFLD with adherence to a priori dietary patterns
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The multivariable associations between a posteriori dietary patterns and risk of NAFLD is shown in Table 4. Adherence to the traditional dietary pattern was associated with regression of NAFLD after full adjustment (Table 4; OR 0.40, 95% CI 0.15–1.00) and adherence to the salty snacks and sauces pattern showed a similar result (OR 0.43, 95% CI 0.17–1.04).

Table 4 Logistic mixed model for risk of NAFLD with adherence to a posteriori dietary patterns
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Factors that were independently associated incidence of NAFLD were incremental energy intake, BMI, baseline diabetes and baseline hypertension. Factors that were associated with regression of NAFLD were female gender, follow-up time, and high education level (Table 4 and supplementary Table 3a–c).

Additional analyses for BMI

In supplementary Table 4 we show the association between adherence to a posteriori and a priori dietary patterns with BMI over time. Taking interactions into account, BMI over time depended on the adherence of DDG and WHO-scores (i.e. low scores: BMI decreased, high scores: BMI increased). For MDS the interaction effect was so small that irrespective of the dietary score BMI generally increased over time. At baseline, none of the dietary patterns was associated with BMI, except for a priori pattern WHO which was associated with a lower BMI (supplementary Table 4d). In the model using a posteriori dietary patterns the effect of time on BMI depended on the score of the high fat dairy & refined grains pattern pattern. For low scores in this pattern the model estimated that BMI increased over time, whereas for high scores a slight decrease of BMI was found (supplementary Table 4a). On the other hand, there was a significant positive interaction between follow-up time and DDG and between follow-up time and WHO, which means the that BMI increased over time when adhering to these patterns and decreases for participants that had low scores on these patterns (supplementary Table 4c and d).

Visualization of dietary patterns for NAFLD severity

Adherence to the different dietary patterns are visualized for NAFLD severity in supplementary Fig. 3. There was no particular difference in adherence between participants without steatosis, participants with simple steatosis and participants with NASF visible.

Discussion

The first step in treating NAFLD is lifestyle modification and an important pillar in this treatment is diet. Although there is a paramount of studies on nutrition and NAFLD, there is no study yet that assesses the longitudinal association between well-known dietary quality scores, population-specific dietary pattern scores and NAFLD adjusted for important covariates such as BMI and metabolic confounders. We, therefore, examined both a priori and a posteriori dietary patterns in relation to NAFLD using comprehensive multivariable logistic mixed regression models in a large elderly white population-based cohort. After a follow-up period of almost 4.5 years, only 5% of the participants had incident NAFLD, whereas as much as 30% had regression of NAFLD. Amongst the three pre-hypothesized (a priori) dietary quality scores and the five a posteriori population-specific dietary patterns, two dietary pattern scores were associated with a lower risk of NAFLD, i.e. the WHO-score and the Traditional dietary pattern score. The latter was characterized by high intake vegetable oils & stanols, margarines or butters, potatoes, whole grains, and sweet snacks or desserts.

The three a priori dietary patterns that we studied in this paper –the Mediterranean diet, the Dutch dietary guidelines, and the world health organization diet score– largely overlapped in macro and micronutrient composition. All were highly correlated with fibre, vegetable protein, carbohydrate intake and potassium, and all were inversely correlated with animal protein, fat, sodium and haem iron intake. The only exception was the MDS, which was highly correlated with fat by its characteristic unsaturated fatty acid intake. Of the three patterns MDS is the one best-studied in relation to liver health, and accumulating evidence indeed suggests that this diet is beneficial for metabolic and liver health [12, 43]. Even though the evidence for MDS as therapeutic strategy for NAFLD predominantly originates form observational studies, the EASL consensus guidelines for NAFLD advocates the implementation of this diet as therapeutic strategy [8]. Our results are consistent with a beneficial effect of adherence to the MDS. To date, only one other longitudinal study in dietary quality has been performed [19]. This study from Ma et al. showed that adherence to the MDS was significantly associated with reduced risk of steatosis within the Framingham Heart Study. Important strengths of this study are the large sample size and the use of computed tomography. A limitation, on the other hand, was the fact that regression models were not adjusted for metabolic covariates such as diabetes and hypertension—of which we know and show in this paper—are strongly related to both NAFLD and diet.

