Abstract
In a multifactorial pot experiment, maize (Zea mays L.) with or without inoculation with the arbuscular mycorrhizal (AM) fungus Glomus mosseae BEG167 was grown in a sterilized soil spiked with three levels of zinc (0, 300 and 900 mg Zn kg−1 soil) and three levels of cadmium (0, 25 and 100 mg Cd kg−1 soil). At harvest after 8 weeks of growth, the proportion of root length of inoculated plants colonized decreased with increasing Zn or Cd additon, and was 56% in the absence of both metals and was reduced significantly to 27% in the presence of the higher levels of both metals. Mycorrhizal plants had higher biomass than non-mycorrhizal controls except at the highest soil level of Cd. Cadmium had more pronounced effects on plant biomass than did Zn at the levels studied and the two metals showed a significant interaction. The data suggest that mycorrhizal inoculation increased plant growth with enchancement of P nutrition, perhaps increasing plant tolerance to Zn and Cd by a dilution effect. AM inoculation also led to higher soil solution pH after harvest, possibly reducing the availability of the metals for plant uptake, and lowered the concentrations of soluble Zn and Cd in the soil solution, perhaps by adsorption onto the extrametrical mycelium.
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References cited
Bi YL, Li XL, Christie P, (2003). Influence of early stages of arbuscular mycorrhiza on uptake of zinc and phosphorus by red clover from a low-phosphorus soil amended with zinc and phosphorus Chemosphere 50:831–837
Bradley R, Burt AJ, Read DJ, (1981). Mycorrhizal infection and resistance to heavy metal toxicity in Calluna vulgaris Nature, Lond 292:335–337
Chen BD, Li XL, Tao HQ, Christie P, Wong MH, (2003). The role of arbuscular mycorrhiza in zinc uptake by red clover growing in a calcareous soil spiked with various quantities of zinc Chemosphere 50:839–846
Cooper KM, Tinker PB, (1978). Translocation and transfer of nutrients in vesicular-arbuscular mycorrhizas. II. Uptake and translocation of phosphorus, zinc and sulphur New Phytol 81:43–52
El-Kherbawy M, Angle JS, Heggo A, Chaney RL, (1989). Soil pH, rhizobia and vesicular–arbuscular mycorrhizae inoculation effects on growth and heavy metal uptake of alfalfa (Medicago sativa L.) Biol Fertil Soils 8:61–65
Gildon A, Tinker PB, (1981). A heavy metal tolerant strain of a mycorrhizal fungus Trans Br Mycol Soc 77:648–649
Gildon A, Tinker PB, (1983). Interactions of vesicular-arbuscular mycorrhizal infection and heavy metals in plants. I. The effects of heavy metals on the development of vesicular–arbuscular mycorrhizas New Phytol 95:247–261
Heggo A, Angle JS, Chaney RL, (1990). Effects of vesicular–arbuscular mycorrhizal fungi on heavy metal uptake by soybeans Soil Biol Biochem 22:865–869
Ietswaart JH, Griffioen WAJ, Ernst WHO, (1992). Seasonality of VAM infection in three populations of Agrostis capillaris (Gramineae) on soil with or without heavy metal enrichment Plant Soil 139:67–73
Jakobsen I, Abbott LK, Robson AD, 1992a External hyphae of arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 1. Spread of hyphae and phosphorus inflow into roots New Phytol 120:371–380
Jakobsen I, Abbott LK, Robson AD, 1992b External hyphae of arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 2. Hyphal transport of 32P over defined distances New Phytol 120:509–516
Jamal A, Ayub N, Usman M, Khan AG, (2002). Arbuscular mycorrhizal fungi enchance zinc and nickel uptake from contaminated soil by soybean and lentil Int J Phytorem 4:205–221
Joner EJ, Leyval C, (1997). Uptake of 109Cd by roots and hyphae of a Glomus mosseae/Trifolium subterraneum mycorrhiza from soil amended with high and low concentrations of cadmium New Phytol 135:353–360
Killham K, Firestone MK, (1983). Vesicular arbuscular mycorrhizal mediation of grass response to acidic and heavy metal depositions Plant Soil 72:39–48
Koomen I, McGrath SP, Giller KE, (1990). Mycorrhizal infection of clover is delayed in soils contaminated with heavy metals from past sewage sludge applications Soil Biol Biochem 22:871–873
