Abstract
Many organic pollutants attract public health concern due to their genotoxicity. To investigate the genotoxicity of organic matter in surface water of the Pearl River Delta (PRD). Organic substances of 24 samples (dry and wet season) from North River, West River and East River were extracted from 60 L source water by XAD-2 macroporous resin. DNA damage effect of organic extracts was tested in human derived liver cells (HL-7702), using single cell gel electrophoresis (SCGE) assay. The results showed that 100% organic extracts (24/24) could induce DNA damage in HL-7702 cells when the concentration was above 1.0 L surface water/ml culture, no significant difference of DNA damage between dry and wet seasons was observed. The organic substance-induced DNA damage in HL-7702 cells was significantly (P < 0.05) correlated with the contents of Dissolved Organic Carbon in both seasons and Total Suspended Solids in dry season. In conclusion, organic extracts induced genetic damage in HL-7702 cells, indicating potential genotoxicity of organic pollutants of surface water from PRD, South China.
Similar content being viewed by others
References
Abrams JF, Hohn S, Rixen T, Baum A, Merico A (2016) The impact of Indonesian peatland degradation on downstream marine ecosystems and the global carbon cycle. Global Change Biol 22:325–337
An TC, Qiao M, Li GY, Sun HW, Zeng XY, Fu JM (2011) Distribution, sources, and potential toxicological significance of PAHs in drinking water sources within the Pearl River Delta. J Environ Monit 13:1457–1463
Baldwin AK, Corsi SR, De Cicco LA, Lenaker PL, Lutz MA, Sullivan DJ, Richards KD (2016) Organic contaminants in Great Lakes tributaries: Prevalence and potential aquatic toxicity. Sci Total Environ 554-555:42–52
Bernhard H, Lindell O, Mcclain CJ, Watkins BA, Bruce B, Bachas LG, Wayne S, Claudia T, Suk WA (2012) Nutrition can modulate the toxicity of environmental pollutants: implications in risk assessment and human health. Environ Health Perspect 120:771–774
Eke D, Çelik A, Yilmaz MB, Aras N, Sel SK, Alptekin D (2017) Apoptotic gene expression profiles and DNA damage levels in rat liver treated with perfluorooctane sulfonate and protective role of curcumin. Int J Biol Macromol 104:515–520
Fan XY, Cui BS, Zhao H, Zhang ZM, Zhang HG (2010) Assessment of river water quality in Pearl River Delta using multivariate statistical techniques. Procedia Environ Sci 2:1220–1234
Feher IC, Moldovan Z, Oprean I (2016) Spatial and seasonal variation of organic pollutants in surface water using multivariate statistical techniques. Water Sci Technol 74:1726–1735
Gao HX, Cao YH, Liu YL, Liu N, Guan WJ (2017) Study of the genotoxicity of organic extracts from wastewater-irrigated vegetables using in vitro and in vivo biological tests. Environ Sci Pollut Res Int 24:7051–7059
Guan Y, Wang XD, Wong MH, Sun GP, An TC, Guo J, Zhang GX (2017) Evaluation of genotoxic and mutagenic activity of organic extracts from drinking water sources. PloS ONE 12:e0170454
Guppy R, Ackbarali C, Ibrahim D (2019) Toxicity of crude organic extracts from the zoanthid Palythoa caribaeorum: a biogeography approach. Toxicon 167:117–122
Gupta A, Thakur WAS (2018) Biosafety assessment of municipal wastewater after treatment by Serratia sp. WASTVKR1. Int J Environ Sci Techol 15:2095–2106
Huang GX, Sun JC, Zhang Y, Chen ZY, Liu F (2013) Impact of anthropogenic and natural processes on the evolution of groundwater chemistry in a rapidly urbanized coastal area, South China. Sci Total Environ 463-464:209–221
Jones AN, Bridgeman J (2019) A fluorescence-based assessment of the fate of organic matter in water treated using crude/purified Hibiscus seeds as coagulant in drinking water treatment. Sci Total Environ 646:1–10