To our knowledge, only few studies have examined the association between diet and NAFLD using factor analyses [16, 20, 21]. One of them was the cross-sectional study of Koch et al. that used 38 food groups and found an a posteriori pattern that was high in soups, beer, wine, juice, poultry and eggs and was associated with steatosis as assessed by MRI [20]. Another cross-sectional study identified four different a posteriori patterns (i.e. a fast-food, prudent, high protein, and unsaturated fatty acid pattern) using 15 food groups, and found that only the fast-food pattern was associated with ultrasound-diagnosed NAFLD [21]. Lastly, there has been one longitudinal study in adolescents (n = 995) which identified two a posteriori patterns, a healthy and a western/unhealthy pattern. This study found that adherence to the western dietary pattern at 14 years of age was associated with higher odds of NAFLD at 17 years. We did not find a detrimental association between dietary patterns and NAFLD, again, possibly because of the low number of incident NAFLD cases over time. But another important difference between our study and the others is that we adjusted for BMI, whereas they had either not taken BMI into account [20, 21], or found that their results dissipated after correction for BMI [16]. The only a posteriori pattern in our study for which we found a clear association was the traditional pattern. Interestingly, this diet resembled the macronutrient & micronutrient composition of the three a priori healthy diet scores, i.e. having a high correlation with fibre intake, mono-and disaccharide, vegetable protein, and potassium intake and an inverse correlation with fat, haem iron, sodium, and animal protein intake.

The rationale to use a posteriori dietary patterns is that they reflect the actual population-based dietary eating habits and may therefore reveal new insights in the relation between diet and disease in a particular population [17]. Together the five a posteriori dietary patterns explained almost 20% of the variation in food intake, but their independent explanatory ability was less than five percent. In addition, the five identified patterns were quite heterogeneous, which complicates unambiguous interpretation. Indeed appearing paradoxical results were obtained when comparing factor loadings and macro- and micronutrients correlation coefficients. For example, the factor loadings for the food group red meat is above 0.2 in the traditional pattern, whereas the macronutrient animal protein is negatively correlated to this pattern. There are two possible explanations for this paradox; (1) absolute intake of the food group red meat is low (median 41 g /day), which is supported by the difference in unadjusted and energy-adjusted correlation coefficients; and (2) the food group red meat is even more prominent in the red meat and alcohol pattern, as both patterns were adjusted for one another in the mixed logistic regression models, red meat is therefore not a unique feature of the traditional pattern. In other words, another combination of food groups may be more distinctive for the traditional pattern than red meat. The first explanation also reflects why adjusting for energy intake is important in this elderly population in which absolute intake is generally low. Indeed, semi-quantitative FFQs cannot measure exact energy intake and it is therefore important to look at the relative food intake. However, a diet high in energy-dense foods may not reflect the effect of the actual foods themselves, but rather an association with the high energy intake [39]. That is why we chose to show both energy-adjusted and unadjusted results.

An important characteristic of the healthy dietary patterns was fibre. Recently, a systematic umbrella review and meta-analyses, showed that high intakes of dietary fibre—from 25 g per day onwards—could prevent non-communicable diseases in the general population [44]. In addition, another population-based study showed that adherence to a plant-based diet promoted an increase in fibre-degrading bacteria in the gut. In that study, high adherence to the MDS (defined as the highest population-specific tertile in the study) was related to higher levels of short-chain fatty acid production in the faeces [45]. Indeed, butyrate production, an important short-chain fatty acid, is considered to be anti-obesogenic and has been proposed to decrease gut permeability and affect insulin sensitivity [46]. Additionally, in a recent study of our group we show that (in a larger subset of the population) animal protein was the only macronutrient that was associated with higher prevalence of NAFLD [38]. This finding is in line with other recent studies that demonstrate a detrimental effect of (particularly red) meat on liver health, insulin sensitivity, and overall mortality [47, 48]. In a spin-off study we examined whether diet-dependent acid load could explain the association between an animal protein-rich diet and NAFLD [49]. In this study, we found that an acidic diet was associated with high animal protein intake and low fruit intake as well as with NAFLD. An alkaline diet is generally accompanied by high potassium intake, which is an alkalinizing micronutrient [50]. The healthy dietary patterns in this study all had a positive correlation with potassium.

