Soil eaten by chacma baboons adsorbs polar plant secondary metabolites representative of those found in their diet
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Geophagy, the deliberate consumption of earth materials, is common among humans and animals. However, its etiology and function(s) remain poorly understood. The major hypotheses about its adaptive functions are the supplementation of essential elements and the protection against temporary and chronic gastrointestinal (GI) distress. Because much less work has been done on the protection hypothesis, we investigated whether soil eaten by baboons protected their GI tract from plant secondary metabolites (PSMs) and described best laboratory practices for doing so. We tested a soil that baboons eat/preferred, a soil that baboons never eat/non-preferred, and two clay minerals, montmorillonite a 2:1 clay and kaolinite a 1:1 clay. These were processed using a technique that simulated physiological digestion. The phytochemical concentration of 10 compounds representative of three biosynthetic classes of compounds found in the baboon diet was then assessed with and without earth materials using high-performance liquid chromatography with diode-array detection (HPLC–DAD). The preferred soil was white, contained 1% halite, 45% illite/mica, 14% kaolinite, and 0.8% sand; the non-preferred soil was pink, contained 1% goethite and 1% hematite but no halite, 40% illite/mica, 19% kaolinite, and 3% sand. Polar phenolics and alkaloids were generally adsorbed at levels 10× higher than less polar terpenes. In terms of PSM adsorption, the montmorillonite was more effective than the kaolinite, which was more effective than the non-preferred soil, which was more effective than the preferred soil. Our findings suggest that HPLC–DAD is best practice for the assessment of PSM adsorption of earth materials due to its reproducibility and accuracy. Further, soil selection was not based on adsorption of PSMs, but on other criteria such as color, mouth feel, and taste. However, the consumption of earth containing clay minerals could be an effective strategy for protecting the GI tract from PSMs.
KeywordsPlant toxin adsorption Simulated digestion HPLC–DAD Soil eating Detoxification Pica Methods
This work was funded by a Natural Science and Engineering Research Council grant to JT Arnason. We thank Cape Nature and Jennifer Giddy for the opportunity to conduct research in South Africa and Nimal De Silva, Jean Bjornson, and Christopher N. Boddy for assistance with the analytical analyses. We also thank the EGH editor, Professor William Mahaney, and two anonymous reviewers for helpful comments on the previous version of the manuscript.
- Abrahams, P. W. (2013). Geophagy and the involuntary ingestion of soil. In Essentials of medical geology (pp. 433–454). Berlin: Springer.Google Scholar
- Aufreiter, S., Mahaney, W. C., Milner, M. W., Huffman, M. A., Hancock, R. G., Wink, M., et al. (2001). Mineralogical and chemical interactions of soils eaten by chimpanzees of the Mahale Mountains and Gombe Stream National Parks, Tanzania. Journal of Chemical Ecology, 27(2), 285–311.CrossRefGoogle Scholar
- Cornell, R. M., & Schwertmann, U. (2006). The iron oxides structure, properties, reactions, occurrences and uses. Weinheim: Wiley-VCH. http://nbn-resolving.de/urn:nbn:de:101:1-2014081514273. Accessed 1 June 2017.
- Espinosa Gómez, F., Santiago García, J., Gómez Rosales, S., Wallis, I. R., Chapman, C. A., Morales Mávil, J., et al. (2015). Howler monkeys (Alouatta palliata mexicana) produce tannin-binding salivary proteins. International Journal of Primatology, 36(6), 1086–1100. doi: 10.1007/s10764-015-9879-4.CrossRefGoogle Scholar
- Harris, C. S., Burt, A. J., Saleem, A., Le, P. M., Martineau, L. C., Haddad, P. S., et al. (2007). A single HPLC-PAD-APCI/MS method for the quantitative comparison of phenolic compounds found in leaf, stem, root and fruit extracts of Vaccinium angustifolium. Phytochemical Analysis, 18(2), 161–169. doi: 10.1002/pca.970.CrossRefGoogle Scholar
- Jeannoda, V., Rakotonirina, O., Randrianarivo, H., Rakoto, D., Wright, P. C., & Hladik, C. M. (2003). The toxic principle of the bamboo eaten by Hapalemur aureus is not neutralized by soil consumption. Revue d’Ecologie, 58, 151–153.Google Scholar
- Lounasmaa, M., & Tamminen, T. (1993). Chapter 1: The tropane Alkaloids. In The Alkaloids: Chemistry and pharmacology (vol. 44, pp. 1–114). Elsevier. doi: 10.1016/S0099-9598(08)60143-1.
- Mahaney, W. C., Zippin, J., Milner, M. W., Sanmugadas, K., Hancock, R. G. V., Aufreiter, S., et al. (1999). Chemistry, mineralogy and microbiology of termite mound soil eaten by the chimpanzees of the Mahale Mountains, Western Tanzania. Journal of Tropical Ecology, 15, 565–588.CrossRefGoogle Scholar
- Pebsworth, P. A., MacIntosh, A. J. J., Morgan, H. R., & Huffman, M. A. (2012b). Factors influencing the ranging behavior of chacma baboons (Papio hamadryas ursinus) living in a human-modified habitat. International Journal of Primatology, 33(4), 872–887. doi: 10.1007/s10764-012-9620-5.CrossRefGoogle Scholar
- Strier, K. B. (2007). Primate behavioral ecology (3rd ed.). Boston: Pearson Allyn and Bacon.Google Scholar
- US Pharmacopeia. (2017). http://www.pharmacopeia.cn/v29240/usp29nf24s0_ris1s126.html. Accessed 1 June 2017.
- Wakibara, J. V., Huffman, M. A., Wink, M., Reich, S., Aufreiter, S., Hancock, R. G. V., et al. (2001). The Adaptive significance of geophagy for Japanese macaques (Macaca fuscata) at Arashiyama, Japan. International Journal of Primatology, 22(3), 495–520. doi: 10.1023/A:1010763930475.CrossRefGoogle Scholar
- Young, S. L., Wilson, M. J., Hillier, S., Delbos, E., Ali, S. M., & Stoltzfus, R. J. (2010). Differences and commonalities in physical, chemical and mineralogical properties of Zanzibari geophagic soils. Journal of Chemical Ecology, 36(1), 129–140. doi: 10.1007/s10886-009-9729-y.CrossRefGoogle Scholar