Kormanik PP, McGraw AC, (1982). Quantification of vesicular-arbuscular mycorrhizae in plant roots In Schenck NC, (Eds) Methods and Principles of Mycorrhizal Research St Paul, MN Am Phytopath Soc 37–45
Kothari SK, Marschner H, Römheld V, (1991). Contribution of the VA mycorrhizal hyphae in acquisition of phosphorus and zinc by maize grown in a calcareous soil Plant Soil 131:177–185
Leyval C, Turnau K, Haselwandter K, (1997). Effect of heavy metal pollution on mycorrhizal colonization and function: Physiological, ecological and applied aspects Mycorrhiza 7:139–153
Li XL, Christie P, (2000). Changes in soil solution Zn and pH and uptake of Zn by arbuscular mycorrhizal red clover in Zn-contaminated soil Chemosphere 42:201–207
Li XL, George E, Marschner H, 1991a Extension of the phosphorus depletion zone in VA mycorrhizal white clover in a calcareous soil Plant Soil 136:41–48
Li XL, George E, Marschner H, 1991b Phosphorus depletion and pH decrease at the root–soil and hyphae–soil interfaces of VA mycorrhizal white clover fertilized with ammonium New Phytol 119:397–404
Li XL, George E, Marschner H, Zhang JL, (1997). Phosphorus acquisition from compacted soil by hyphae of a mycorrhizal fungus associated with red clover (Trifolium pratense) Can J Bot 75:723–729
Liu A, Hamel C, Hamilton RI, Ma BL, Smith DL, (2000). Acquisition of Cu, Zn, Mn, and Fe by mycorrhizal maize (Zea mays L.) grown in soil at different P and micronutrient levels Mycorrhiza 9:331–336
Schüepp H, Dehn B, Sticher H, (1987). Interaktionen Zwischen VA-Mykorrhizen und Schwermetallbelastungen Angew Bot 61:85–96
Shetty KG, Banks MK, Hetrick BAD, Schwab AP, 1994a Biological characterization of a southeast Kansas mining site Water Air Soil Pollut 78:169–177
Shetty KG, Hetrick BAD, Figge DAH, Schwab AP, 1994b Effects of mycorrhizae and other soil microbes on revegetation of heavy metal contaminated mine spoil Environ Pollut 86:181–188
Smith FA, Smith SE, (1997). Structural diversity in (vesicular)-arbuscular mycorrhizal symbioses New Phytol 137:373–388
Turnau K, Kottke I, Oberwinkler F, (1993). Element localization in mycorrhizal roots of Pteridium aquilinum (L) Kuhn collected from experimental plots treated with cadmium dust New Phytol 123:313–324
Turnau K, Miszalski Z, Trouvelot A, Bonfante P, Gianinazzi S, (1996). Oxalis acetosella as a monitoring plant on highly polluted soils In Azcon-Aguilar C, Barea JM, (Eds) Mycorrhizas in Integrated Systems: from Genes to Plant Development Luxembourg European Commission EUR 16728 483–486
Weissenhorn I, Glashoff A, Leyval C, Berthelin J, (1994). Differential tolerance to Cd and Zn of arbuscular mycorrhizal (AM) fungal spores isolated from heavy metal polluted and unpolluted soils Plant Soil 167:189–196
Weissenhorn I, Leyval C, (1995). Root colonization of maize by a Cd-sensitive and a Cd-tolerant Glomus mosseae and cadmium uptake in sand culture Plant Soil 175:233–238
Weissenhorn I, Leyval C, Berthelin J, (1993). Cd-tolerant arbuscular mycorrhizal (AM) fungi from heavy-metal polluted soils Plant Soil 157:247–256
Weissenhorn I, Mench M, Leyval C, (1995). Biovailability of heavy metals and arbuscular mycorrhizas in a sewage sludge amended sandy soil Soil Biol Biochem 27:287–296
Zhu YG, Christie P, Laidlaw AS, (2001). Uptake of Zn by arbuscular mycorrhizal white clover from Zn-contaminated soil Chemosphere 42:193–199
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We thank the National Science Foundation of China (Projects 30370818 and 30230250) and the Royal Society (China Exchanges Project 15360) for financial support.
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Shen, H., Christie, P. & Li, X. Uptake of zinc, cadmium and phosphorus by arbuscular mycorrhizal maize (Zea mays L.) from a low available phosphorus calcareous soil spiked with zinc and cadmium. Environ Geochem Health 28, 111–119 (2006). https://doi.org/10.1007/s10653-005-9020-2
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DOI: https://doi.org/10.1007/s10653-005-9020-2