Although a major strength of our current study is that diet is taken into account as a whole, we cannot exclude the possibility that the associations found between dietary patterns and NAFLD are driven by particular food groups instead of by the entire dietary pattern [17]. The pattern salty snacks & sauces, for example, are characterised by “unhealthy” food groups that have a high sodium load, such as salty snacks and sauces. However, this pattern is also characterised by food groups that can exert specific beneficial effects on metabolic health, such as nuts and legumes [51]. Indeed, we have previously shown that specific food items, such as coffee and tea, are associated with NAFLD independent of dietary quality [52]. On the other hand, specific constituents within the diet can also be detrimental, such as haem iron from meat, which is associated to oxidative stress and insulin resistance [53]. Noteworthy is that both beneficial dietary patterns, the WHO-score and the traditional dietary pattern, had a negative correlation with vitamin E, an anti-oxidant that has been proposed as treatment of NAFLD [8]. But whereas vitamin E has been proposed to exert anti-oxidant beneficial effect on the liver [54], vitamin E has also repeatedly been associated with detrimental health outcomes such as haemorrhagic stroke [55] and mortality [56].

The large sample size and prospective design of our study enabled us to include important sociodemographic, lifestyle and metabolic confounders in the analysis. Nonetheless, there are several limitations that need mentioning. This cohort comprises an elderly, almost exclusively Caucasian population, which might hamper generalization towards other populations. Also, although none of the participants was deceased at time of follow-up, we cannot exclude the possibility that follow-up measurements were missing not at random during follow-up. In addition, although we have a large study cohort of almost a thousand participants, only a small number of incident NAFLD and regressed NAFLD was found. This might have reduced our power to detect relevant effects. In line with this, the number of NASF cases was also quite small which hindered us from performing multivariable analyses on this phenotype. However, the number of NASF cases that we found in our study is in line with the prevalence of elevated LSM in a general NAFLD population [57]. Also, the presence of steatosis could have influenced LSM, we therefore used a conservative cut-off proposed for patients with severe steatosis. This could have led to underestimation of individuals with significant fibrosis. But even with this conservative cut-off no evident difference in adherence to dietary patterns was found [36]. In addition, ultrasound and transient elastography as diagnostic tool for steatosis is not the gold standard, ultrasound is a subjective measure with a sensitivity of 79.7% and specificity of 86.2%. Diagnosis of steatosis could be subject to time of measure and operator [58]. Liver biopsy is the gold standard. However, it is ethically debatable and practically infeasible to perform an invasive procedure such as the liver biopsy in large cohorts. On the other hand, all our ultrasound measurements were performed by a single operator, which reduces the risk of bias. Furthermore, transient elastography over time is not validated in patients with NAFLD as of yet, but in hepatitis B longitudinal assessment of fibrosis using transient elastography is being used [59]. Moreover, the use of food frequency questionnaires could have led to recall bias as the questions concern dietary behaviour over the last month. In particular non-differential measurement error could have occurred (i.e. over or underreporting, as reflected in total energy intake). We have, however, dealt with the potential of this type of bias by adjusting for energy intake using the residual method and by adjusting for energy intake in the multivariable analysis to account for the extraneous variation in energy intake. In addition, previous studies have confirmed that FFQs provide sufficient information on diet to study overall dietary quality [60]. Also, BMI in elderly is a suboptimal measure for adiposity, we therefore cannot exclude the possibility that a decrease in BMI is caused by a loss in muscle mass rather than fat mass, which is generally an expression of poor nutritional and physical health [61]. Indeed, if adherence to a presumed healthy diet is low, this could also be explained by low overall food intake rather than imply that overall dietary quality is poor. In this case, low adherence to healthy diets could lead to weight loss. As the absolute consumption is low, snacking might not be detrimental at all in this frail elderly group. In line with this, as our results comprise an elderly white population, a word of caution on generalisability is therefore warranted.

