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Effects of salinity stress on anxiety behavior and antioxidant capability of guppy (Poecilia reticulata)

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Abstract

The objective of this study was to determine the effect of salinity on anxiety behavior and liver antioxidant capacity in the guppy (Poecilia reticulata). Guppies were exposed to salinities of 0‰, 5‰, 10‰, 15‰ and 20‰ for acute stress tests, and then we analyzed the activity of antioxidant enzymes at 3, 6, 12, 24, 48, 72 and 96 h. During the experiment, the anxiety behavior of guppy was enhanced at salinities of 10‰, 15‰, and 20‰, as evidenced by a significantly higher latency time for the first passage through the upper part than that of the control group (P < 0.05). CAT activity was highest at 24 h in the treatment with the salinity of 10‰, and SOD and GPX activities were highest at 12 h into the treatment with the salinity of 10‰. The SOD and CAT activities were significantly higher than the control group after 96 h of treatment at different salinities (P < 0.05). The MDA contents of the experimental groups at salinities of 5‰ and 10‰ were not significantly different from the control group after 96 h of treatment (P > 0.05). While the MDA contents of the experimental groups at salinities of 15‰ and 20‰ were still significantly higher than the control group after 96 h of treatment (P < 0.05). The experimental results indicated that elevated salinity could lead to oxidative stress in the guppy, altering their anxiety behavior as well as the activity of antioxidant enzymes. In conclusion, drastic changes in salinity during culture should be avoided.

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The data used in this study are available from the corresponding author.

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Acknowledgements

This work was supported by the National Natural Science Foundation (China, 32002405) and the Henan Natural Science Foundation (202300410130).

Author contributions

MX: Data curation, methodology, writing-original draft. CZ: Supervision, project administration, writing-reviewing and editing. QQ: Data curation, writing-reviewing and editing, validation. RW: Conceptualization, writing-reviewing and editing, validation. SZ: Writing-reviewing and editing, validation. RY: Writing-reviewing and editing, validation. BL: Writing-reviewing and editing, validation. SL: Writing-reviewing and editing, validation.

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Authors and Affiliations

  1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471003, China

    Mingjia Xu, Chunnuan Zhang, Qian Qi, Renpeng Wang, Shibo Zhang, Runkun Yan, Bin Li & Shengnan Li

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  1. Mingjia Xu
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  2. Chunnuan Zhang
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Correspondence to Chunnuan Zhang.

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All experimental animal procedures used in this study were approved by the Animal Care and Ethics Committee of Henan University of Science and Technology.

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Xu, M., Zhang, C., Qi, Q. et al. Effects of salinity stress on anxiety behavior and antioxidant capability of guppy (Poecilia reticulata). Ecotoxicology 32, 598–605 (2023). https://doi.org/10.1007/s10646-023-02667-2

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