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Esterase-mediated spinosad resistance in house flies Musca domestica (Diptera: Muscidae)

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Abstract

Although esterase-mediated spinosad resistance has been proposed for several insects, the associated molecular mechanism remains poorly understood. In this study, we investigated the mechanism of esterase-based spinosad resistance in house flies using a susceptible strain (SSS) and a spinosad-resistant, near-isogenic line (N-SRS). Combined with the synergistic effect of DEF on spinosad in the N-SRS strain, decreased ali-esterase activity in the spinosad-resistant strain has implicated the involvement of mutant esterase in spinosad resistance in house flies. Examination of the carboxylesterase gene MdαE7 in the two strains revealed that four non-synonymous mutations (Trp251-Leu, Asp273-Glu, Ala365-Val, and Ile396-Val) may be associated with spinosad resistance in house flies. Single nucleotide polymorphism analysis further indicated a strong relationship between these four mutations and spinosad resistance. Moreover, quantitative real-time PCR revealed a female-linked MdαE7 expression pattern in the N-SRS strain, which may contribute to sex-differential spinosad resistance in house flies.

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Acknowledgements

We thank the National Natural Science Foundation of China and the National Key Research and Development Program of China for the financial support of this study.

Funding

This study was funded by the National Natural Science Foundation of China (No. 31672045) and the National Key Research and Development Program of China (2018YFD0200408).

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Authors and Affiliations

  1. State Key Lab Breeding Base for Zhejiang Sustainable Plant Pest Control, MOA Key Lab for Pesticide Residue Detection, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Yi Zhang

  2. Department of Entomology, China Agricultural University, Beijing, 100193, China

    Yi Zhang, Zhuo Ma, Chunmei You, Xiwu Gao & Xueyan Shi

  3. Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs, Beijing, 100125, China

    Mingcheng Guo

  4. Dongcheng Center for Diseases Prevention and Control, Beijing, 100009, China

    Zhuo Ma

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  1. Yi Zhang
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  2. Mingcheng Guo
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  3. Zhuo Ma
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  4. Chunmei You
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  5. Xiwu Gao
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Correspondence to Xiwu Gao or Xueyan Shi.

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Zhang, Y., Guo, M., Ma, Z. et al. Esterase-mediated spinosad resistance in house flies Musca domestica (Diptera: Muscidae). Ecotoxicology 29, 35–44 (2020). https://doi.org/10.1007/s10646-019-02125-y

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