In summary, in this large prospective elderly epidemiological study we found that adherence to the World Health Organization Diet and to a Traditional diet—both characterized as plant-based, high-fibre and low-fat- was related to regression of NAFLD over time. This finding is in line with increasing evidence that (red) meat is negatively associated with NAFLD and other metabolic comorbidities [47, 48]. However, we are the first longitudinal study to examine full dietary patterns with adjustment for BMI and energy intake in relation to NAFLD. While adding important new information, we believe that at the current moment, without external validation of our results and with the unknown generalizability outside our elderly population, it is too premature to firmly adapt guidelines or clinical management accordingly. Future (randomized) interventional studies on for example a meat-based vs no meat diet, ideally in younger populations, are needed. Also, there is still a gap in knowledge on the potential underlying mechanisms, such as dietary acid load or metagenomic alterations in the blood that effectuate the steatogenic effect of diet on the liver. On the other hand, to the best of our knowledge a diet that is rich in fibre and vegetables and low in red meat hasn’t been described to be detrimental for health either. And with the alarming rise in NAFLD incidence in children [62], clear and effective recommendations on the treatment of NAFLD are desperately needed. We therefore think that adherence to such a diet while awaiting the results of future studies may be beneficial.

Abbreviations

BMI:

Body mass index

CI:

Credible interval

DDG:

Dutch dietary guideline

FFQ:

Food frequency questionnaire

KPa:

Kilopascals

LSM:

Liver stiffness measurements

MDS:

Mediterranean diet score

MUFA:

Mono unsaturated fatty acids

NAFLD:

Non-alcoholic fatty liver disease

NASF:

Non-alcoholic steatofibrosis

RS:

Rotterdam study

SD:

Standard deviation

TE:

Transient elastography

WHO:

World Health Organization

References

  1. Friedman SL, Neuschwander-Tetri BA, Rinella M, Sanyal AJ. Mechanisms of NAFLD development and therapeutic strategies. Nat Med. 2018;24(7):908–22. https://doi.org/10.1038/s41591-018-0104-9.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  2. Pimpin L, Cortez-Pinto H, Negro F, et al. Burden of liver disease in Europe: epidemiology and analysis of risk factors to identify prevention policies. J Hepatol. 2018;69(3):718–35. https://doi.org/10.1016/j.jhep.2018.05.011.

    Article  PubMed  Google Scholar 

  3. Kim D, Li AA, Gadiparthi C, et al. Changing trends in etiology-based annual mortality from chronic liver disease, from 2007 through 2016. Gastroenterology. 2018;155(4):1154–63. https://doi.org/10.1053/j.gastro.2018.07.008.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Estes C, Anstee QM, Arias-Loste MT, et al. Modeling NAFLD disease burden in China, France, Germany, Italy, Japan, Spain, United Kingdom, and United States for the period 2016–2030. J Hepatol. 2018;69(4):896–904. https://doi.org/10.1016/j.jhep.2018.05.036.

    Article  PubMed  Google Scholar 

  5. Marchesini G, Brizi M, Bianchi G, et al. Nonalcoholic fatty liver disease: a feature of the metabolic syndrome. Diabetes. 2001;50(8):1844–50.

    CAS  Article  Google Scholar 

  6. Allen AM, Therneau TM, Larson JJ, Coward A, Somers VK, Kamath PS. Nonalcoholic fatty liver disease incidence and impact on metabolic burden and death: a 20 year-community study. Hepatology. 2018;67(5):1726–36. https://doi.org/10.1002/hep.29546.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. Ma J, Hwang SJ, Pedley A, et al. Bi-directional analysis between fatty liver and cardiovascular disease risk factors. J Hepatol. 2017;66(2):390–7. https://doi.org/10.1016/j.jhep.2016.09.022.

    Article  PubMed  Google Scholar 

  8. European Association for the Study of the L, European Association for the Study of D, European Association for the Study of O. EASL-EASD-EASO Clinical Practice Guidelines for the management of non-alcoholic fatty liver disease. J Hepatol. 2016;64(6):1388–402.

    Article  Google Scholar 

  9. Vilar-Gomez E, Martinez-Perez Y, Calzadilla-Bertot L, et al. Weight loss through lifestyle modification significantly reduces features of nonalcoholic steatohepatitis. Gastroenterology. 2015;149(2):367–78 (quiz e14-5).

    Article  Google Scholar 

  10. Wong VW, Wong GL, Chan RS, et al. Beneficial effects of lifestyle intervention in non-obese patients with non-alcoholic fatty liver disease. J Hepatol. 2018;69(6):1349–56.

    Article  Google Scholar 

  11. Promrat K, Kleiner DE, Niemeier HM, et al. Randomized controlled trial testing the effects of weight loss on nonalcoholic steatohepatitis. Hepatology. 2010;51(1):121–9.

