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The residue of tetracycline antibiotics in soil and Brassica juncea var. gemmifera, and the diversity of soil bacterial community under different livestock manure treatments

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Abstract

Tetracycline antibiotics (TCs) are a broad-spectrum antibiotic, widely used in livestock and poultry breeding. Residue of tetracycline antibiotics in animal manure may cause changes in vegetable TCs content and soil microbial community. On the basis of the investigation and analysis of TCs pollution in the soil of main vegetable bases and the livestock manure of major large-scale farms in Chongqing, China, field experiment was conducted to study the residues of tetracycline antibiotics in Brassica juncea var. gemmifera and soil under different kinds and different dosages of livestock manures. Effects of tetracycline antibiotics on the structure and diversity of soil microbial community were also investigated by high-throughput sequencing. TCs content in soil was increased by applying livestock manure. The contents of tetracycline, oxytetracycline (OTC) and chlortetracycline (CTC) in the soil under pig manure treatment were 171.07–660.20 μg kg−1, 25.38–345.78 μg kg−1 and 170.77–707.47 μg kg−1, respectively. The contents of TC, OTC and CTC in the soil under the treatment of chicken manure were 166.62–353.61 μg kg−1, 122.25–251.23 μg kg−1 and 15.12–80.91 μg kg−1, respectively. TCs in edible parts of Brassica juncea var. gemmifera was increased after livestock manure treatment Proteobacteria, Acidobacteria, Actinobacteria, Chioroflexi and Bacteroidetes under livestock manure treatment were the dominant phyla, accounting for 85.2–92.4% of the total abundance of soil bacteria. The soil OTUs under the treatment of pig manure was higher than that under the treatment of chicken manure. Biogas residue (Livestock manure after fermentation treatment) can effectively reduce the environmental and ecological risks caused by antibiotic residues.

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Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We would like to express our gratitude to the reviewers of this manuscript; their insight was instrumental to improving this paper. Finally, we also thank Ministry of Agriculture and Rural Affairs of the People's Republic of China for the financial support necessary to make all of this work possible.

Funding

This study was funded by Fund of China Agriculture Research System (CARS-23-13B), and the National Key Research and Development Program of China (2018YFD0201200).

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  1. L. S. Xu and W. Z. Wang have contributed equally to this work and are co-first authors.

Authors and Affiliations

  1. College of Resources and Environmental Sciences, Southwest University, Chongqing, 400715, People’s Republic of China

    L. S. Xu, W. Z. Wang, J. B. Deng & W. H. Xu

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  1. L. S. Xu
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  2. W. Z. Wang
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  3. J. B. Deng
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  4. W. H. Xu
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Contributions

LSX and WHX conceived the study. WZW collected the data presented in the manuscript. LSX, WZW and JBD performed statistical analyses. All authors contributed to the writing and revision of the final manuscript. All authors read and approved the final manuscript.

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Correspondence to W. H. Xu.

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The sampling of soil and vegetable did not require permission and the species are not classified as endangered, and are not under any protection in any of the sampled areas.

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Xu, L.S., Wang, W.Z., Deng, J.B. et al. The residue of tetracycline antibiotics in soil and Brassica juncea var. gemmifera, and the diversity of soil bacterial community under different livestock manure treatments. Environ Geochem Health 45, 7–17 (2023). https://doi.org/10.1007/s10653-022-01213-z

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