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Acute toxicity assays using Danio rerio and Daphnia magna to assess hot-spring drainage in the Shibukuro and Tama Rivers (Akita, Japan)

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Abstract

We investigated the lethal toxicity of Shibukuro and Tama river water near the inflow of Tamagawa hot-spring water in Akita Prefecture, Japan. We first measured metal concentrations in both rivers. We detected iron, arsenic, and aluminum; the concentrations of each tended to decrease from upstream to downstream. We next examined the influence of river water on zebrafish Danio rerio and water flea Daphnia magna. We observed lethal effects in both species, with Daphnia magna more sensitive to toxicity than Danio rerio. For both species, the toxic effects of river water decreased with increasing distance downstream from the inflow of hot-spring water. Our results show that the metals discharged from Tamagawa hot spring have a negative effect on aquatic organisms.

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Funding

This study was supported in part by a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Grant-in-Aid for Scientific Research [B] no. 19H04294) to Y.H.

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Contributions

All authors listed in the current study contributed to the experimental design or data analysis. (KS: Sampling and Bioassay; CT: Chemical analysis; YH: All experiments except chemical analysis).

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Correspondence to Yoshifumi Horie.

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The authors declare no competing interests.

Animal research

The fish which was used in the present study were handled according to the guidelines of Akita Prefectural University and Kobe University.

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This research did not involve human subjects, so clinical trial registration is not applicable.

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The authors certify that this manuscript is our original unpublished work, has not been published elsewhere, and is not under consideration by another journal. All authors have approved the manuscript and agree with its submission.

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Suwa, K., Takahashi, C. & Horie, Y. Acute toxicity assays using Danio rerio and Daphnia magna to assess hot-spring drainage in the Shibukuro and Tama Rivers (Akita, Japan). Ecotoxicology 31, 187–193 (2022). https://doi.org/10.1007/s10646-021-02514-2

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