    CAS  Article  Google Scholar 

  12. Zelber-Sagi S, Salomone F, Mlynarsky L. The Mediterranean dietary pattern as the diet of choice for non-alcoholic fatty liver disease: evidence and plausible mechanisms. Liver Int. 2017;37:936–49.

    CAS  Article  Google Scholar 

  13. Ryan MC, Itsiopoulos C, Thodis T, et al. The Mediterranean diet improves hepatic steatosis and insulin sensitivity in individuals with non-alcoholic fatty liver disease. J Hepatol. 2013;59(1):138–43.

    CAS  Article  Google Scholar 

  14. Properzi C, O’Sullivan TA, Sherriff JL, et al. Ad libitum Mediterranean and Low-fat diets both significantly reduce hepatic steatosis: a randomized controlled trial. Hepatology. 2018;68(5):1741–54. https://doi.org/10.1002/hep.30076.

    CAS  Article  PubMed  Google Scholar 

  15. Khalatbari-Soltani S, Imamura F, Brage S, et al. The association between adherence to the Mediterranean diet and hepatic steatosis: cross-sectional analysis of two independent studies, the UK Fenland Study and the Swiss CoLaus study. BMC Med. 2019;17(1):19. https://doi.org/10.1186/s12916-019-1251-7.

    Article  PubMed  PubMed Central  Google Scholar 

  16. Oddy WH, Herbison CE, Jacoby P, et al. The Western dietary pattern is prospectively associated with nonalcoholic fatty liver disease in adolescence. Am J Gastroenterol. 2013;108(5):778–85.

    CAS  Article  Google Scholar 

  17. Hu FB. Dietary pattern analysis: a new direction in nutritional epidemiology. Curr Opin Lipidol. 2002;13(1):3–9.

    CAS  Article  Google Scholar 

  18. Cespedes EM, Hu FB. Dietary patterns: from nutritional epidemiologic analysis to national guidelines. Am J Clin Nutr. 2015;101(5):899–900. https://doi.org/10.3945/ajcn.115.110213.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  19. Ma J, Hennein R, Liu C, et al. Improved diet quality associates with reduction in liver fat, particularly in individuals with high genetic risk scores for nonalcoholic fatty liver disease. Gastroenterology. 2018;155(1):107–17. https://doi.org/10.1053/j.gastro.2018.03.038.

    Article  PubMed  PubMed Central  Google Scholar 

  20. Koch M, Borggrefe J, Barbaresko J, et al. Dietary patterns associated with magnetic resonance imaging-determined liver fat content in a general population study. Am J Clin Nutr. 2014;99(2):369–77.

    CAS  Article  Google Scholar 

  21. Kalafati I-P, Borsa D, Dimitriou M, Revenas K, Kokkinos A, Dedoussis GV. Dietary patterns and non-alcoholic fatty liver disease in a Greek case–control study. Nutrition. 2019;61:105–10. https://doi.org/10.1016/j.nut.2018.10.032.

    CAS  Article  PubMed  Google Scholar 

  22. Ikram MA, Brusselle GGO, Murad SD, et al. The Rotterdam study: 2018 update on objectives, design and main results. Eur J Epidemiol. 2017;32(9):807–50.

    Article  Google Scholar 

  23. Boursier J, Zarski J-P, de Ledinghen V, et al. Determination of reliability criteria for liver stiffness evaluation by transient elastography. Hepatology. 2013;57(3):1182–91. https://doi.org/10.1002/hep.25993.

    Article  PubMed  Google Scholar 

  24. Goldbohm RA, van den Brandt PA, Brants HA, et al. Validation of a dietary questionnaire used in a large-scale prospective cohort study on diet and cancer. Eur J Clin Nutr. 1994;48(4):253–65.

    CAS  PubMed  Google Scholar 

  25. Feunekes IJ, Van Staveren WA, Graveland F, De Vos J, Burema J. Reproducibility of a semiquantitative food frequency questionnaire to assess the intake of fats and cholesterol in The Netherlands. Int J Food Sci Nutr. 1995;46(2):117–23.

    CAS  Article  Google Scholar 

  26. Donders-Engelen MR, Heijden Lvd, Hulshof KFAM, Vakgroep Humane Voeding LW, Zeist TNOV. Maten, gewichten en codenummers 1997. Wageningen: Vakgroep Humane Voeding; 1997.