Kasuba V, Micek V, Pizent A, Lovakovic BT, Zeljezic D, Milic M, Kopjar N (2019) DNA damage in kidney and parenchymal and non-parenchymal liver cells of adult Wwastar rats after subchronic oral treatment with tembotrione. Environ Sci Pollut Res Int 27:1800–1807
Liu BJ, Peng SH, Liao YY, Long WL (2018) The causes and impacts of water resources crises in the Pearl River Delta. J Clean Prod 177:413–425
Lu WQ, Chen XN, Yue F, Jenter C, Gminski R, Li XY, Xie H, Mersch-Sundermann V (2002) Studies on the in vivo and in vitro mutagenicity and the lipid peroxidation of chlorinated surface (drinking) water in rats and metabolically competent human cells. Mutat Res 513:151–157
Negin S, Gokel MR, Patel MB, Sedinkin SL, Gokel GWJRA (2014) The aqueous medium-dimethylsulfoxide conundrum in biological studies. RSC Adv 5:8088–8093
Renz L, Volz C, Michanowicz D, Ferrar K, Christian C, Lenzner D, El-Hefnawy T (2013) A study of parabens and bisphenol A in surface water and fish brain tissue from the Greater Pittsburgh Area. Ecotoxicology 22:632–641
Rosenmai AK, Lundqvist J, le Godec T, Ohlsson Å, Tröger R, Hellman B, Oskarsson A (2018) In vitro bioanalysis of drinking water from source to tap. Water Res 139:272–280
Sahoo MM, Patra KC, Swain JB, Khatua KK (2016) Evaluation of water quality with application of Bayes’ rule and entropy weight method. Eur J Environ Civ En 21:730–752
Salles FJ, de Toledo MC, César AC, Ferreira GM, Barbério A (2016) Cytotoxic and genotoxic assessment of surface water from São Paulo State, Brazil, during the rainy and dry seasons. Ecotoxicology 25:633–645
Shahzad K, Khan MN, Jabeen F, Kosour N, Ahmad N (2018) Toxicity of zinc oxide nanoparticles (ZnO-NPs) in tilapia (Oreochromis mossambicus): tissue accumulation, oxidative stress. Int J Environ Sci Technol 16:1973–1984
Sun HW, An TC, Li GY, Qiao M, Wei DB (2014) Distribution, possible sources, and health risk assessment of SVOC pollution in small streams in Pearl River Delta, China. Environ Sci Pollut Res Int 21:10083–10095
Taher MA, Rezaeipour E, Afzali D (2004) Anodic stripping voltammetric determination of bismuth after solid-phase extraction using amberlite XAD-2 resin modified with 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol. Talanta 63:797–801
Vazquez Boucard C, Lee-Cruz L, Mercier L, Ramírez Orozco M, Serrano Pinto V, Anguiano G, Cazares L, Díaz D (2017) A study of DNA damage in buccal cells of consumers of well- and/or tap-water using the comet assay: assessment of occupational exposure to genotoxicants. Environ Mol Mutagen 58:619–627
Yang LH, Song XF, Yuan RQ, Ying M, Han DM, Bu HM (2012) A hydrochemical framework and water quality assessment of river water in the upper reaches of the Huai River Basin, China. Environ Earth Sci 67:2141–2153
Zhang YJ, Zhang WJ, Hou J, Wang X, Lu WH, Zheng HY, Xiong W, Liu JL, Yuan J (2019) Seasonal variations of tris (2-chloroethyl) phosphate and cytotoxicity of organic extracts in water samples from Wuhan, China. J Environ Sci 76:299–309
Acknowledgements
This work was supported by Science and Technology Program of Guangzhou (201904010161); Guangzhou Municipal Science and Technology Project (20191A011063); the National Science Foundation of China (NSFC 31500076).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Wu, Z., Wang, D., Wu, J. et al. DNA damage of human derived liver cell line HL-7702 induced by organic extracts from surface water in Pearl River Delta, China. Ecotoxicology 30, 1325–1332 (2021). https://doi.org/10.1007/s10646-020-02293-2
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10646-020-02293-2