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Toxicity and effects of four insecticides on Na+, K+-ATPase of western flower thrips, Frankliniella occidentalis

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Abstract

Western flower thrips (WFT), Frankliniella occidentalis, has become an important pest of vegetables worldwide, due to its economic damage to crop production. In order to control WFT, chemical insecticides are widely used. However, WFT has developed a high resistance against many kinds of insecticides. Na+, K+-ATPase, playing an important role in the ionic transmission across the membrane, is commonly considered to be the target of several xenobiotic compounds. However, whether the Na+, K+-ATPase can be used as one of the target sites for controlling WFT is still unknown. In this study, resistance levels of WFT to four insecticides (chlorpyrifos, beta cypermethrin, abamectin, and thiamethoxam) were measured. It was found that all four insecticides exhibited significant inhibitory effects on WFT, especially on nymphs. The activity of Na+, K+-ATPase was estimated after the treatment of four insecticides. Additionally, mRNA expression levels of three Na+, K+-ATPase α-subunit isoforms (X1, X2 and X3) were detected using RT-qPCR. The transcription profile of three Na+, K+-ATPase α-subunit isoforms were diverse after treatment by these four insecticides, which indicated that these isoforms might play different roles in the tolerance to insecticides. The results suggested that Na+, K+-ATPase can obviously be inhibited by these four classes of insecticide, and may serve as the new target for controlling WFT.

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Acknowledgements

This study was funded by the National Natural Science Foundation of China (31501708), the National Natural Science Foundation of China (31501707), the National Key R&D Program of China (2017YFD0200400), the Industrial Development Fund Support Project of Dapeng District (KY20180117), and the Basic Research on Science and Technology Project of Shenzhen (JCYJ20160530191934833).

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Author notes

  1. These authors contributed equally: Tianbo Ding, Sifang Wang

Authors and Affiliations

  1. Key Lab of Integrated Crop Pest Management of Shandong, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, PR China

    Tianbo Ding, Sifang Wang, Changyou Li, Fanghao Wan & Bin Zhang

  2. China-Australia Joint Institute of Agricultural and Environmental Health, Qingdao Agricultural University, Qingdao, 266109, PR China

    Tianbo Ding, Sifang Wang, Changyou Li, Fanghao Wan & Bin Zhang

  3. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China

    Yulin Gao

  4. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, PR China

    Fanghao Wan

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  1. Tianbo Ding
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  2. Sifang Wang
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  3. Yulin Gao
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Ding, T., Wang, S., Gao, Y. et al. Toxicity and effects of four insecticides on Na+, K+-ATPase of western flower thrips, Frankliniella occidentalis. Ecotoxicology 29, 58–64 (2020). https://doi.org/10.1007/s10646-019-02139-6

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