  27. Kröger J, Ferrari P, Jenab M, et al. Specific food group combinations explaining the variation in intakes of nutrients and other important food components in the European Prospective Investigation into Cancer and Nutrition: an application of the reduced rank regression method. Eur J Clin Nutr. 2009;63:S263. https://doi.org/10.1038/ejcn.2009.85.

    Article  PubMed  Google Scholar 

  28. Trichopoulou A, Lagiou P. Healthy traditional Mediterranean diet: an expression of culture, history, and lifestyle. Nutr Rev. 1997;55(11 Pt 1):383–9.

    CAS  PubMed  Google Scholar 

  29. Hart CL, Morrison DS, Batty GD, Mitchell RJ, Davey Smith G. Effect of body mass index and alcohol consumption on liver disease: analysis of data from two prospective cohort studies. BMJ. 2010;340:c1240.

    Article  Google Scholar 

  30. (Gezondheidsraad) DHC. Dutch dietary guidelines 2015 (richtlijnen goede voeding 2015). The Hague. 2015;2015/24.

  31. WHO. https://www.who.int/news-room/fact-sheets/detail/healthy-diet. 2018.

  32. Revelle W. Psych: procedures for psychological, psychometric, and personality research. Northwestern University, Evanston, Illinois. R package version 1.9.12 (2019).

  33. Dasarathy S, Dasarathy J, Khiyami A, Joseph R, Lopez R, McCullough AJ. Validity of real time ultrasound in the diagnosis of hepatic steatosis: a prospective study. J Hepatol. 2009;51(6):1061–7.

    Article  Google Scholar 

  34. Hamaguchi M, Kojima T, Itoh Y, et al. The severity of ultrasonographic findings in nonalcoholic fatty liver disease reflects the metabolic syndrome and visceral fat accumulation. Am J Gastroenterol. 2007;102(12):2708–15.

    Article  Google Scholar 

  35. Koehler EM, Plompen EP, Schouten JN, et al. Presence of diabetes mellitus and steatosis is associated with liver stiffness in a general population: the Rotterdam study. Hepatology. 2016;63(1):138–47.

    CAS  Article  Google Scholar 

  36. Petta S, Maida M, Macaluso FS, et al. The severity of steatosis influences liver stiffness measurement in patients with nonalcoholic fatty liver disease. Hepatology. 2015;62(4):1101–10.

    Article  Google Scholar 

  37. Alberti KG, Eckel RH, Grundy SM, et al. Harmonizing the metabolic syndrome: a joint interim statement of the International Diabetes Federation Task Force on Epidemiology and Prevention; National Heart, Lung, and Blood Institute; American Heart Association; World Heart Federation; International Atherosclerosis Society; and International Association for the Study of Obesity. Circulation. 2009;120(16):1640–5.

    CAS  Article  Google Scholar 

  38. Alferink LJ, Kiefte-de Jong JC, Erler NS, et al. Association of dietary macronutrient composition and non-alcoholic fatty liver disease in an ageing population: the Rotterdam Study. Gut. 2018.

  39. Northstone K, Ness AR, Emmett PM, Rogers IS. Adjusting for energy intake in dietary pattern investigations using principal components analysis. Eur J Clin Nutr. 2008;62(7):931–8.

    CAS  Article  Google Scholar 

  40. Erler NS, Rizopoulos D, Lesaffre EMEH. JointAI: joint analysis and imputation of incomplete data in R. arXiv:1907.10867.

  41. Erler NS, Rizopoulos D, Jaddoe VW, Franco OH, Lesaffre EM. Bayesian imputation of time-varying covariates in linear mixed models. Stat Methods Med Res. 2019;28(2):555–68.

    Article  Google Scholar 

  42. Erler NS, Rizopoulos D, Rosmalen J, Jaddoe VW, Franco OH, Lesaffre EM. Dealing with missing covariates in epidemiologic studies: a comparison between multiple imputation and a full Bayesian approach. Stat Med. 2016;35(17):2955–74.

    Article  Google Scholar 

  43. Estruch R, Ros E, Salas-Salvado J, et al. Primary prevention of cardiovascular disease with a mediterranean diet supplemented with extra-virgin olive oil or nuts. N Engl J Med. 2018;378(25):e34.

