Abstract
Realising the hazardous effect of nickel on human health, microbes and plants are effectively used for bioremediation. The endophytic microorganisms have an important role in the phytoremediation of nickel using Vigna radiata. Therefore, in order to harness the potential of microbial strains, the present study was designed to examine the metal biosorption ability of endophytic bacterial strains isolated from plants growing in nickel-contaminated soil. A total of six endophytic nickel resistance bacteria were isolated from the plant Vigna radiata. The metal tolerant bacterial strains were identified following 16 S rRNA gene sequence analysis. Nickel biosorption estimation and plant growth-promoting (PGP) activities of isolated strains were performed and found high nickel biosorption efficiency of 91.3 ± 0.72% at 600 mg L−1 using Bacillus safensis an isolated endophytic strain from Vigna radiata. Furthermore, high indole acetic acid (IAA) and exopolysaccharide (EPS) production were obtained in all the strains as compared to without nickel-containing medium used as control. Moreover, the production of high EPS suggests improved biosorption ability of isolated endophytic strains. In addition, a kinetic study was also performed to evaluate different adsorptions isotherms and support the nickel biosorption ability of endophytic strains. The treatment of nickel electroplating industrial effluent was also demonstrated by isolated endophytic strains. Among six (6) strains, B. cereus showed maximum 57.2 ± 0.62% biosorption efficiency of nickel which resulted in the removal of 1003.50 ± 0.90 mg L−1 of nickel from the electroplating industry effluents containing initial 1791 ± 0.90 mg L−1 of nickel. All other strains were also capable of significant nickel biosorption from electroplating industry effluents as well. Thus, isolated endophytic nickel tolerant strains can be further used at large-scale biosorption of nickel from electroplating industry effluent.
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PV has played a vital role in the conceptualization of research ideas. SK has conducted the laboratory work and prepared the rough draft of the manuscript. RC and BK have performed the formal analysis of the results and writing of the MS. The experimental, writing, and formal analysis were supervised by PV. Also, PV and RC roles are key in the acquisition of the financial supports for the project leading to this publication. All authors have approved the final version of the manuscript.
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This work was financially supported by the Department of Biotechnology, Government of India through Project BT/304/NE/TBP/2012 and BT/PR7333/PBD/26/373/2012. The work was also supported by the Department of Science and Technology through Project IFA/2012/EAS-05 and DST-FIST (SR/FST/LSI-676/2016). B.K. acknowledges Jawaharlal Nehru Memorial Fund, New Delhi, and CSIR-SRF for providing funding for Doctoral Studies.
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Kashyap, S., Chandra, R., Kumar, B. et al. Biosorption efficiency of nickel by various endophytic bacterial strains for removal of nickel from electroplating industry effluents: an operational study. Ecotoxicology 31, 565–580 (2022). https://doi.org/10.1007/s10646-021-02445-y
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DOI: https://doi.org/10.1007/s10646-021-02445-y