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Genotoxic and morpho-physiological responses of ZnO macro- and nano-forms in plants

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Abstract

In the current study, two plants, viz., Pisum sativum L. and Hordeum vulgare L., were exposed to nano- and macro-dispersed ZnO at 1, 10, and 30 times of maximal permissible concentration (MPC). The main objective of the study is to depict and compare the genotoxicity in terms of chromosomal anomalies, cytotoxicity (i.e., mitotic index), and phytotoxicity (viz., germination, morphometry, maximal quantum yield, and chlorophyll fluorescence imaging) of macro- and nano-forms of ZnO along with their accumulation and translocation. In the case of genotoxic and cytotoxic responses, the maximal effect was observed at 30 MPC, regardless of the macro- or nano-forms of ZnO. The phytotoxic observations revealed that the treatment with macro- and nano-forms of ZnO significantly affected the germination rate, germination energy, and length of roots and shoots of H. vulgare in a dose-dependent manner. The factor toxicity index of treated soil demonstrated that toxicity soared as concentrations increased and that at 30 MPC, toxicity was average and high in macro- and nano-dispersed ZnO, respectively. Furthermore, the photosynthetic parameters were observed to be negatively affected in both treatments, but the maximal effect was observed in the case of nano-dispersed form. It was noted that the mobility of nano-dispersed ZnO in the soil was higher than macro-dispersed. The increased mobility of nano-dispersed ZnO might have boosted their accumulation and translocation that subsequently led to the oxidative stress due to the accelerated production of reactive oxygen species, thus strengthen toxicity implications in plants.

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Acknowledgements

The study was supported by the grant from the Russian Science Foundation (project No. 21-77-20089) at the Southern Federal University.

Funding

The study was supported by the grant from the Russian Science Foundation (project No. 21-77-20089) at the Southern Federal University.

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  1. Southern Federal University, Rostov-On-Don, Russia, 344006

    Arpna Kumari, Vasiliy A. Chokheli, Vladimir S. Lysenko, Saglara S. Mandzhieva, Tatiana M. Minkina, Mahmoud Mazarji, Vishnu D. Rajput, Victoria A. Shuvaeva, Svetlana S. Sushkova & Anatoly Barakhov

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  1. Arpna Kumari
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Contributions

Conceptualization and Methodology were contributed by SSM and TMM; experiment performing was contributed by VAC, VL, AK, MM, and AB; writing—original draft was contributed by AK; writing—review and editing was contributed by AK, VDR, SSM, VAS, SS, and TMM; All authors have read and agreed to the published version of the manuscript.

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Kumari, A., Chokheli, V.A., Lysenko, V.S. et al. Genotoxic and morpho-physiological responses of ZnO macro- and nano-forms in plants. Environ Geochem Health 45, 9345–9357 (2023). https://doi.org/10.1007/s10653-022-01428-0

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