    CAS  Article  Google Scholar 

  44. Reynolds A, Mann J, Cummings J, Winter N, Mete E, Te Morenga L. Carbohydrate quality and human health: a series of systematic reviews and meta-analyses. Lancet. 2019;393(10170):434–45.

    CAS  Article  Google Scholar 

  45. De Filippis F, Pellegrini N, Vannini L, et al. High-level adherence to a Mediterranean diet beneficially impacts the gut microbiota and associated metabolome. Gut. 2016;65(11):1812–21. https://doi.org/10.1136/gutjnl-2015-309957.

    CAS  Article  PubMed  Google Scholar 

  46. Carvalhana S, Machado MV, Cortez-Pinto H. Improving dietary patterns in patients with nonalcoholic fatty liver disease. Curr Opin Clin Nutr Metab Care. 2012;15(5):468–73.

    CAS  Article  Google Scholar 

  47. Zelber-Sagi S, Ivancovsky-Wajcman D, Fliss Isakov N, et al. High red and processed meat consumption is associated with non-alcoholic fatty liver disease and insulin resistance. J Hepatol. 2018;68(6):1239–46.

    CAS  Article  Google Scholar 

  48. Etemadi A, Sinha R, Ward MH, et al. Mortality from different causes associated with meat, heme iron, nitrates, and nitrites in the NIH-AARP Diet and Health Study: population based cohort study. BMJ. 2017;357:j1957.

    Article  Google Scholar 

  49. Alferink LJM, Kiefte-de Jong JC, Erler NS, et al. Diet-dependent acid load—the missing link between an animal protein-rich diet and non-alcoholic fatty liver disease? J Clin Endocrinol Metab. 2019;104:6325–37.

    Article  Google Scholar 

  50. Remer T, Manz F. Potential renal acid load of foods and its influence on urine pH. J Am Diet Assoc. 1995;95(7):791–7.

    CAS  Article  Google Scholar 

  51. Martinez R, Lopez-Jurado M, Wanden-Berghe C, Sanz-Valero J, Porres JM, Kapravelou G. Beneficial effects of legumes on parameters of the metabolic syndrome: a systematic review of trials in animal models. Br J Nutr. 2016;116(3):402–24.

    CAS  Article  Google Scholar 

  52. Alferink LJM, Fittipaldi J, Kiefte-de Jong JC, et al. Coffee and herbal tea consumption is associated with lower liver stiffness in the general population: the Rotterdam study. J Hepatol. 2017;67(2):339–48.

    CAS  Article  Google Scholar 

  53. Romeu M, Aranda N, Giralt M, Ribot B, Nogues MR, Arija V. Diet, iron biomarkers and oxidative stress in a representative sample of Mediterranean population. Nutr J. 2013;12(1):102. https://doi.org/10.1186/1475-2891-12-102.

    Article  PubMed  PubMed Central  Google Scholar 

  54. Sanyal AJ, Chalasani N, Kowdley KV, et al. Pioglitazone, Vitamin E, or Placebo for Nonalcoholic Steatohepatitis. N Engl J Med. 2010;362(18):1675–85. https://doi.org/10.1056/NEJMoa0907929.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  55. Schürks M, Glynn RJ, Rist PM, Tzourio C, Kurth T. Effects of vitamin E on stroke subtypes: meta-analysis of randomised controlled trials. BMJ. 2010;341:c5702. https://doi.org/10.1136/bmj.c5702.

    Article  PubMed  PubMed Central  Google Scholar 

  56. Bjelakovic G, Nikolova D, Gluud LL, Simonetti RG, Gluud C. Mortality in randomized trials of antioxidant supplements for primary and secondary prevention: systematic review and meta-analysis. JAMA. 2007;297(8):842–57. https://doi.org/10.1001/jama.297.8.842.

    CAS  Article  PubMed  Google Scholar 

  57. Singh S, Allen AM, Wang Z, Prokop LJ, Murad MH, Loomba R. Fibrosis progression in nonalcoholic fatty liver vs nonalcoholic steatohepatitis: a systematic review and meta-analysis of paired-biopsy studies. Clin Gastroenterol Hepatol. 2015;13(4):643–54 (e1-9; quiz e39-40).

    Article  Google Scholar 

  58. Li Q, Dhyani M, Grajo JR, Sirlin C, Samir AE. Current status of imaging in nonalcoholic fatty liver disease. World J Hepatol. 2018;10(8):530–42.

    Article  Google Scholar 

  59. Wu SD, Liu LL, Cheng JL, et al. Longitudinal monitoring of liver fibrosis status by transient elastography in chronic hepatitis B patients during long-term entecavir treatment. Clin Exp Med. 2018;18(3):433–43.

    CAS  Article  Google Scholar 

  60. Hu FB, Rimm E, Smith-Warner SA, et al. Reproducibility and validity of dietary patterns assessed with a food-frequency questionnaire. Am J Clin Nutr. 1999;69(2):243–9.

    CAS  Article  Google Scholar 

  61. Trajanoska K, Schoufour JD, Darweesh SK, et al. Sarcopenia and its clinical correlates in the general population: the Rotterdam study. J Bone Miner Res. 2018;33(7):1209–18. https://doi.org/10.1002/jbmr.3416.

    Article  PubMed  Google Scholar 

  62. Nobili V, Alisi A, Valenti L, Miele L, Feldstein AE, Alkhouri N. NAFLD in children: new genes, new diagnostic modalities and new drugs. Nat Rev Gastroenterol Hepatol. 2019;16(9):517–30.

    Article  Google Scholar 

  63. Lüdtke O, Robitzsch A, West SG. Analysis of interactions and nonlinear effects with missing data: a factored regression modeling approach using maximum likelihood estimation. Multivar Behav Res. 2019. https://doi.org/10.1080/00273171.2019.1640104.

    Article  Google Scholar 

  64. Bartlett JW, Seaman SR, White IR, Carpenter JR. Alzheimer’s disease neuroimaging I. Multiple imputation of covariates by fully conditional specification: accommodating the substantive model. Stat Methods Med Res. 2015;24(4):462–87.

    Article  Google Scholar 

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Acknowledgements

The authors thank the Rotterdam Study participants and staff; in particular the collaborating general practitioners and pharmacists. The authors are also deeply grateful to Mrs. van Wijngaarden (nurse ultrasonographist) for performing the abdominal ultrasonography and transient elastography.

Funding

The Rotterdam Study is supported by the Erasmus MC University Medical Centre and Erasmus University Rotterdam, the Netherlands Organization for Scientific Research (NWO), the Netherlands Organization for Health Research and Development (ZonMw), the Research Institute for Diseases in the Elderly (RIDE), the Ministry of Education, Culture and Science, the Ministry of Health, Welfare and Sports, the European Commission (DG XII) and by the Municipality of Rotterdam. JCK received a grant from the Den Dulk-Moermans foundation (Leiden University Fund).

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Authors and Affiliations

  1. Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands

    Louise J. M. Alferink, Robert J. de Knegt, Herold J. Metselaar & Sarwa Darwish Murad

  2. Department of Biostatistics, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands

    Nicole S. Erler

  3. Toronto Centre of Liver Disease, Toronto General Hospital, University Health Network, Toronto, Canada

    Harry L. A. Janssen

  4. Department of Epidemiology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands

    Jessica C. Kiefte-de Jong

  5. Department of Public Health and Primary Care/LUMC Campus The Hague, Leiden University Medical Center, Postzone VO-P, Postbus 9600, 2300 RC, Leiden, The Netherlands

    Jessica C. Kiefte-de Jong

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Conceptualization: Louise JM Alferink, Sarwa Darwish Murad, Jessica C. Kiefte-deJong; Methodology: Nicole S Erler, Jessica C. Kiefte-deJong; Formal analysis and investigation: Louise JM Alferink, Nicole S Erler; Writing—original draft preparation: Louise JM Alferink; Writing—review and editing: Nicole S Erler, Sarwa Darwish Murad, Jessica C Kiefte-deJong; Funding acquisition: Harry LA Janssen; Resources: Robert J De Knegt; Supervision: Herold J Metselaar.

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Alferink, L.J.M., Erler, N.S., de Knegt, R.J. et al. Adherence to a plant-based, high-fibre dietary pattern is related to regression of non-alcoholic fatty liver disease in an elderly population. Eur J Epidemiol 35, 1069–1085 (2020). https://doi.org/10.1007/s10654-020-00627-2

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  • DOI: https://doi.org/10.1007/s10654-020-00627-2

Keywords

  • Hepatic steatosis
  • Dietary patterns
  • Mediterranean
  • Plant-based